Orexins excites the neurons of the rat ventral lateral geniculate nucleus predominantly via OX2 receptors

• Autorzy: Palus K. , Chrobok L. , Lewandowski M.H.
• Języki publikacji: EN

Abstrakty

EN

BACKGROUND AND AIMS: Lateral geniculate nucleus (LGN) is the relay center for visual information received from the retina and consists of the dorsal (dLGN) and ventral lateral geniculate nucleus (vLGN) which are divided by intergeniculate leaflet (IGL) – the important element of the biological clock. The vLGN can be easily distinguished from the IGL based on the neuropeptide Y (NPY) immunoreactivity present only in the IGL. The vLGN is believed to control of the visuomotor functions being among the neuronal structures creating non-image forming visual system. One of the nonspecific brain projection that transmit non-photic information is orexinergic system. Orexins are involved in many behavioral states such as arousal, sleep, stress, and feeding. The aim of this study was to examine the direct effect of orexins on the spontaneous activity of vLGN neurons and the type of the orexin receptor present in the investigated structure. METHODS: Whole-cell patch clamp in vitro technique was used to record spontaneous activity of the vLGN neurons. All of the experiments were performed on the acute brain slices (250 µm) from 13–18 days old male Wistar rats. Orexins, TCS OX2 29 and SB 334867 were applied by bath perfusion. Immunofluorescent staining was performed to verify location of the neuron after each electrophysiological recording. RESULTS: Our data shows that orexins have a direct postsynaptic effect on the vLGN neurons. Orexin A and orexin B depolarized (6.26±1.07 mV, 6.75±1.69 mV, respectively). CONCLUSIONS: Our experiments with OX1 and OX2 receptors antagonists indicate predominance of OX2 receptor in the vLGN. To our best knowledge, there are the first data which shows depolarizing effect of orexins in the vLGN. Based on our data we can suppose that orexin system modulates this structure of non-image forming visual system. Further study is needed to evaluate the complex effect of orexins in the lateral geniculate complex. This work is supported by NSC grant OPUS V: 2013/09/B/ NZ4/00541.

• Wydawca: -
• Czasopismo: Acta Neurobiologiae Experimentalis
• Rocznik: 2015
• Tom: 75
• Numer: Supl.
• Opis fizyczny: p.S109

Twórcy

autor

Palus K.

• Department of Neurophysiology and Chronobiology, Jagiellonian University, Krakow, Poland

autor

Chrobok L.

• Department of Neurophysiology and Chronobiology, Jagiellonian University, Krakow, Poland

autor

Lewandowski M.H.

• Department of Neurophysiology and Chronobiology, Jagiellonian University, Krakow, Poland