A comparison of tuber slice and whole tuber tests for the assessment of potato resistance to tuber blight Phytophthora infestans (Mont.) de Bary

• Autorzy: Zarzycka H.
• Języki publikacji: EN

Abstrakty

EN

The resistance of potato tuber tissues to colonization by Phytophthora infestans was compared in 10 selected cultivars in two trials: on tuber slices and whole tubers with cut rose ends. When resistance to sporulation was used as the discriminating criterion, the selected cultivars segregated in a distinctly different order than when arranged according to other resistance components. Both the tuber slice test and the whole tuber test allowed resistance to sporulation to be assessed in the tested group of cultivars. The results obtained in both trials were reproducible and both criteria, i.e. the percentage of mycelium-covered area as well as sporulation intensity were equally useful for the assessment. No significant influence of the fungal growth though the tuber slice tissue was observed on the assessment of sporulation in comparison to the whole tuber test where the fungus grew directly on die wounded tissue after inoculadon. Assessment of the percentage of the area of the tuber slice exhibidng visible necrosis was found to be useless due to die lack of correlation with two criteria of resistance to colonization used in the whole tuber test, i.e. the rate and depth of penetration. The most useful parameter of the whole tuber test appeared to be assessment of the depth of penetration of the tuber tissues. This parameter showed the least variability and good correlation with other criteria used to evaluate resistance to colonizadon.

• Wydawca: -
• Czasopismo: Acta Agrobotanica
• Rocznik: 1990
• Tom: 43
• Numer: 1-2
• Opis fizyczny: p.95-107,fig.,ref.

Twórcy

autor

Zarzycka H.

• Institute for Potato Research, Milkow, 05-832 Rozalin, Poland

Bibliografia

• Bhatia S. K., Young R. J., 1985. Reaction of potato tuber slices to Phytophthora infestans in relation to physiological age. Am. Potato J. 62; 471-478.
• Bjor T., 1987. Testing the resistance of potato genotypes to tuber late blight. Potato Res. 30; 525-532.
• Black W., Mastenbroek C.. Mills W. R., Peterson L. C., 1953. A proposal for an international nomenclature of races of Phytophthora infestans and of genes controlling immunity in Solanum demissum derivatives. Euphytica 3: 173-240.
• Clarke D. D., Kassim M. Y. A., 1977. Resistance factors in potato tuber tissue to hyphal growth of Phytophthora infestans. Physiol. Plant Path. 11; 267-277.
• Darsow W., 1987. Long-term results of a tuber slice test for relative resistance to late blight. Potato Res. 30: 9-22.
• Deal K. L., Gallegly M E., Young R. J., 1974. Laboratory testing of potato tubers for multigenic resistance to late blight. Am. Potato J. 51: 324-329.
• Durska B., 1976. Wykorzystanie różnych metod w określaniu odporności bulw na zarazę ziemniaka (Phytophthora infestans (Mont) de Bary) na podstawie badań odmian ziemniaka zrejonizowanych w Polsce. Biul. Inst. Ziemn. 16: 19-37.
• Lacey J., 1967. Susceptibility of potato tubers to infection by Phytophthora infestans. Ann. Appl. Biol., 59: 257-264.
• Langton F. A., 1972. The development of a laboratory method, of assessing varietal resistance of potato tubers to late blight (Phytophthora infestans). Potato Res. 15: 290-301.
• Lapwood D. H., 1965. Laboratory assessments of the susceptibility of potato tuber tissue to blight (Phytophthora infestans). Europ. Potato J. 8: 215-229.
• Lapwood D. H., 1977. Factors affecting the field infection of potato tubers of different cultivars by blight (Phytophthora infestans). Ann. Appl. Biol. 85: 23-42.
• Pietkiewicz J., 1976. Charakterystyka odporności poziomej ziemniaka na zarazę (Phytophthora infestans (Mont) de Bary). Inst. Ziemn. Bonin, Rozprawa habilitacyjna, s. 66. SGGW-AR
• Pietkiewicz J., 1977. Zmienność reakcji bulw ziemniaka na (Phytophthora infestans (Mont) de Bary) w badaniach laboratoryjnych. Biul. Inst. Ziemn. 19: 49 60.
• Piotrowski W., Wojciechowska-Perz B., Pietkiewicz J.. 1973. Badania odporności bulw ziemniaka na zarazę ziemniaczaną (Phytophthora infestans (Mont) de Bary). Biul Inst. Ziemn. 11:45-59.
• Schober B., 1974. Methoden zur Testung von Kartoffelknollen auf Resistenz gegen Braunfaüle (Phytophthora infestans) und Schorf (Streptomyces scabies). Potato Res. 17: 354-355.
• Schober B., Hoppner E., 1972. Zur Methodic der Rezistenzprüfung von Kartoffelknollen gegen der Erreger der Braunfaüle (Phytophthora infestans (Mont) de Bary). Potato Res. 15: 378-383.
• Stewart M. E., McCalmont D. C., Wastie R. L., 1983. The effect of harvest date and the interval between harvest and inoculation on the assessment of the resistance of potato tubers to late blight. Potato Res. 26: 101-107.
• Wastie R. L., Caligari P.D.S., Stewart H., Mckay G. R., 1987. A glasshouse progeny test for resistance to tuber blight (Phytophthora infestans). Potato Res. 30: 533-538
• Zarzycka H., 1987. Problemy hodowli ziemniaka odpornego na Phytophthora infestans. Zesz. Probl. Post. Nauk Roln. 307: 341-347.
• Zarzycka H., 1988. Charakterystyka komponentów odporności bulw niektórych odmian oraz dzikich gatunków ziemniaka na Phytophthora infestans. Biul. Inst. Ziemn. (w druku).