Validation of real-time PCR methods for the quantification of genetically-modified maize and soybean

• Autorzy: Sieradzki Z. , Kwiatek K.
• Języki publikacji: EN

Abstrakty

EN

The objective of this study was to validate TaqMan real-time PCR procedures for the quantification of genetically-modified maize (events: MON810, Bt11, Bt176, and T25) and soybean (event: GTS 40-3-2) with the capillary LightCycler 2.0 instrument. The quantification was done by the amplification of reference maize genes: hmg (MON810, Bt11), zSSIIb (Bt176), and adh1 (T25), and genetically-modified DNA fragments containing a part of the maize genome and modified genes at 5' end (MON810, Bt11, T25), or unique DNA sequence of genes (cryIAb/IVS9) used for the modification (Bt176). As regards GM soybean event GTS 40-3-2, soya specific lectin gene and GM construct-specific DNA fragment (CaMV35S/ CTP- EPSPS) were amplified in real-time PCR reactions. Real-time PCR performance criteria achieved in this validation study proved that it is possible to use the reaction protocol made for 96-well plates real-time PCR instruments at capillary Roche thermocycler. Result of the performance criteria obtained for real-time PCR validation study for the quantification of genetically-modified maize or soybean were satisfactory, close or ever better than the values of inter-laboratory ring-trials.

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2009
• Tom: 53
• Numer: 4
• Opis fizyczny: p.740-746,ref.

Twórcy

autor

Sieradzki Z.

• Department of Hygiene of Animal Feedingstuffs, National Veterinary Research Institute, 24-100 Pulawy, Poland

autor

Kwiatek K.

• Department of Hygiene of Animal Feedingstuffs, National Veterinary Research Institute, 24-100 Pulawy, Poland

Bibliografia

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