Expression of the zga agglutinin gene in tobacco can enhance its anti-pest ability for peach-potato aphid (Myzus persica)

• Autorzy: Zhou C. , Qian Z. , Ji Q. , Xu H. , Chen L. , Luo X. , Min L. , Tang K. , Xiao J. , Kai G.
• Języki publikacji: EN

Abstrakty

EN

The effect of introduction of the Zephyranthes grandiflora agglutinin gene (zga) to tobacco on its anti-pest ability for peach-potato aphids was investigated. PCR analysis confirmed that the zga gene was integrated into the plant genome. The results from semi-quantitative RT-PCR and real-time PCR assays revealed that the zga gene was expressed at various levels in the transgenic plants. A bioassay with aphids indicated that transgenic plants conferred enhanced resistance to aphids. Compared with the controls, the average number of aphids fed with transgenic plants during a 20-day assay evidently decreased by 70.4% in leaf disc bioassay and 77.9% in whole plant bioassay. The average number of nymph was significantly reduced by 36.4% on zga-expressing plants in leaf disc bioassay and 35.6% in whole plant bioassay. The report indicated that the introduction of zga gene to tobacco plants is a useful method to improve its anti-pest ability for aphids.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2011
• Tom: 33
• Numer: 5
• Opis fizyczny: p.2003-2010,fig.,ref.

Twórcy

autor

Zhou C.

• Laboratory of Plant Biotechnology, College of Life and Environment Sciences, Shanghai Normal University, Shanghai, 200234, People s Republic of China

autor

Qian Z.

• Laboratory of Plant Biotechnology, College of Life and Environment Sciences, Shanghai Normal University, Shanghai, 200234, People s Republic of China

autor

Ji Q.

• Laboratory of Plant Biotechnology, College of Life and Environment Sciences, Shanghai Normal University, Shanghai, 200234, People s Republic of China

autor

Xu H.

• Laboratory of Plant Biotechnology, College of Life and Environment Sciences, Shanghai Normal University, Shanghai, 200234, People s Republic of China

autor

Chen L.

• Laboratory of Plant Biotechnology, College of Life and Environment Sciences, Shanghai Normal University, Shanghai, 200234, People s Republic of China

autor

Luo X.

• Laboratory of Plant Biotechnology, College of Life and Environment Sciences, Shanghai Normal University, Shanghai, 200234, People s Republic of China

autor

Min L.

• Laboratory of Plant Biotechnology, College of Life and Environment Sciences, Shanghai Normal University, Shanghai, 200234, People s Republic of China

autor

Tang K.

• Plant Biotechnology Research Center, Fudan-SJTU-Nottingham Plant Biotechnology R&D Center, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 200030, People s Republic of China

autor

Xiao J.

• Laboratory of Plant Biotechnology, College of Life and Environment Sciences, Shanghai Normal University, Shanghai, 200234, People s Republic of China

autor

Kai G.

• Laboratory of Plant Biotechnology, College of Life and Environment Sciences, Shanghai Normal University, Shanghai, 200234, People s Republic of China

Bibliografia

• Barre A, Van Damme EJM, Peumans WJ, Rouge P (1996) Structure–function relationship of monocot mannose binding lectins. Plant Physiol 112:1531–1540
• Barry BD, Darrah LL, Huckla DL, Antonio AQ, Smith GS, O’Day MH (2000) Performance of transgenic corn hybrids in Missouri for insect control and yield. J Econ Entomol 93:993–999
• Braendle C, Davis GK, Brisson JA, Sten DL (2006) Wing dimorphism in aphids. Heredity 97:192–197
• Chang T, Chen L, Chen S, Cai H, Liu X, Xiao G, Zhu Z (2003) Transformation of tobacco with genes encoding Helianthus tuberosus agglutinin (HTA) confers resistance to peach-potato aphid (Myzus persicae). Transgenic Res 12(5):607–614
• Cheng X, Sardana R, Kaplan H, Altosaar I (1998) Agrobacteriumtransformed rice plants expressing synthetic cryIA(b) and cryIA(c) genes are highly toxic to striped stem borer and yellow stem borer. Proc Natl Acad Sci USA 95:2767–2772
• Datta K, Vasquez Z, Tu J, Torrizo L, Alam MF, Oliva N et al (1998) Constitutive and tissue-specific differential expression of the cryIA(b) gene in transgenic rice plants conferring resistance to rice insect pests. Theor Appl Genet 97:20–30
• Edwards K, Johnston C, Thompson C (1991) A simple and rapid method for the preparation of plant genomic DNA for PCR analyses. Nucl Acids Res 98:1349
• Foissac X, Loc NT, Christou P, Gatehouse AMR, Gatehouse JA (2000) Resistance to green leafhopper (Nephotettix virescens) and brown planthopper (Nilaparvata lugens) in transgenic rice expressing snowdrop lectin (Galanthus nivalis agglutinin; GNA). J. Insect Physiol 46:573–583
• Gatehouse AMR, Down RE, Powell KS, Sauvion N, Newell CA, Rahbé Y, Merryweather A, Hamilton WDO, Gatehouse JA (1996) Transgenic potato plants with enhanced-resistance to the peachpotato aphid Myzus persicae. Entomol Exp Appl 79:295–307
• Hilder VA, Powell KS, Gatehouse AMR, Gatehouse JA, Gatehouse LN, Shi Y, Hamilton WDO, Merryweather A, Newell CA, Tians JC, Peumans WJ, van Damme E, Boulter D (1995) Expression of snowdrop lectin in transgenic tobacco plants results in added protection against aphid. Transgenic Res 4(1):18–25
• Kai GY, Zheng JU, Zhang L, Pang YZ, Liao ZH, Li ZG, Zhao LX, Sun XF, Tang KX (2003) Molecular cloning of a new lectin gene from Z grandiflora. DNA Seq 14:335–338
• Li HF, Li XG, Liu X, Chang TJ, Xiao GF, Zhu Z (2002) Maize transformation of cryIAc3 gene and insect resistance of their transgenic plants. Acta Bot Sin 44:684–688
• Maddock SE, Hufman G (1991) Expressing in maize plants of wheat germ agglutinin, a novel source of insect resistance. In: Proceedings of the 3rd Int Cong Plant Mol Biol, Tucson, Arizona
• Mochida O, Wahyu A, Surjani TK (1979) Some considerations on screening resistant cultivars/lines of rice plant to the brown planthopper. Nilparvata lugens (Stal) (Hom, Delphacidae). IRRI, Los Banos, Philippines, pp 1–9
• Müler CB, Williams I, Hardeie J (2001) The role of nutrition, crowding and interspecific interactions in the development of winged aphids. Ecol Entomol 26:330–340
• Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497
• Nagadhara D, Ramesh S, Pasalu IC, Kondala RY, Krishnaiah NV, Sarma NP, Bown DP, Gatehouse JA, Reddy VD, Rao KV (2003) Transgenic indica rice resistant to sap-sucking insects. Plant Biotechnol J 1(3):231–240
• Perlak FJ, Oppenhuizen M, Gustafson K, Voth R, Sivasupramaniam S, Heering D et al (2001) Development and commercial use of Bollgard, cotton in the USA—early promises versus today’s reality. Plant J 27:489–501
• Peumans WJ, Van Damme EJ M (1995) Lectins as plant defense proteins. Plant Physiol 109:347–352
• Rahbe Y, Sauvion N, Febvay G, Peumans WJ, Gatehouse AMR (1995) Toxicity of lectins and processing of ingested proteins in the pea aphid Acyrthosiphon pisum. Entomol Exp Appl 76:143–155
• Renckens S, De GH, Van MM, Hernalsteens JP (1992) Petunia plants escape from negative selection against a transgene by silencing the foreign DNA via methylation. Mol Gen Genet 233:53–64
• Sauvion N, Rahbe Y, Van Damme EJ M, Gatehouse JA, Gatehouse AMR (1996) Effects of GNA and other mannose binding lectins on development and fecundity of the peach-potato aphids. Entomol Exp Appl 79:285–293
• Shukle RH, Murdock LL (1983) Lipoxygenase, trypsin inhibition, and lectin from soybeans: effect on larval growth of Manduca sexta (Lepidoptera: Sphingidae). Environ Entomol 12:787–791
• Stoger E, Williams S, Christou P, Down RE, Gatehouse JA (1999) Expression of the insecticidal lectin from snowdrop (Galanthus nivalis agglutinin; GNA) in transgenic wheat plants: effects on predation by the grain aphid Sitobion avenae. Mol Breed 5:65–73
• Sun X, Wu A, Tang K (2002) Transgenic rice lines with enhanced resistance to the small brown planthopper. Crop Prot 21:511–514
• Yao JH, Zhao XY, Liao ZH, Lin J, Chen ZH, Chen F, Song J, Sun XF, Tang KX (2003) Cloning and molecular characterization of a novel lectin gene from Pinellia ternata. Cell Res 13(4):301–308
• Ye SH, Chen S, Zhang F, Wang W, Tian Q, Liu JZ, Chen F, Bao JK (2009) Transgenic tobacco expressing Zephyranthes grandiflora agglutinin confers enhanced resistance to aphids. Appl Biochem Biotechnol 158(3):615–630
• Yuan ZQ, Zhao CY, Zhou Y, Tian YC (2001) Modification of GNA gene and studies on its aphid resistant. Acta Bot Sin 43(6):592–597

Uwagi

PL

Rekord w opracowaniu