Searching for genes controlling fatness traits in pigs - a review

• Autorzy: Switonski M , Chmurzynska A. , Mackowski M.
• Języki publikacji: EN

Abstrakty

EN

Fat content of carcass is an important multigenic trait in pig breeding. There are reports indicating several chromosomes, e.g. 1, 2, 4, 5, 6, 7, 12, 13, 14 and 18 which possibly harbour QTLs for fatness traits. Among QTL candidate genes there are leptin (LEP) and its receptor (Lepr), both playing essential role in food intake and energy balance. Moreover, expression level and polymorphism of the adipocyte specific transcription factors, such as CREB (cAMP response element binding protein) or C/EBP (CCAAT/enhancer-binding protein) may also cause phenotypic variation of the fatness traits. Some of the candidate QTLs, as Lep, Lepr, C/EBP and additionally H-FABP (fatty acid binding protein gene) and RYR1, are localized on chromosome 6. It is foreseen that searching for polymorphisms of the chosen genes may reveal association between a genotype and phenotypic variation of selected fatness traits. However, the studies are complex and require analysis of numerous genes.Cited are 71 references.

PL

Odkładanie tkanki tłuszczowej jest ważną cechą produkcyjną świń, warunkowaną przez wiele genów. Istnieją doniesienia wskazujące, że w różnych chromosomach (np. 1, 2, 4, 5, 6, 7, 12, 13, 14 i 18) mogą występować geny o dużym efekcie działania (QTL) odpowiadające za zmienność fenotypową tych cech. Genami kandydującymi do roli QTL są m. in. gen leptyny (Lep) i receptora leptyny (Lepr). Ich białkowe produkty odgrywają istotną rolę w kontroli pobierania pokarmu i utrzymywania równowagi energetycznej.Zmienność cech otłuszczenia może być m.in. wywołana zróżnicowanym poziomem ekspresji i polimorfizmu specyficznych dla adipocytów czynników transkrypcyjnych, takich jak CREB (cAMP response elementbinding protein) czy C/EBP (CCAAT/enhancer-binding protein). Kilka potencjalnych QTL, takich jak Lep, Lepr, C/EBP, a także H-FABP i RYR1, zlokalizowanych jest w chromosomie 6. Przewiduje się, że poszukiwanie polimorfizmu wybranych genów może wykryć powiązania między genotypem i obserwowaną zmiennością cech otłuszczenia. Podkreślić jednak należy, że badania takie są złożone i wymagają analizy dużej liczby genów. Powołano się na 71 pozycji piśmiennictwa.

Słowa kluczowe

EN

pig; leptin gene; leptin receptor gene; animal genetics; gene; gene analysis; fatness trait; marker genome; genome map; animal breeding; carcass; carcass fatness; phenotypic variability

• Wydawca: -
• Czasopismo: Animal Science Papers and Reports
• Rocznik: 2003
• Tom: 21
• Numer: 2
• Opis fizyczny: p.73-86

Twórcy

autor

Switonski M

• Agricultural University, Wolynska 33, 60-637 Poznan, Poland

autor

Chmurzynska A.

autor

Mackowski M.

Bibliografia

• 1. ANDERSSON L., HALEY C.S., ELLEGREN H., KNOTT S. A., JOHANSSON M., ANDERSSON K., ANDERSSON-ECLUND L., EDFORS-LILIA I., FREDHOLM M., HANSSON I., HAKANSON J., LUNDSTROM K., 1994 – Genetic mapping of quantitative trait loci for growth and fatness in pigs. Science 263, 1771-1774.
• 2. BERKOWITZ L.A., GILMAN M.Z. 1990 – Two distinct forms of active transcription factor CREB (cAMP response element binding protein). Proceedings of the National Academy of Sciences, USA 87, 5258-5262.
• 3. BIDWELL C.A., JI S., FRANK G.R., CORNELIUS S.G., WILLIS G.M., SUPRLOCK M.E.,1997 – Cloning and expression of the porcine obese gene. Animal Biotechnology 8 (2), 191-206.
• 4. CLARKE S.D., THUILLIER P., BAILLIE R.A., SHA X., 1999 – Peroxisome proliferator-activated receptors: a family of lipid-activated transcription factors. American Journal of Clinical Nutrition 70, 566-571.
• 5. CHEN H., CHARLAT O., TARTAGLIA L.A., WOOLF E.A., WENG X., ELLIS S.J., LAKEY N.D., CULPEPPER J., MOORE K.J., BREITBART R.E., DUYK G.M., TEPPER R.I., MORGENSTERN J.P., 1996 – Evidence that the diabetes gene encodes the leptin receptor: identification of a mutation in the leptin receptor gene in db/db mice. Cell 84, 491-495.
• 6. DARLINGTON G.J., ROSS S.E., MACDOUGALD O.A., 1998 – The role of C/EBP genes in adipocyte differentiation. Journal of Biological Chemistry 273, 30057-30060.
• 7. DAVOLI R., FONTANESI L., ZAMBONELLI P., BIGI D., GELLIN J., YERLE M., MILC J.,BRAGLIA S.,CENCI V., CAGNAZZO M., RUSSO V., 2002 – Isolation of porcine expressed sequence tags for the construction of a first genomic transcript map of the skeletal muscle in pig.Animal Genetics 33, 3-18.
• 8. ERICKSON R.L., HEMATI N., ROSS S.E., MACDOUGALD O.A., 2001 – p300 coactivates the adipogenic transcription factor CAAT/enhancer-binding protein α. Journal of Biological Chemistry 276, 16348-16355.
• 9. FAJAS L., AUBOEUF D., RASPE E., SCHOONJAN K., LEFEBVRE A.M., SALADIN R., NAJIB J., LAVILLE M., FRUCHART J.C., DEEB S., VIDAL-PUIG A., FLEIR J., BRIGGS M.R.,STAELS B., VIDAL H., AUWERX J., 1997 – The organization, promoter analysis, and expression of the human PPARγ gene. Journal of Biological Chemistry 272, 18779-18789.
• 10. FAJAS L., FRUCHART J.C., AUWERX J., 1998 – PPARγ3 mRNA: a distinct PPARγ mRNA subtype transcribed from an independent promoter. FEBS Letters 438, 55-60.
• 11. FARBER C.R., MEDRANO J.F., 2003 – Putative in silico mapping of DNA sequences to livestock genome maps using SSLP flanking sequences. Animal Genetics 34, 11-18.
• 12. FRONICKE L., CHOWDHARY B.P., SCHERTHAN H., GUSTAVSSON I., 1996 – A comparative map of the porcine and human genomes demonstrates ZOO-FISH and gene mapping-based chromosomal homologies. Mammalian Genome 7, 285-290.
• 13. FRONICKE L., WIENBERG J., 2001 – Comparative chromosome painting defines the high rate of karyotype changes between pigs and bovids. Mammalian Genome 12, 442-449.
• 14. GERBENS F., DE KOENING D.J., HARDERS F., MEUWISSEN T.H.E., JANSS L.L.G.,GROENEN M.A.M., VEERKAMP J.H., TEPAS M.F.W., 2000 – The effect of adipocyte and heart fatty acid-binding protein genes on intramuscular fat and backfat content in Meishan crossbred pigs.Journal of Animal Science 78, 552-559.
• 15. GONG D-W., BI S., PRATLEY R.E., WEINTRAUB B.D., 1996 – Genomic structure and promoter analysis of the human obese gene. Journal of Biological Chemistry 271, 3971-3974.
• 16. GONZALEZ G.A., YAMAMOTO K.K., FISCHER W.H., KARR D., MENZEL P., BIGGS W.,VALE W.W., MONTMINY M.R., 1989 – A cluster of phosphorylation sites on the cyclic AMP-regulated nuclear factor CREB predicted by its sequence. Nature 337, 749-752.
• 17. GOUREAU A., GARRIGUES A., TOSSER-KLOPP G., LAHBIB-MANSAIS Y., CHARDON P.,YERLE M., 2001 – Conserved synteny and gene order difference between human chromosome 12 and pig chromosome 5. Cytogenetics and Cell Genetics 94, 49-54.
• 18. GOUREAU A., YERLE M., SCHMITZ A., RIQUET J., MILAN D., PINTON P., FRELAT G.,GELLIN J., 1996 – Human and porcine correspondence of chromosome segments using bidirectional chromosome painting. Genomics 36, 252-62.
• 19. GREGOIRE F.M., SMAS C.M., SUL H.S., 1998 – Understanding adipocyte differentiation.Physiological Reviews 78, 783-809.
• 20. GRINDFLEK E., SZYDA J., LIU Z., LIEN S., 2001 – Detection of quantitative trait loci for meat quality in a commercial slaughter pig cross. Mammalian Genome 12, 299-304.
• 21. HARLIZIUS B., RATTINK A.P., DE KONING D.J., FAIVRE M., JOOSTEN R.G., VAN ARENDONK J.A.M., GROENEN M.A.M., 2000 – The X chromosome harbors quantitative trait loci for backfat thickness and intramuscular content in pigs. Mammalian Genome 11, 800-802.
• 22. HOEFFLER J.P., MEYER T.E., WAEBER G., HABENER J.H., 1990 – Multiple adenosine 3’,5’-monophosphate response element DNA-binding proteins generated by gene diversification and alternative exon splicing. Molecular Endocrinology 4, 920-930.
• 23. HOLLENBERG A.N., SUSULIC V.S., MADURA J.P., ZHANG B., MOLLER D.E., TONTONOZ P., SARRAF P., SPIEGELMAN B.M., LOWELL B.B., 1997 – Functional antagonism between CCAAT/enhancer binding protein α and peroxisome proliferator-activated receptor ă on the leptin promoter. Journal of Biological Chemistry 272, 5283-5290.
• 24. HWANG CH.-S., MANDRUP S., MACDOUGALD O.A., GEIMAN D.E., 1996 – Transcriptional activation of the mouse obese (ob) gene by CCAAT/enhancer binding protein α. Proceedings of the National Academy of Sciences, USA 93, 873-877.
• 25. ISSE N., OGAWA Y., TAMURA N., MASUZAKI H., MORI K., OKAZAKI T., SATOH N.,SHIGEMOTO M., YOSHIMASA Y., NISHI S., HOSODA K., INAZAWA J., NAKAO K., 1995 – Structural organization and chromosomal assignment of the human obese gene. Journal of Biological Chemistry 270 (46), 27728-27733.
• 26. JIANG Z-H., GIBSON J.P., 1999 – Genetic polymorphisms in the leptin gene and their association with fatness in four pig breeds. Mammalian Genome 10, 191-193.
• 27. JUGE-AUBRY C.E., GORLA-BAJSZCZAK A., PERNIN A., LEMBERGER T., WAHLI W.,BURGER A.G., MEIER C.A., 1995 – Peroxisome proliferator-activated receptor mediates cross-talk with thyroid hormone receptor by competition for retinoid X receptor. Journal of Biological Chemistry 270, 18117-18122.
• 28. KARSENTY E., BARILLOT E., TOSSER-KLOPP G., LAHBIB-MANSAIS Y., MILAN D.,HATEY F., CIRERA S., SAWERA M., JORGENSEN C.B., CHOWDHARY B., FREDHOLM M.,WIMMERS K., PONSUKSILI S., DAVOLI R., FONTANESI L., BRAGLIA S., ZAMBONELLI P., BIGI D., NEUENSCHWANDER S., GELLIN J., 2003 – The GENETPIG database: a tool for comparative mapping in pig (Sus scrofa). Nucleic Acid Research 31, 138-141.
• 29. KENNES Y.M., MURPHY B.D., POTHIER F., PALIN M.-F., 2001 – Characterization of swine leptin (LEP) polymorphisms and their association with production traits. Animal Genetics 32, 215-218.
• 30. KIM J., LU J., QUINN P.G., 2000 – Distinct cAMP response element-binding protein (CREB) domains stimulate different steps in a concerted mechanism of transcription activation. Proceedings of the National Academy of Sciences USA 97, 11292-11296.
• 31. KOPEČNY M., STRATIL A., ČEPICA S., 1997 – Polymorphism at the porcine LEPR gene detected by PCR-DGGE. Animal Genetics 28, 461.
• 32. KŘENKOWA L., KUCIEL J., URBAN T., 1999 – Association of the RYR1, GH, LEP and TF genes with carcass and meat quality traits in pigs. Czech Journal of Animal Science 44, 481-486.
• 33. KULIG H., GRZESIAK W., SZATKOWSKA I., 2001 – Effect of leptin gene polymorphism on growth and carcass traits in pig. Archiv Tierzucht Dummerstorf 44 (3), 291-296.
• 34. KURYŁ J., 2000 – Geny cech ilościowych zwierząt gospodarskich – aktualny stan badań (The current state of research on the quantitative traits loci in farm animals). In Polish with English summary.Prace i Materiały Zootechniczne 56, 7-50.
• 35. KURYŁ J., KAPELAŃSKI W., PIERZCHAŁA M., BOCIAN M., GRAJEWSKA S., 2003 – A relationship between genotypes at the GH and LEP loci and carcass meat and fat deposition in pigs.Animal Science Papers and Reports 21, 15-26.
• 36. KURYŁ J., ŻURKOWSKI M., RÓŻYCKI M., DUNIEC M., PIERZCHAŁA M., KOSSAKOWSKA A., JANIK A., KAMYCZEK M., SZYDŁOWSKI M., CYMEROWSKA-PROKOPCZYK I.,CZERWIŃSKI S., ŚWITOŃSKI M., 1998 – Genes affecting meat and fat content in carcass of pig – recapitulation of Polish project ”Pig Genome Mapping”. Polish Journal of Food and Nutrition Sciences 7/48, 70-76.
• 37. LEKSTROM-HIMES J., XANTHOPOULOS K.G., 1998 – Biological role of the CCAAT/enhancerbinding protein family of transcription factors. Journal of Biological Chemistry 273, 28545-28548.
• 38. LIN F.T., MACDOUGALD O.A., DIEHL A.M., LANE M.D., 1993 – A 30-kDa alternative translation product of the CCAAT/enhancer binding protein α message: Transcriptional activator lacking antimitotic activity. Proceedings of the National Academy of Sciences, USA 90, 9606-9610.
• 39. MADEJA Z., PIASECKA A., LECHNIAK D., ŚWITOŃSKI M., 2002 – Leptyna – polimorfizm genetyczny i rola w rozrodzie (Leptin and its role in reproduction and gene polymorphism). In Polish with English summary. Medycyna Weterynaryjna 58, 572-576.
• 40. MALEK M., DEKKERS J.C.M., LEE H.K., BAAS T.J., ROTHSCHILD M.F., 2001a – A molecular genome scan analysis to identify chromosomal regions influencing economic traits in the pig. I.Growth and body composition. Mammalian Genome 12, 630-636.
• 41. MALEK M., DEKKERS J.C.M., LEE H.K., BAAS T.J., PRUSA K., HUFF-LONERGAN E., ROTHSCHILD M.F., 2001b – A molecular genome scan analysis to identify chromosomal regions influencing economic traits in the pig. II. Meat and muscle composition. Mammalian Genome 12,637-645.
• 42. MAMMES O., BETOULLE D., AUBERT R., HERBETH B., SIEST G., FUMERON F., 2000 – Association of the G-2548A polymorphism in the 5’ region of the LEP gene with overweight. Annals of Human Genetics 64, 391-394.
• 43. MASON M.M., HE Y., CHEN H., QUON J., REITMAN M., 1998 – Regulation of leptin promoter function by Sp1, C/EBP, and a novel factor. Endocrinology 139 (3), 1013-1022.
• 44. MILAN D., BIDANEL J.-P., IANNUCCELLI N., RIQUET J., AMIGUES Y., GRUAND J., LE ROY P., RENARD C., CHEVALET C., 2002 – Detection of quantitative trait loci for carcass composition traits in pigs. Genetics, Selection, Evolution 34, 705-728.
• 45. MILLER S.G., DEVOS P., GUERRE-MILLO M., WONG K., HERMANN T., STAELS B., BRIGGS M.R., AUWERX J., 1996 – The adipocyte specific transcription factor C/EBP α modulates human ob gene expression. Proceedings of the National Academy of Sciences, USA 93, 5507-5511.
• 46. MUKHERJEE R., JOW L., CROSTON G.E., PATERNITI J.R., 1997 – Identification, characterization,and tissue distribution of human peroxisome proliferator-activated receptor (PPAR) isoforms PPARγ2 versus PPARγ1 and activation with retinoid X receptor agonists and antagonists. Journal of Biological Chemistry 272, 8071-8076.
• 47. NEUENSCHWANDER S., RETTENBERGER G., MEIJERINK E., JÖRG H., STRANZINGER G., 1996 – Partial characterization of obesity gene (OBS) and its localization to chromosome 18 by somatic cell hybrids. Animal Genetics 27, 275-278.
• 48. OSSIPOW V., DESCOMBES P., SCHIBLER U., 1993 – CCAAT/enhancer-binding protein mRNA is translated into multiple proteins with different transcription activation potentials. Proceedings of the National Academy of Sciences, USA 90, 8219-8223.
• 49. OVILO C., OLIVER A., NOGUERA J.L., CLOP A., BARRAGAN C., VARONA L., RODRIGUEZ C., TORO M., SANCHEZ A., PEREZ-ENCISCO M., SILIO L., 2002 – Test for positional candidate genes for body composition on pig chromosome 6. Genetics, Selection, Evolution 34, 465-479.
• 50. OVILO C., PEREZ-ENCISO M., BARRAGAN C., CLOP A., ROGRIGUEZ C., OLIVER M.A.,TORO M.A., NOGUERA J.L., 2000 – A QTL for intramuscular fat and backfat thickness is located on porcine chromosome 6. Mammalian Genome 11, 344-346.
• 51. QUINN P., 2002 – Mechanisms of basal and kinase-inducible transcription activation by CREB.Progress in Nucleic Acids and Molecular Biology 72, 269-306.
• 52. RATTINK A.P., DE KONING D.J., FAIVRE M., HARLIZIUS B., VAN ARENDONK J.A.,GROENEN M.A., 2000 – Fine mapping and imprinting analysis for fatness trait QTLs in pigs. Mammalian Genome 11, 656-661.
• 53. REUSCH J.E., COLTON L.A., KLEMM D.J., 2000 – CREB activation induces adipogenesis in 3T3-L1 cells. Molecular and Cellular Biology 20, 1008-1020.
• 54. ROSEN E.D., HSU C.H., WANG X., SAKAI S., FREEMAN M.W., GONZALEZ F.J., SPIEGELMAN B.M., 2002 – C/EBPα induces adipogenesis through PPAR γ: a unified pathway. Genes & Development 16, 22-26.
• 55. ROSEN E.D., SPIEGELMAN B.M., 2001 – PPARγ: a nuclear regulator of metabolism, differentiation,and cell growth. Journal of Biological Chemistry 276, 37731-37734.
• 56. ROSEN E.D., WALKEY C.J., PUIGSERVER P., SPIEGELMAN B.M., 2000 – Transcriptional regulation of adipogenesis. Genes & Development 14, 1293-1307.
• 57. RUIZ-CORTEZ Z.T., MEN T., PALIN M.F., DOWNEY B.R., LACROIX D.A., MURPHY B.D.,– 2000 Porcine leptin receptor: molecular structure and expression in the ovary. Molecular Reproduction and Development 56:465-74.
• 58. RUPPERT S., COLE T.J., BOSHART M., SCHMIDT E., SCHUTZ G., 1992 – Multiple mRNA isoforms of the transcription activator protein CREB: generation by alternative splicing and specific expression in primary spermatocytes. EMBO Journal 11, 1503-1512.
• 59. SALADIN R., FAJAS L., DANA S., HALVORSEN Y.D., AUWERX J., BRIGGS M., 1999 – Differential regulation of peroxisome proliferator activated receptor γ1 (PPARγ1) and PPARγ2 messenger RNA expression in the early stages of adipogenesis Cell Growth & Differentiation 10, 43-48.
• 60. SCHMITZ A., OUSTRY A., VAIMAN D., CHAPUT B., FRELAT G., CRIBIU E.P., 1998 – Comparative karyotype of pig and cattle using whole chromosome painting probes. Hereditas 128, 257-263.
• 61. SCHWERIN M., 2001 – Structural and functional genomics in domestic animals: the way to understand the phenotype. Journal of Applied Genetics 42, 293-308.
• 62. SOUKAS A., SOCCI N.D., SAATKAMP B.D., NOVELLI S., FRIEDMAN J.M., 2001 – Distinct transcriptional profiles of adipogenesis in vivo and in vitro. Journal of Biological Chemistry 276,34167-34174.
• 63. STRATIL A., KOPEČNY M., MOSER G., SCHRÖFFEL J., ČEPICA S., 1998 – HpaII and RsaI PCR-RFLPs within an intron in the porcine leptin receptor gene (LEPR) and its linkage mapping.Animal Genetics 29, 405-406.
• 64. STRATIL A., PEELMAN L., VAN POUCKE M., ČEPICA S., 1997 – A HinfI PCR-RFLP at the porcine leptin (LEP) gene. Animal Genetics 28, 371-372.
• 65. SUN H.F., ERNST C.W., YERLE M., PINTON P., ROTHSCHILD M.F., CHARDON P., ROGELGAILLARD C., TUGGLE C.K., 1999 – Human chromosome 3 and pig chromosome 13 show complete synteny conservation but extensive gene-order differences. Cytogenetics and Cell Genetics 85, 273-278.
• 66. TARTAGLIA L.A., DEMBSKI M., WENG X., DENG N., CULPEPPER J., DEVOS R., RICHARDS G.J., CAMPFIELD L.A., CLARK F.T., DEEDS J., MUIR C., SANKER S., MORIARTY A., MOORE K.J., SMUTKO J.S., MAYS E.A., WOOLF E.A., MONROE C.A., TEPPER R.I.,1995 – Identification and expression cloning of a leptin receptor, OB-R. Cell 83, 1263-1271.
• 67. TONTONOZ P., HU E., GRAVES R.A., BUDAVARI A.I., SPIEGELMAN B.M., 1994 – mPPAR gamma 2: tissue-specific regulator of an adipocyte enhancer. Genes & Development 8, 1224-1234.
• 68. TUGGLE C.K, GENET C., SHI X.W., CHARDON P., SANCHEZ-SERRANO I., CRAVENS G.,MILAN D., YERLE M., 2001 – Cytogenetic and radiation hybrid mapping reveals conserved synteny and gene order between human chromosome 21 and pig chromosome 13. Mammalian Genome 12,397-399.
• 69. VALVE R., SIVENIUS K., MIETTINEN R., PIHLAJAMAKI J., RISSANEN A., DEEB S.S., AUWERX J., UUSITUPA M., LAAKSO M., 1999 – Two polymorphisms in the peroxisome proliferator-activated receptor γ gene are associated with severe overweight among obese women. Journal of Biological Chemistry 84, 3708-3712.
• 70. VINCENT A.L., WANG L., ROTHSCHILD M.F., 1997 – A restriction fragment length polymorphism in the porcine leptin receptor (LEPR) gene. Journal of Animal Science 75, 2287.
• 71. ZHANG Y., PROENCA R., MAFFEI M., BARONE M., LEOPOLD L., FRIEDMAN J.M., 1994 – Positional cloning of the mouse obese gene and its human homologue. Nature 372, 425-431.