Selection of reference genes for quantitative real-time RT-PCR on gene expression in Golden Pompano (Trachinotus ovatus)

• Autorzy: Chen X.J. , Zhang X.Q. , Huang S. , Cao Z.J. , Qin Q.W. , Hu W.T. , Sun Y. , Zhou Y.C.
• Języki publikacji: EN

Abstrakty

EN

Golden pompano (Trachinotus ovatus) is an important economically fish species. In this study, with an aim to identify reliable reference genes for quantitative real-time PCR (qRT-PCR) in golden pompano, we evaluated the expression stability of eight housekeeping genes in the presence and absence of poly I:C stimulation in eight tissues. The PCR data was analyzed by geNorm and NormFinder algorithms. The results showed that the expression of all the examined genes exhibited tissue-dependent variations. When under normal physiological condition, geNorm and NormFinder identified B2M and 18S as suitable genes. When studying gene expression under conditions of poly I:C stimulation, the selection of the internal controls should be selected on a tissue basis. At 12 h stimulation, geNorm ranked Actin/UBCE, Actin/B2M, UBCE/B2M, Actin/UBCE, RPL13/B2M, UBCE/GAPDH, B2M/RPL13, and UBCE/B2M, respectively, as the most stably expressed genes in liver, spleen, kidney, gill, intestine, heart, muscle, and brain. Comparable ranking orders were produced by NormFinder. Similar results were obtained at 48 h stimulation. Taken together, these results indicate that B2M and 18S are the most stable gene across tissue types under normal physiological conditions. However, during poly I:C stimulation, no single gene or single pair of genes in the examined set of housekeeping genes can serve as a universal reference across all tissue types. If one gene is preferred, B2M, B2M, UBCE, Actin, B2M/RPL13, B2M, B2M, and RPL13 may be used in spleen, kidney, liver, gill, intestine, brain, muscle, and heart of golden pompano, respectively.

• Wydawca: -
• Czasopismo: Polish Journal of Veterinary Sciences
• Rocznik: 2017
• Tom: 20
• Numer: 3
• Opis fizyczny: p.583-594,fig.,ref.

Twórcy

autor

Chen X.J.

• State Key Laboratory of Marine Resource Utilization in South China Sea, Hainan University, P.R. China

autor

Zhang X.Q.

• College of Marine Sciences, Hainan University, 58 Renmin Avenue, Haikou, P. R. China

autor

Huang S.

• College of Marine Sciences, Hainan University, 58 Renmin Avenue, Haikou, P. R. China

autor

Cao Z.J.

• College of Marine Sciences, Hainan University, 58 Renmin Avenue, Haikou, P. R. China

autor

Qin Q.W.

• College of Marine Science, South China Agricultural University, Guangzhou 510642, China

autor

Hu W.T.

• College of Marine Sciences, Hainan University, 58 Renmin Avenue, Haikou, P. R. China

autor

Sun Y.

• State Key Laboratory of Marine Resource Utilization in South China Sea, Hainan University, P.R. China
• College of Marine Sciences, Hainan University, 58 Renmin Avenue, Haikou, P. R. China

autor

Zhou Y.C.

• State Key Laboratory of Marine Resource Utilization in South China Sea, Hainan University, P.R. China

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Identyfikatory

DOI

10.1515/pjvs-2017-0071