PL
W pracy określono wpływ obecności i stężenia jonów Pb (II) i Cd (II) oraz stężenia substratu na aktywność pepsyny i trypsyny w warunkach in vitro. Stwierdzono znaczący wzrost aktywności pepsyny ze wzrostem stężenia jonów tych metali w układzie reakcyjnym, natomiast duży nadmiar substratu w stosunku do enzymu obniżał aktywność obu enzymów.
EN
The objective of this study was to investigate the effect of the presence and concentration of Pb(II) and Cd(II) ions, as well as the substrate/enzyme ratio on pepsin and trypsin activities in vitro. The experimental design was a combination of three concentrations (1, 10 and 100 µg/4 cm3) of the metal ions and two substrate/enzyme rations (S/E = 10 and 100 for pepsin and 100 and 1000 for trypsin). The enzymatic activities were determined by the Folin method that is based on the colorimetric reaction of tyrosine with the Folin reagent. It was found that Pb2+ and Cd2+ ions significantly activated pepsin. The degree of this activation increased with increasing metal ion concentration in the experimental milieu. The presence and concentration of the analysed cations did not affect trypsin activity. The substrate/enzyme ratio influenced the activity of both proteases. Higher protein vs. enzyme excess reduced pepsin and trypsin activities in vitro.