Evaluation of the immunogenicity of the vaccine against Pasteurella multocida infections in calves

• Autorzy: Borkowska-Opacka B , Kedrak A. , Truszczynski M.
• Języki publikacji: EN

Abstrakty

EN

Evaluation of the immunogenicity of the vaccine against respiratory tract infections in calves caused by Pasteurella multocida, and prepared from antigen of a local strain of serotype 3 with oil adjuvant addition, was the object of the prescnt studies. Two experiments were carried out using calves. Vaccine was given them at a dose of 2 ml i.m., twice every 14 days. In experiment 1, sera were obtained before vaccination, 2 and 5 weeks after the first vaccination, in experiment 2 - before vaccination, 2 weeks, 1 and 2 months after the first vaccination. lmmunogenicity of the vaccine was evaluated by means of an agar gel immunodiffusion (AGID) test and an ELISA test. The carrier-state of Pasteurella multocida on mucous membrane of nasal cavity was examined. lt was found that immunisation stimulated the production of the specific precipitins for serotype 3 and it caused the increase of IgG level found using ELISA test with microplate coated with whole-bacterium antigen or LPS of that serotype. The investigation confirms the good immunogenicity of the vaccine.

Słowa kluczowe

EN

calf; respiratory tract; infection; vaccine; ELISA test; immunogenicity; Pasteurella multocida

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 1999
• Tom: 43
• Numer: 2
• Opis fizyczny: p.155-166,fig.

Twórcy

autor

Borkowska-Opacka B

• National Veterinary Research Institute, 24-100 Pulawy, Poland

autor

Kedrak A.

autor

Truszczynski M.

Bibliografia

• 1. Atsumi F., Toshiyuki O., Zhao H.K., Hiramune T., Kikuchi N., Kiowa M., Takahashi H.: J. Coll. Dairying 11, 349, 1986.
• 2. Baljer G., Klein G., Waldner D., Kunz W., Büttner M., Mayr A.: Tierärztl. Umsch. 43, 474, 1988.
• 3. Bechtol D.T., Jones G.F.: Vet. Med. 91, 1042, 1996.
• 4. Bennett B.W.: Bovine Prac. 3, 26, 1982.
• 5. Blackburn B.O., Heddleston K.L., Pfow C.J.: Avian Dis. 19, 353, 1975.
• 6. Borkowska-Opacka B., Kędrak A., Truszczyński M., Rutkowska-Jurga I., Czerski H.: Serotypes of Pasteurclla multocida strains isolated from cattle. Materiały X Kongresu PTNW, 19-21.09.1996, p.259.
• 7. Borkowska-Opacka B., Kędrak A., Truszczyński M.: Bull. Vet. Inst. Pulawy 41, 95, 1997.
• 8. Cardella M.A., Adviento M.A., Nervig R.M.: Can. J. Vet. Res. 51, 204, 1987.
• 9. Catt D.M., Chengappa M.M., Kadel W.L., Herren C.E.: Can. J. comp. Med. 49, 366, 1985.
• 10. Chengappa M.M., McLaughlin B.G., Kadet W.L., Maddux R.L., Greer S.C.: Vet. Microbiol. 21, 147, 1989.
• 11. De Alwis M.C.L.: Haemorrhagic septicaemia. In: Manual of standards for diagnostic tests and vaccines, OIE, 1992, p.313.
• 12. Donachie W.: Br. vet. J. 148, 93, 1992.
• 13. Erler W., Schönherr W., Heilmann P., Kielstein P., Müller G., Feist H., Flossmann K.D.: Mh. Vet.-Med. 38, 87, 1983.
• 14. Erler W., Diller R., Steinbach G.: Mh. Vet.-Med. 46, 498, 1991.
• 15. Erler W., Schönherr W.: Mh. Vet.-Med. 48, 119, 1993.
• 16. Frank G.H.: Pasteurellosis of Cattle. In: Pasteurella and Pasteurellosis, Ed. Adlam C., Rutter J.M., Acad. Press, 1989, p. 197.
• 17. Grossmann E., Erler W., Schimmel D.: Berl. Münch. tierärztl. Wschr. 11, 90, 1998.
• 18. Heddleston K.L., Gallagher J.E., Rebers P.A.: Avian Dis. 16, 925, 1972.
• 19. Khalil A.A., Kita J.: Annals Warsaw Agricultural University SGGW AR 11, 77, 1983.
• 20. Kielstein P., Schimmel D.: Mh. Vet.-Med. 38, 83, 1983.
• 21. Klaassen J.M., Bernard B.L., DiGiacomo R.F.: J. Clin. Microbiol. 21, 617, 1985.
• 22. Martin S.W.: Can. Vet. J. 24, 10, 1983.
• 23. Martin S.W.: Can. Vet. J. 25, 44, 1984.
• 24. Martin W., Acres S., Janzen E., Willson P., Allen B.: Can. J. 25, 145, 1984.
• 25. Mosier D.A., Confer A.W., Panciera R.J.: Res. vet. Sci. 47, 1, 1989.
• 26. Namioka S., Murata M.: Cornell Vet. 51, 498, 1961.
• 27. Namioka S., Murata M.: Cornell Vet. 51, 507, 1961.
• 28. Ose E.E., Muenster B.S.: Am. J. vet. Res. 29, 1863, 1968.
• 29. Purdy C.W., Livingston C.W., Frank G.H., Cummins J.M., Cole N.A., Loan R.W.: J. Am. vet. med. Ass. 6, 589, 1986.
• 30. Schimmel D., Kielstein P., Bruer W., Putsche R., Slucka R.: Mh. Vet.-Med. 38, 81, 1983.
• 31. Schimmel D., Erler W., Feist H.: Dt. Tierärztl. Wschr. 99, 165, 1992.
• 32. Westphal O., Jann K.: Bacterial lipopolysaccharides. Extraction with phenol-water and further applications of the procedure. In: Methods in carbohydrate chemistry, Eds. Whistler R.L., Wolform M.L., Acad. Press, London, 5, 1965, p.83