Development of embryo-like structures in the suspension cultures of flax coincides with secretion of chitinase-like proteins

• Autorzy: Petrovska B. , Salaj T. , Moravcikova J. , Libantova J. , Salaj J.
• Języki publikacji: EN

Abstrakty

EN

Flax suspension cultures have been established from the callus induced from the cotyledons, hypocotyls, and immature zygotic embryos (iZE). The growth of flax suspension culture (expressed as a sedimented cell volume) was compared in both conditioned (by liquid from embryogenic Pinus nigra suspension culture) and nonconditioned media. Conditioning of media significantly increased the growth of the cell lines of hypocotyl and iZE origin; however, it had no promotive effect on embryogenic response of these flax liquid cultures. Formation of embryo-like structures (ELS), confirmed also histologically, has only been found in the cell line derived from iZE and cultivated in non-conditioned MS medium supplemented with 1 mg l⁻¹ 2,4-D. The process of ELS formation in this cell line was accompanied by the expression of the protein(s) with chitinolytic activity and molecular weight approximately of 25 kDa. The relationship between the formation of ELS and secretion of chitinase(s) is discussed.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2010
• Tom: 32
• Numer: 4
• Opis fizyczny: p.651-656,fig.,ref.

Twórcy

autor

Petrovska B.

• Institute of Plant Genetics and Biotechnology, Slovak Academy of Sciences, Akademicka´ 2, P. O. Box 39 A, 950 07 Nitra 1, Slovak Republic

autor

Salaj T.

• Institute of Plant Genetics and Biotechnology, Slovak Academy of Sciences, Akademicka´ 2, P. O. Box 39 A, 950 07 Nitra 1, Slovak Republic

autor

Moravcikova J.

• Institute of Plant Genetics and Biotechnology, Slovak Academy of Sciences, Akademicka´ 2, P. O. Box 39 A, 950 07 Nitra 1, Slovak Republic

autor

Libantova J.

• Institute of Plant Genetics and Biotechnology, Slovak Academy of Sciences, Akademicka´ 2, P. O. Box 39 A, 950 07 Nitra 1, Slovak Republic

autor

Salaj J.

• Institute of Plant Genetics and Biotechnology, Slovak Academy of Sciences, Akademicka´ 2, P. O. Box 39 A, 950 07 Nitra 1, Slovak Republic

Bibliografia

• Arie M, Hikichi K, Takahashi K, Esaka M (2000) Characterisation of a basic chitinase which is secreted by cultured pumpkin cells. Physiol Plant 110:232–239
• Ben Amar A, Cobanov P, Boonrod K, Krczal G, Bouzid S, Ghorbel A, Reuste GM (2007) Efficient procedure for grapevine embryogenic suspension establishment and plant regeneration: role of conditioned medium for cell proliferation. Plant Cell Rep 26:1439–1447
• Bishop-Hurley SL, Gardner RC, Walter C (2003) Isolation and molecular characterization of genes expressed during somatic embryo development in Pinus radiata. Plant Cell Tissue Organ Cult 74:267–281
• Chugh A, Khurana P (2002) Gene expression during somatic embryogenesis—recent advances. Curr Sci 83:715–730
• Cunha ACG, Ferreira MF (1996) Somatic embryogenesis, organogenesis and callus growth kinetics of flax. Plant Cell Tissue Organ Cult 47:1–8
• de Vries SC, Booij H, Janssens R, Ronald V, Saris L, Lo Schiavo F, Terzi M, van Kammen A (1988) Carrot somatic embryogenesis depends on the phytohormone-controlled presence of correctly glycosilated extracellular proteins. Genes Dev 2:462–476
• Domon J-M, Neutelings G, Roger D, David A, David H (2000) A basic chitinase-like protein secreted by embryogenic tissues of Pinus caribea acts on arabinogalactan proteins extracted from the same cell lines. J Plant Physiol 156:33–39
• Dyachok JV, Wiveger M, Kenne L, von Arnold S (2002) Endogenous nod-factor-like signal molecules promote early somatic embryo development in Norway spruce. Plant Physiol 128:523–533
• Gupta PK, Durzan DJ (1985) Shoot multiplication from mature trees of Douglas fir (Pseudotsuga menziesii) and sugar pine (Pinus lambertiana). Plant Cell Rep 4:177–179
• Helleboid S, Hendriks T, Bauw G, Inze D, Vasseur J, Hilbert J-L (2000) Three major somatic embryogenesis related proteins in Cichorium identified as PR proteins. J Exp Bot 51:1189–1200
• Kasprzewska A (2003) Plant chitinases—regulation and function. Cell Mol Biol Lett 8:809–824
• Kragh KM, Hendriks T, de Jong AJ, Lo Schiavo F, Bucherna N, Hojrup P, Mikkelsen JD, de Vries S (1996) Characterisation of chitinases able to rescue somatic embryos of the temperature-sensitive carrot variant ts11. Plant Mol Biol 31:631–645
• Ma Y, Sawhney VK, Steeves TA (1992) Staining of paraffin-embedded plant material in safranin and fast green without prior removal of the paraffin. Can J Bot 71:996–999
• Maës O, Coutos-Thevenot P, Jouenne T, Boulay M, Guen J (1997) Influence of extracellular proteins, proteases and protease inhibitors on grapevine somatic embryogenesis. Plant Cell Tissue Organ Cult 50:97–105
• Matthys-Rochon E (2005) Secreted molecules and their role in embryo formation in plants: a mini review. Acta Biol Crac Ser Bot 47:23–29
• Mo LH, Egersdotter U, von Arnold S (1996) Secretion of specific extracellular proteins by somatic embryos of Picea abies is dependent on embryo morphology. Ann Bot 77:143–152
• Pan QS, Ye SX, Kuć J (1991) A technique for detection of chitinase, β-1,3-glucanase, and protein patterns after a single separation using polyacrylamide gel electrophoresis or isoelectrofocusing. Phytopathology 81:970–974
• Petrovská B (2006) Molecular-biological aspects of flax embryogenesis. PhD thesis, Nitra, 156 p
• Petrovská B, Preťová A, Salaj J (2001) Embryo-like structures induced from cultured flax (Linum usitatissimum L.) hypocotyl segments. In: Salaj J, Obert B, Salaj T (eds) From gametes to embryos. In: Tenth international conference on plant embryology, Sept 5–8, 2001, Nitra, Slovak Republik, Book of Abstracts, p 107
• Preťová A, Williams EG (1986) Direct somatic embryogenesis from immature zygotic embryos of flax (Linum usitatissimum L.). J Plant Physiol 126:155–161
• Roger D, Gallusci P, Meyer Y, David A, David H (1998) Basic chitinases are correlated with the morphogenic response of flax cells. Physiol Plant 103:271–279
• Rojas-Herrera R, Loyola-Vargas VM (2002) Induction of a class III chitinase in foliar explants of Coffea arabica L during somatic embryogenesis and wounding. Plant Sci 163:705–711
• Salaj J, Petrovská B, Obert B, Preťová A (2005) Histological study of embryo-like structures initiated from hypocotyl segments of flax (Linum usitatissimum L.). Plant Cell Rep 24:590–595
• Salaj T, Blehova A, Salaj J (2007) Embryogenic suspension cultures of Pinus nigra Arn.: growth parameters and maturation ability. Acta Physiol. Plant 29:225–231
• Tchorbadjieva MI, Pantchev IY (2006) Secretion of a chitinase-like protein in embryogenic suspension cultures of Dactylis glomerata L. Biol Plant 50:142–145
• Tchorbadjieva MI, Pantchev IY, Haarizanova N (2004) Two-dimensional protein pattern analysis of extracellular proteins secreted by embryogenic and non-embryogenic suspension cultures of Dactylis glomerata L. Biotechnol Biotechnol Equip 18:20–27
• Tejavathi DH, Sita GL, Sunita AT (2000) Somatic embryogenesis in flax. Plant Cell Tissue Organ Cult 63:155–159
• Toonen MAJ, Schmidt EDL, van Kammen A, de Vries SC (1997) Promotive and inhibitory effects of diverse arabinogalactan proteins on Daucus carota L somatic embryogenesis. Planta 203:188–195
• van Hengel AJ (1998) Chitinases and arabinogalactan proteins in somatic embryogenesis. PhD Thesis, Wageningen, 120 pp
• van Hengel AJ, Tadesse Z, Immerzeel P, Schols H, van Kammen A, de Vries SC (2001) N-acetylglucosamin and glucosamine-containing arabinogalactan proteins control somatic embryogenesis. Plant Physiol 125:1880–1890
• Wendt dos Santos AL, Steiner R, Guerra MP, Zoglauer K, Moerschbacher BM (2008) Somatic embryogenesis in Araucaria angustifolia. Biol Plant 52:195–199