Insertion of GPI-anchored alkaline phosphatase into supported membranes: a combined AFM and fluorescence microscopy study

• Autorzy: Rieu J. P. , Ronzon F. , Place C. , Dekkiche F. , Cross B. , Roux B.
• Języki publikacji: EN

Abstrakty

EN

A new method based on combined atomic force microscopy (AFM) and fluorescence microscopy observations, is proposed to visualize the insertion of glycosylphos- phatidyl inositol (GPI) anchored alkaline phosphatase from buffer solutions into sup­ported phospholipid bilayers. The technique involves the use of 27 nm diameter fluo­rescent latex beads covalently coupled to the amine groups of proteins. Fluorescence microscopy allows the estimation of the relative protein coverage into the membrane and also introduces a height amplification for the detection of protein/bead com­plexes with the AFM. The coupling of the beads with the amine groups is not specific; this new and simple approach opens up new ways to investigate proteins into sup­ported membrane systems.

Słowa kluczowe

EN

phospholipid bilayer; glycosylphosphatidyl inositol; biological activity; biomembrane; supported lipid membrane; atomic force microscopy; alkaline phosphatase; fluorescent bead; dipalmitoylphosphatidylcholine; new method; fluorescence microscopy observation; membrane protein

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2004
• Tom: 51
• Numer: 1
• Opis fizyczny: p.189-197,fig.,ref.

Twórcy

autor

Rieu J. P.

• Universite Claude Bernard Lyon-1, 69622 Villeurbanne, France

autor

Ronzon F.

autor

Place C.

autor

Dekkiche F.

autor

Cross B.

autor

Roux B.

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