PL EN


Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2015 | 24 | 1 |

Tytuł artykułu

The effect of breed and feeding level on carcass composition, fatty acid profile and expression of genes encoding enzymes involved in fat metabolism in two muscles of pigs fed a diet enriched in n-3 fatty acids. A preliminary study

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
The experiment was performed according to a 2 x 2 factorial design with breed and level of nutrition as factors affecting fat metabolism in pigs. Two groups of gilts, each comprising 4 Polish Large White (PLW) and 4 Synthetic Line 990 (L990) animals, were fed from 60 to 105 kg body weight on the experimental diet at 85% or 95% of assumed ad libitum intake. The diet contained 2% of linseed, 0.5% rapeseed and 0.5% fish oils as the source of n-3 fatty acids (FA). The carcass protein content was smaller and backfat thickness greater in L990 than in PLW pigs. Also the intramuscular fat content in the biceps femoris (BF) and longissimus dorsi (LD) muscles and their contents of total FA, saturated fatty acids, monounsaturated fatty acids (MUFA) and polyunsaturated fatty acids (PUFA) were higher or tended to be higher in L990 than in PLW pigs, the differences between the breeds being more pronounced in the BF muscle. The n-6/n-3 PUFA ratio tended to be lower in the LD muscle, whereas in the BF muscle, it was lower in L990 than in PLW pigs. Feeding at the 85% level resulted in smaller backfat thickness and carcass fat content in animals of both breeds. Gene expression of stearoyl-CoA desaturase in both muscles was higher in L990 pigs, which could have resulted in the higher MUFA and PUFA contents in this breed. Gene expression of fatty acid-binding protein 4 and peroxisome proliferator-activated receptor were affected both by breed and feeding level only in the BF muscle.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

24

Numer

1

Opis fizyczny

p.31-40,fig.,ref.

Twórcy

autor
  • The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, 05-110 Jablonna, Poland
  • The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, 05-110 Jablonna, Poland
autor
  • The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, 05-110 Jablonna, Poland
autor
  • The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, 05-110 Jablonna, Poland
autor
  • The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, 05-110 Jablonna, Poland
autor
  • The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, 05-110 Jablonna, Poland

Bibliografia

  • AOAC, 2005. Association of Official Analytical Chemists, Official Methods of Analysis. 18th Edition. MA
  • Bessa R.J.B., Hughes R.A., Jeronimo E., Moreira O.C., Prates J.A.M., Doran O., 2013. Effect of pig breed and dietary protein level on selected fatty acids and stearoyl-coenzyme A desaturase protein expression in longissimus muscle and subcutaneous fat. J. Anim. Sci. 91, 4540–4546
  • Burkett J.L., 2009. The effect of selection for intramuscular fat on fatty acid composition in Duroc pigs. PhD Dissertation Ames, Iowa: Iowa State University, pp. 176. Available from: Iowa State University, lib.dr.iastate.edu/etd/10539 (accessed Apr 30, 2012)
  • Czarniecka-Skubina E., Przybylski W., Jaworska D., Wachowicz I., Urbańska I., Niemyjski S., 2007. Quality profile of pork meat with varying contents of intramuscular fat (in Polish). Food Science Technology Quality 6, 55, 285–294
  • Damon M., Louveau I., Lefaucheur L., Lebret B., Vincent A., Leroy P., Sanchez M.P., Herpin P., Gondret F., 2006. Number of intramuscular adipocytes and fatty acid binding protein-4 content are significant indicators of intramuscular fat level in crossbred Large White x Duroc pigs. J. Anim. Sci. 84, 1083–1092
  • Duran-Montgé P., Realini C.E., Barroeta A.C., Lizardo R.G., EsteveGarcia E., 2010. De novo fatty acid synthesis and balance of fatty acids of pigs fed different fat source. Livest. Sci. 132, 157–164
  • Folch J., Lees M., Stanley G.H.S., 1957. A simple method for the isolation and purification of lipids from animal tissues. J. Biol. Chem. 226, 497–509
  • Gao Y., Zhang Y.H., Jiang H. et al., 2011. Detection of differentially expressed genes in the longissimus dorsi of Northeastern Indigenous and Large White pigs. Genet. Mol. Res. 10, 779–791
  • Gerbens F., 2004. Genetic control of intramuscular fat accretion. In: M.F.W. te Pas, M.E. Everts, H.P. Haagsman (Editors). Muscle Development of Livestock Animals: Physiology, Genetics and Meat Quality. CAB International, Wallingford, OX (UK), chapter 16, pp. 343–362
  • Gerbens F., Jansen A., van Erp A.J., Harders F., Meuwissen T.H., Rettenberger G., Veerkamp J.H., te Pas M.F., 1998. The adipocyte fatty acid-binding protein locus: characterization and association with intramuscular fat content in pigs. Mamm. Genome 9, 12, 1022–1026
  • Guillevic M., Kouba M., Mourot J., 2009. Effect of a linseed diet or a sunflower diet on performances, fatty acid composition, lipogenic enzyme activities and stearoyl-CoA-desaturase activity in the pig. Livest. Sci. 124, 288–294
  • Kapelański W., Grajewska S., Bocian M., Urbański P., Jankowiak H., Roślewska A., Cebulska A., 2010. The effect of carcass fatness on the fatty acid profile in pig meat. In: W. Migdał, G. Cilev, B. Zivković, V. Jukna (Editors). Intramuscular Fat and Histological Structure of Meat, Modern Trends in Meat Production. Polskie Towarzystwo Technologów Żywności, Oddział Małopolska, Kraków (Poland), pp.41–46
  • Katsumata M., 2011. Promotion of intramuscular fat accumulation in porcine muscle by nutrition regulation. Anim. Sci. J. 82, 17–25
  • Kloareg M., Le Bellego L., Mourot J., Noblet J., van Milgen J., 2005. Deposition of dietary fatty acids and of de novo synthesised fatty acids in growing pigs: effects of high ambient temperature and feeding restriction. Brit. J. Nutr. 93, 803–811
  • Kloareg M., Noblet J., van Milgen J., 2007. Deposition of dietary fatty acids, de novo synthesis and anatomical partitioning of fatty acids in finishing pigs. Brit. J. Nutr. 97, 35–44
  • Kratz R., 2003. Einfluss unterschiedlicher Fettquellen in der Ernahrung von Schweinen unterschiedlicher Genetik auf den Protein- und Lipidansatz, das Fettsaurenmuster verschiedenerTeilstucke und die Fleischbeschaffenheit. PhD Dissertation, Gieβen (Germany): Justus-Liebig University, pp. 221. Available from: Justus-Liebig University, Gieβen, D-35392
  • McNeel R.L., Ding S.T., Smith E.O., Mersmann H.J., 2000. Effect of feed restriction on adipose tissue transcript concentrations in genetically lean and obese pigs. J. Anim. Sci. 78, 934–942
  • Pascual J.V., Rafecas M., Canela M.A., Boatella J., Bou R., Barroeta A.C., Codony R., 2007. Effect of increasing amounts of a linoleic-rich dietary fat on the fat composition of four pig breeds. Part II: Fatty acid composition in muscle and fat tissues. Food Chem. 100, 1639–1648
  • Pfaffl M.W., 2001. A new mathematical model for relative quantification in real time RT–PCR. Nucl. Acid. Res. 29, E45
  • Pfaffl M.W., Horgan G.W., Dempfle L., 2002. Relative Expression Software Tool (REST) for group-wise comparison and statistical analysis of relative expression results in real-time PCR. Nucl. Acid. Res. 30, E36
  • Raes K., De Smet K., Demeyer D., 2004. Effect of dietary fatty acids on incorporation of long chain polyunsaturated fatty acids and conjugated linoleic acid in lamb, beef and pork meat: a review. Anim. Feed Sci. Tech. 113, 199–221
  • Raj S., Poławska E., Skiba G., Weremko D., Fandrejewski H., Skomiał J., 2010. The influence of dietary source of fatty acids on chemical composition of the body and utilization of linoleic and linolenic acids by pigs. Anim. Sci. Pap. Rep. 28, 355–362
  • Realini C.E., Duran-Montgé P., Lizardo R., Gispert M., Oliver M.A., Esteve-Garcia E., 2010. Effect of source of dietary fat on pig performance, carcass characteristics and carcass fat content, distribution and fatty acid composition. Meat Sci. 85, 606–612
  • Skiba G., Raj S., Wojtasik M., Weremko D., 2012. Relationship between intake of PUFA n-3 fatty acids and their quantitative content in the carcass tissues of pigs. J. Anim. Feed Sci. 21, 648–660
  • Skiba G., Raj S., Poławska E., 2013. Profile of fatty acids and activity of elongase and ∆5 and ∆9 desaturase of growing pigs differ in concenytration of intramuscular fat in musculus longissimus dorsi. Anim. Sci. Pap. Rep. 31, 123–137
  • Szydlowski M., Buszka A., Mackowski M., Lechniak D., Switonski M., 2011. Polymorphism of genes encoding cytokines IL6 and TNF is associated with pig fatness. Livest. Sci. 136, 150–156
  • WHO/FAO, 2003. Diet Nutrition and the Prevention of Chronic Diseases. Report of a Joint WHO/FAO Expert Consultation. WHO Technical Report Series 916, Geneva
  • Wu T., Zhang Z., Yuan Z., Lo L.J., Chen J., Wang Y., Peng J., 2013. Distinctive genes determine different intramuscular fat and muscle fiber ratios of the longissimus dorsi muscles in Jinhua and landrace pigs. PLoS One [serial online], 8, 1, e53181. Available from www.plosone.org
  • Yu K., Shu G., Yuan F. et al., 2013. Fatty acid and transcriptome profiling of longissimus dorsi muscle between pig breeds differing in meat quality. Int. J. Biol. Sci. 9, 108–118

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

bwmeta1.element.agro-ac5fad00-1558-4d25-8f76-c1cdd627f8f9
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.