Relationship between pathogenicity, race and vegetative compatibility grouping among Algerian populations of Fusarium oxysporum f. sp. pisi causing pea wilt

• Autorzy: Merzoug A. , Belabid L.
• Języki publikacji: EN

Abstrakty

EN

Fusarium oxysporum f. sp. pisi (FOP) is a significant and destructive pathogen of field pea in Algeria. In the present study, 50 isolates of F. oxysporum f. sp. pisi, the causal agent of pea (Pisum sativum) wilt, collected from different parts of western Algeria and representing four races of the pathogen, were analyzed for virulence. The wilt incidence ranged from 6.66 to 88.33% on a highly susceptible cultivar (Little Marvel). Twenty-one isolates belonging to four races of FOP and one nonpathogenic F. oxysporum (FO) isolate were analyzed for vegetative compatibility in order to reveal the genetic structure of the population and to check the reliability of the method for the identification of physiological races of FOP. Obtained results showed that the FOP isolates could be classified into four main vegetative compatibility groups (VCGs) that corresponded to races l, 2A, 2B and 5. The race 6 isolate fell into the race 1 VCG. To our knowledge, this is the first such study in Algeria of its kind.

Słowa kluczowe

EN

relationship; pathogenicity; physiological race; vegetative compatibility group; Algerian population; Fusarium oxysporum f.sp.pisi; pea; Pisum sativum; wilt; pathogen

• Wydawca: -
• Czasopismo: Journal of Plant Protection Research
• Rocznik: 2017
• Tom: 57
• Numer: 4
• Opis fizyczny: p.370-378,fig.,ref.

Twórcy

autor

Merzoug A.

• Laboratory of Research on Biological Systems and Geomatics (LRSBG), Department of Agronomy, University of Mascara, Mascara, Algeria

autor

Belabid L.

• Laboratory of Research on Biological Systems and Geomatics (LRSBG), Department of Agronomy, University of Mascara, Mascara, Algeria

Bibliografia

• Abo K., Klein KK., Edel-Hermann V., Gautheron N., Dossahoua T., Steinberg C. 2005. High genetic diversity among strains of Fusarium oxysporum f. sp. vasinfectum from cotton in Ivory Coast. Phytopathology 95 (12): 1391–1396. DOI: https://doi.org/10.1094/phyto-95-1391
• Alves-Santos F.M., Martínez-Bermejo D., Rodríguez-Molina M.C., Díez J.J. 2007. Cultural characteristics, pathogenicity and genetic diversity of Fusarium oxysporum isolates from tobacco fields in Spain. Physiological and Molecular Plant Pathology 71 (1–3): 26–32. DOI: https://doi.o=rg/10.1016/j.pmpp.2007.09.007
• Alves-Santos F.M., Cordeiro-Rodrigues L., Sayagués J.M., Martín-Domínguez R., García-Benavides P., Crespo M.C., Díaz-Mínguez J.M., Eslava A.P. 2002. Pathogenicity and race characterization of Fusarium oxysporum f. sp. phaseoli isolates from Spain and Greece. Plant Pathology 51 (5): 605–611. DOI: https://doi.org/10.1046/j.1365-3059.2002.00745.x
• Baayen R.P., O’Donnell K., Bonants P.J.M., Cigelnik E., Kroon L.P.N.M., Roebroeck E.J.A., Waalwijk C. 2000. Gene genealogies and AFLP analyses in the Fusarium oxysporum complex identify monophyletic and nonmonophyletic formae speciales causing wilt and rot disease. Phytopathology 90 (8): 891–900. DOI: https://doi.org/10.1094/phyto.2000.90.8.891
• Baysal Ö., Siragusa M., Gümrükcü E., Zengin S., Carimi F., Sajeva M. 2010. Molecular characterization of Fusarium oxysporum f. sp. melongenae by ISSR and RAPD markers on eggplant. Biochemical Genetics 48 (5–6): 524–537. DOI:https://doi.org/10.1007/s10528-010-9336-1
• Bayraktar H.H., Turkkan M., Dolar F.S. 2010. Characterization of Fusarium oxysporum f. sp. cepae from onion in Turkey based on vegetative compatibility and rDNA RFLP analysis. Journal of Phytopathology 158 (10): 691–697. DOI:https://doi.org/10.1111/j.1439-0434.2010.01685.x
• Belabid L., Fortas Z. 2002. Virulence and vegetative compatibility of Algerian isolates of Fusarium oxysporum f. sp. lentis. Phytopathologia Mediterranea 41: 179–187.
• Bowden R.L., Leslie J.F. 1992. Nitrate-nonutilizing mutants of Gibberella zeae (Fusarium graminearum) and their use in determining vegetative compatibility. Experimental Mycology 16 (4): 308–315. DOI: https://doi.org/10.1016/0147-5975(92)90007-e
• Correll J.C., Klittich C.J.R., Leslie J.F. 1987. Nitrate nonutilising mutants of Fusarium oxysporum and the use in vegetative compatibility tests. Phytopathology 77 (12): 1640–1646.DOI: https://doi.org/10.1094/phyto-77-1640
• Correll J.C. 1991. The relationship between formoe speciales, races and vegetative compatibility groups in Fusarium oxysporum. The American Phytopathology Society 81 (9): 1061–1064.
• Cumagun J., Cumagun R., Zsachel O., Tolentino M., Relevante Ch., Balatero C. 2008. Vegetative compatibility among Fusarium oxysporum isolates from bitter gourd and bottle gourd in the Philippines. Journal of Plant Protection Research 48 (3): 283–293. DOI: https://doi.org/10.2478/v10045-008-0037-2
• Di Primo P., Cappelli C., Katan T. 2002. Vegetative compatibility grouping of Fusarium oxysporum f. sp. gladioli from saffron. European Journal of Plant Pathology 108 (9): 869–875. DOI: https://doi.org/10.1023/A:1021204022787
• Elena K., Pappas A.C. 2006. Race distribution, vegetative compatibility and pathogenicity of Fusarium oxysporum f. sp. melonis isolates in Greece. Journal of Phytopathology 154 (4): 250–255. DOI: https://doi.org/10.1111/j.1439-0434.2006.01099.x
• Haglund W.A., Kraft J.M. 2001. Fusarium wilt. p. 13–14. In: “Compendium of Pea Diseases and Pests” (Kraft J.M., Pfleger F.L., eds.). American Phytopathological Society, St. Paul, USA, 67 pp.
• Katan T., Katan J. 1999. Vegetative compatibility grouping in Fusarium oxysporum f. sp. radicis-lycopersici from the UK, the Netherlands, Belgium and France. Plant Pathology 48 (4): 541–549. DOI: https://doi.org/10.1046/j.1365-3059.1999.00362.x
• Klein K.K., Edel-Hermann V., Gautheron N., Traore D., Steinberg C. 2005. High genetic diversity among strains of Fusarium oxysporum f. sp. vasinfectum from cotton in Ivory Coast. Phytopathology 95 (12): 1391–1396. DOI: https://doi.org/10.1094/phyto-95-1391
• Leslie J.F. 1993. Fungal vegetative compatibility. Annual Review of Phytopathology 31 (1): 127–150. DOI: https://doi.org/10.1146/annurev.py.31.090193.001015
• Lori G., Edel-Hermann V., Gautheron N., Alabouvette C. 2004. Genetic diversity of pathogenic and nonpathogenic populations of Fusarium oxysporum isolated from carnation fields in Argentina. Phytopathology 94 (6): 661–668. DOI:https://doi.org/10.1094/phyto.2004.94.6.661
• Merzoug A., Belabid L., Youcef-Benkada M., Benfreha F., Bayaa B. 2014. Pea Fusarium wilt races in western Algeria. Plant Protection Sciences 50: 70–77.
• Mohammadi A., Mofrad N.N. 2009. Genetic diversity in population of Fusarium solani from cumin in Iran. Journal of Plant Protection Research 49 (3): 283–286. DOI: https://doi.org/10.2478/v10045-009-0045-x
• Mohammadi A., Nejad R.F., Mofrad N.N. 2012. Fusarium verticillioides from sugarcane, vegetative compatibility groups and pathogenicity. Plant Protection Science 48 (3): 80–84.
• Nagarajan G., Kang S.W., Nam M.H., Song J.Y., Yoo S.J., Kim H.G. 2006. Characterization of Fusarium oxysporum f. sp. fragariae based on vegetative compatibility group, random amplified polymorphic DNA and pathogenicity. Plant Pathology Journal 22 (3): 222–229. DOI: https://doi.org/10.5423/ppj.2006.22.3.222
• Nogales Moncada A.M., Jimenez Diaz M.R., Perez Artes E. 2009. Vegetative compatibility groups in Fusarium oxysporum f. sp. ciceris and F. oxysporum non-pathogenic to Chickpea. Journal of Phytopathology 15 (11–12): 729–735.DOI: https://doi.org/10.1111/j.1439-0434.2009.01562.x
• Okubara P.A., Schroeder K.L., Paulitz T.C. 2005. Real-time polymerase chain reaction: applications to studies on soilborne pathogens. Canadian Journal of Plant Pathology 27 (3): 300–313. DOI: http://dx.doi.org/10.1080/07060660509507229
• Pasquali M., Dematheis F., Gilardi G., Gullino M.L., Garibaldi A. 2005. Vegetative compatibility groups of Fusarium oxysporum f. sp. lactucae from lettuce. Plant Disease 89 (3): 237–240. DOI: https://doi.org/10.1094/pd-89-0237
• Puhalla J.E. 1985. Classification of strains of Fusarium oxysporum on the basis of vegetative compatibility. Canadian Journal of Botany 63 (2): 179–183. DOI: https://doi.org/10.1139/b85-020
• Rataj-Guranowska M. 2016. An efficient method for selecting stable tester strains of vegetative compatibility groups in Verticillium dahliae. Journal of Plant Protection Research 56 (2): 163–166. DOI: https://doi.org/10.1515/jppr-2016-0028
• Rodríguez-Molina C., Morales-Rodríguez C., Palo C., Osuna D., Iglesias J., García J.A. 2013. Pathogenicity, vegetative compatibility and RAPD analysis of Fusarium oxysporum isolates from tobacco fields in Extremadura. European Journal of Plant Pathology 136 (3): 639–650. DOI: https://doi.org/10.1007/s10658-013-0194-7
• Saabale P.R., Dubey S.C. 2014. Pathogenicity and vegetative compatibility grouping among Indian populations of Fusarium oxysporum f. sp. ciceris causing chickpea wilt. Phytoparasitica 42 (4): 465–473. DOI: https://doi.org/10.1007/s12600-014-0383-8
• Sharma P., Sharma K.D., Sharma R., Plaha P. 2006. Genetic variability in pea wilt pathogen Fusarium oxysporum f. sp. pisi in north western Himalayas. Indian Journal of Biotechnology (5): 298–302.
• Somrith A., Singburaudom N., Piasai O. 2011. Vegetative compatibility groups of Fusarium oxysporum f. sp. cubense. Kasetsart Journal – Natural Science 45: 451–460.
• Vakalounakis D.J., Wang Z., Fragkiadakis G.A., Skaracis G.N., Li D.B. 2004. Characterization of Fusarium oxysporum isolates obtained from cucumber in China by pathogenicity, VCG, and RAPD. Plant Disease 88 (6): 645–649. DOI:https://doi.org/10.1094/pdis.2004.88.6.645
• Whitehead D.S., Coddington A., Lewis B.G. 1992. Classification of races by DNA polymorphism analysis and vegetative compatibility grouping in Fusarium oxysporum f. sp. pisi. Physiological and Molecular Plant Pathology 41 (4): 295–305.DOI: https://doi.org/10.1016/0885-5765(92)90028-t
• Webb K.M., Case A.J., Brick M.A., Otto K., Schwartz H.F. 2013. Cross pathogenicity and vegetative compatibility of Fusarium oxysporum isolated from sugar beet. Plant Disease 97 (9): 1200–1206. DOI: http://dx.doi.org/10.1094/PDIS-11-12-1051-RE

Identyfikatory

DOI

10.1515/jppr-2017-0051