The influance of CRF and alpha-hellical CRF(9-41) on rat fear responses and amino acids concentration in the central nucleus of amygdala

• Autorzy: Skorzewska A. , Bidzinski A. , Hamed A. , Lehner M. , Turzynska D. , Sobolewska A. , Szyndler J. , Maciejak P.
• Języki publikacji: EN

Abstrakty

EN

In the previous study we showed that exogenous CRF (1 μg/rat, icv) enhanced rat-freezing responses and increased expression of CRFrelated immunoreactive complexes in the amygdala. On the other hand, a non-selective CRF receptor antagonist, α-helical CRF(9-41) (10 μg/rat, i.c.v.) decreased rat fear response and increased the concentration of GABA in the amygdala (in vitro). The aim of the present study was to examine the effects of CRF and α-helical CRF(9-41), on the freezing reaction in the conditioned fear test and the release of amino acids in the central nucleus of amygdala (CeA), using microdialysis technique. It was found that CRF increased the concentration of aspartate, glutamate and Glu/GABA ratio in the CeA, an effect that preceded an increased expression of anxiety-like responses, which appeared 15 min after drug administration. α-helical CRF(9-41), decreased rat freezing responses and increased the local concentration of GABA, during the fi rst 30 min of observation. The present data show an important role of CRF in the central nucleus of the amygdala in the integration of anxiety-related, biochemical and behavioural responses, and suggest an involvement of amino acids innervation of the CeA, in the effects of this neurohormone.

• Wydawca: -
• Czasopismo: Acta Neurobiologiae Experimentalis
• Rocznik: 2009
• Tom: 69
• Numer: 3
• Opis fizyczny: p.361

Twórcy

autor

Skorzewska A.

• Department of Neurochemistry, Institute of Psychiatry and Neurology, Warsaw, Poland

autor

Bidzinski A.

• Department of Neurochemistry, Institute of Psychiatry and Neurology, Warsaw, Poland

autor

Hamed A.

• Department of Experimental and Clinical Pharmacology, Medical University of Warsaw, Warsaw, Poland

autor

Lehner M.

• Department of Neurochemistry, Institute of Psychiatry and Neurology, Warsaw, Poland

autor

Turzynska D.

• Department of Neurochemistry, Institute of Psychiatry and Neurology, Warsaw, Poland

autor

Sobolewska A.

• Department of Neurochemistry, Institute of Psychiatry and Neurology, Warsaw, Poland

autor

Szyndler J.

• Department of Neurochemistry, Institute of Psychiatry and Neurology, Warsaw, Poland

autor

Maciejak P.

• Department of Neurochemistry, Institute of Psychiatry and Neurology, Warsaw, Poland
• Department of Experimental and Clinical Pharmacology, Medical University of Warsaw, Warsaw, Poland