Assessment of the role of meta-topolins on in vitro produced phenolics and acclimatization cometence of micropropagated 'Williams' banana

• Autorzy: Aremu A.O. , Bairu M. W. , Szucova L. , Dolezal K. , Finnie J. F. , Van Staden J.
• Języki publikacji: EN

Abstrakty

The effects of five topolins (meta-Topolin, mT; meta-Topolin riboside, mTR; meta-Methoxy topolin, MemT; meta-Methoxy topolin riboside, MemTR and meta-Methoxy topolin 9-tetrahydropyran-2-yl, MemTTHP) on the phenolic content and subsequent acclimatization potential of micropropagated ‘Williams’ bananas were compared to benzyladenine (BA). Sterile shoot-tip explants were cultured on modified Murashige and Skoog (MS) media containing 10, 20 or 30 µM of the above aromatic cytokinins (CKs) for 42 days. Phenolic contents were quantified spectrophotometrically. Growth parameters and photosynthetic pigments of the greenhouse-acclimatized plants were determined after 5 months. Total phenolic levels were highest in 10 µM mTtreated plantlets within the aerial parts and 30 µMMemTTHP for the underground parts. In the underground parts, 10 µM mT resulted in the production of the highest amount of proanthocyanidins which was approximately five-fold higher than in the control plants. Furthermore, 10 µMMemTTHPtreated plantlets had significantly higher total flavonoids (30.1 ± 0.24 mg CE/g DW) within the aerial parts. Plantlets regenerated using MemT, MemTR and MemTTHP had significantly longer roots and better shoot/root ratios compared to the control and BA-treated plants. In terms of root fresh weight, it was significantly higher in MemT-treated plantlets than in the control and BA treatments. Chlorophyll a/b ratio was significantly improved with the use of MemT, mTR and mT compared to control. Current findings indicate the potential of topolins in stimulating the accumulation of phenolic compounds in micropropagated plantlets. In view of the importance of plant secondary metabolites, their substantial accumulation probably enhanced the acclimatization and subsequent ex vitro survival of the micropropagated plantlets. Topolins, particularly, the new derivative MemTTHP could be an alternative CK for the micropropagation of plant species based on their stimulatory effect on ex vitro rooting that inevitably enhances acclimatization competence. Furthermore, topolins are demonstrated as potential elicitors in micropropagation.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2012
• Tom: 34
• Numer: 6
• Opis fizyczny: p.2265-2273,fig.,ref.

Twórcy

autor

Aremu A.O.

• Research Centre for Plant Growth and Development, School of Life Sciences, University of KwaZulu-Natal Pietermaritzburg, Private Bag X01, 3209 Scottsville, South Africa

autor

Bairu M. W.

• Research Centre for Plant Growth and Development, School of Life Sciences, University of KwaZulu-Natal Pietermaritzburg, Private Bag X01, 3209 Scottsville, South Africa

autor

Szucova L.

• Laboratory of Growth Regulators, Palacky University and Institute of Experimental Botany AS CR, Slechtitelu 11, 783 71 Olomouc, Czech Republic
• Centre of the Regional Hana for Biotechnological and Agricultural Research, Faculty of Science, Palacky University, Slechtitelu 11, 783 71 Olomouc, Czech Republic

autor

Dolezal K.

• Laboratory of Growth Regulators, Palacky University and Institute of Experimental Botany AS CR, Slechtitelu 11, 783 71 Olomouc, Czech Republic
• Centre of the Regional Hana for Biotechnological and Agricultural Research, Faculty of Science, Palacky University, Slechtitelu 11, 783 71 Olomouc, Czech Republic

autor

Finnie J. F.

• Research Centre for Plant Growth and Development, School of Life Sciences, University of KwaZulu-Natal Pietermaritzburg, Private Bag X01, 3209 Scottsville, South Africa

autor

Van Staden J.

• Research Centre for Plant Growth and Development, School of Life Sciences, University of KwaZulu-Natal Pietermaritzburg, Private Bag X01, 3209 Scottsville, South Africa

Bibliografia

• Adedipe NO, Hunt LA, Fletcher RA (1971) Effects of benzyladenine on photosynthesis, growth and senescence of the bean plant. Physiol Plant 25:151–153
• Aremu AO, Bairu MW, Doležal K, Finnie JF, Van Staden J (2012) Topolins: a panacea to plant tissue culture challenges? Plant Cell Tissue Organ Cult 108:1–16
• Bairu MW, Stirk WA, Doležal K, Van Staden J (2007) Optimizing the micropropagation protocol for the endangered Aloe polyphylla: can meta-topolin and its derivatives serve as replacement for benzyladenine and zeatin? Plant Cell Tissue Organ Cult 90:15–23
• Bairu MW, Stirk WA, Doležal K, Van Staden J (2008) The role of topolins in micropropagation and somaclonal variation of banana cultivars ‘Williams’ and ‘Grand Naine’ (Musa spp. AAA). Plant Cell Tissue Organ Cult 95:373–379
• Bairu MW, Aremu AO, Van Staden J (2011a) Somaclonal variation in plants: causes and detection methods. Plant Growth Regul 63:147–173
• Bairu MW, Novák O, Doležal K, Van Staden J (2011b) Changes in endogenous cytokinin profiles in micropropagated Harpagophytum procumbens in relation to shoot-tip necrosis and cytokinin treatments. Plant Growth Regul 63:105–114
• Bari R, Jones JDG (2009) Role of plant hormones in plant defence responses. Plant Mol Biol 69:473–488
• Baroja-Fernández E, Aguirreolea J, Martínková H, Hanus J, Strnad M (2002) Aromatic cytokinins in micropropagated potato plants. Plant Physiol Biochem 40:217–224
• Bernier PY, Lamhamedi MS, Simpson DG (1995) Shoot:root ratio is of limited use in evaluating the quality of container conifer stock. Tree Planters Notes 46:102–106
• Binks RH, Greenham JR, Luis JG, Gowen SR (1997) A phytoalexin from roots of Musa acuminata var. Pisang sipulu. Phytochemistry 45:47–49
• Buer CS, Imin N, Djordjevic MA (2010) Flavonoids: new roles for old molecules. J Integrat Plant Biol 52:98–111
• Caponetti JD, Gray DJ, Trigiano RN (2005) History of plant tissue and cell culture. In: Trigiano RN, Gray DJ (eds) Plant development and biotechnology. CRC Press, Florida, pp 9–15
• Čatský J, Pospíšilová J, Kamínek M, Gaudinová A, Pulkrábek J, Zahradníček J (1996) Seasonal changes in sugar beet photosynthesis as affected by exogenous cytokinin N⁶-(m-hydroxybenzyl) adenosine. Biol Plant 38:511–518
• Chow WS, Anderson JM, Melis A (1990) The photosystem stoichiometry in thylakoids of some Australian shade-adapted plant species. Aust J Plant Physiol 17:665–674
• Collingborn FMB, Gowen SR, Mueller-Harvey I (2000) Investigations into the biochemical basis for nematode resistance in roots of three Musa cultivars in response to Radopholus similis infection. J Agric Food Chem 48:5297–5301
• Criado M, Roberts I, Echeverria M, Barneix A (2007) Plant growth regulators and induction of leaf senescence in nitrogen-deprived wheat plants. J Plant Growth Regul 26:301–307
• Curir P, VanSumere CF, Termini A, Barthe P, Marchesini A, Dolci M (1990) Flavonoid accumulation is correlated with adventitious roots formation in Eucalyptus gunnii Hook micropropagated through axillary bud stimulation. Plant Physiol 92:1148–1153
• De Klerk G-J, Guan H, Huisman P, Marinova S (2011) Effects of phenolic compounds on adventitious root formation and oxidative decarboxylation of applied indoleacetic acid in Malus ‘Jork 9’. Plant Growth Regul 63:175–185
• Dixon RA, Paiva NL (1995) Stress-induced phenylpropanoid metabolism. Plant Cell 7:1085–1097
• Escalona M, Cejas I, González-Olmedo J, Capote I, Roels S, Cañal MJ, Rodríguez R, Sandoval J, Debergh P (2003) The effect of meta-topolin on plantain propagation using a temporary immersion bioreactor. InfoMusa 12:28–30
• Genkov T, Tsoneva P, Ivanova I (1997) Effect of cytokinins on photosynthetic pigments and chlorophyllase activity in in vitro cultures of axillary buds of Dianthus caryophyllus L. J Plant Growth Regul 16:169–172
• Jain SM (2004) Introduction: cellular biology and biotechnology including mutation techniques for creation of new useful banana genotypes. In: Jain SM, Swennen R (eds) Banana improvement: cellular, molecular biology, and induced mutations. Science Publishers, Inc., Enfield, pp xv–xvi
• Lewinsohn E, Gijzen M (2009) Phytochemical diversity: the sounds of silent metabolism. Plant Sci 176:161–169
• Lichtenthaler HK (1987) Chlorophylls and carotenoids: pigments of photosynthetic biomembranes. In: Douce R, Packer L (eds) Methods in enzymology, vol 148., Academic Press New York, USA, pp 350–382
• Liu X-N, Zhang X-Q, Sun J-S (2007) Effects of cytokinins and elicitors on the production of hypericins and hyperforin metabolites in Hypericum sampsonii and Hypericum perforatum. Plant Growth Regul 53:207–214
• Makkar HPS, Sidhuraju P, Becker K (2007) Plant secondary metabolites. Humana Press Inc., New Jersey
• Marinova D, Ribarova F, Atanassova M (2005) Total phenolics and total flavonoids in Bulgarian fruits and vegetables. J Univ Cheml Technol Metal 40:255–260
• Murashige T, Skoog F (1962) A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plant 15:473–497
• Mutui TM, Mibus H, Serek M (2012) Effect of meta-topolin on leaf senescence and rooting in Pelargonium × hortorum cuttings. Postharvest Biol Technol 63:107–110
• Namdeo AG (2007) Plant cell elicitation for production of secondary metabolites: a review. Pharmacogn Rev 1:69–79
• Palavan-Ünsal N, Çağ S, Çetin E (2004) The role of meta-topolin in senescence of wheat leaf segments. J Cell Mol Biol 3:23–31
• Peer WA, Murphy AS (2007) Flavonoids and auxin transport: modulators or regulators? Trends Plant Sci 12:556–563
• Porter LJ, Hrstich LN, Chan BG (1985) The conversion of proanthocyanidins and prodelphinidins to cyanidin and delphinidin. Phytochemistry 25:223–230
• Pospíšilová J, Synková H, Haisel D, Čatský J, Wilhelmová N, Šrámek F (1999) Effect of elevated CO₂ concentration on acclimation of tobacco plantlets to ex vitro conditions. J Exp Bot 50:119–126
• Pospíšilová J, Synková H, Haisel D, Semorádová S (2007) Acclimation of plantlets to ex vitro conditions: effects of air humidity, irradiance, CO₂ concentration and abscisic acid (a review). Acta Hortic 748:29–38
• Quiala E, Cañal M-J, Meijón M, Rodríguez R, Chávez M, Valledor L, de Feria M, Barbón R (2012) Morphological and physiological responses of proliferating shoots of teak to temporary immersion and BA treatments. Plant Cell Tissue Organ Cult. 109:223–234
• Ramachandra Rao S, Ravishankar GA (2002) Plant cell cultures: chemical factories of secondary metabolites. Biotechnol Adv 20:101–153
• Szücová L, Spíchal L, Dolezal K, Zatloukal M, Greplová J, Galuszka P, Krystof V, Voller J, Popa I, Massino FJ, Jørgensen J-E, Strnad M (2009) Synthesis, characterization and biological activity of ring-substituted 6-benzylamino-9-tetrahydropyran-2-yl and 9-tetrahydrofuran-2-ylpurine derivatives. Bioorg Med Chem 17:1938–1947
• Valero-Aracama C, Kane M, Wilson S, Vu J, Anderson J, Philman N (2006) Photosynthetic and carbohydrate status of easy-and difficult-to-acclimatize sea oats (Uniola paniculata L.) genotypes during in vitro culture and ex vitro acclimatization. In Vitro Cell Dev Biol Plant 42:572–583
• Valero-Aracama C, Kane M, Wilson S, Philman N (2010) Substitution of benzyladenine with meta-topolin during shoot multiplication increases acclimatization of difficult- and easy-to-acclimatize sea oats (Uniola paniculata L.) genotypes. Plant Growth Regul 60:43–49
• Van Staden J, Cook EL, Noodén LD (1988) Cytokinins and senescence. In: Noodén LD, Leopold AC (eds) Senescence and aging in plants. Academic Press, Inc., San Diego, pp 281–328
• Vogt T (2010) Phenylpropanoid biosynthesis. Mol Plant 3:2–20
• Vuylsteke DR (1998) Shoot-tip culture for the propagation, conservation, and distribution of Musa germplasm. International Institute of Tropical Agriculture, Ibadan
• Werbrouck SPO, Van der Jeugt B, Dewitte W, Prinsen E, Van Onckelen HA, Debergh PC (1995) The metabolism of benzyladenine in Spathiphyllum floribundum ‘Schott Petite’ in relation to acclimatisation problems. Plant Cell Rep 14:662–665
• Werbrouck SPO, Strnad M, Van Onckelen HA, Debergh PC (1996) Meta-topolin, an alternative to benzyladenine in tissue culture? Physiol Plant 98:291–297
• Werner T, Motyka V, Strnad M, Schmülling T (2001) Regulation of plant growth by cytokinin. Proc Natl Acad Sci 98:10487–10492
• Wu HC, du Toit ES, Reinhardt CF, Rimando AM, van der Kooy F, Meyer JJM (2007) The phenolic, 3,4-dihydroxybenzoic acid, is an endogenous regulator of rooting in Protea cynaroides. Plant Growth Regul 52:207–215
• Wuyts N, Lognay G, Sági L, De Waele D, Swennen R (2005) Secondary metabolites in roots and implications for nematode resistance in banana (Musa spp.). In: Turner DW, Rosales FE (eds) Banana root system: towards a better understanding for its productive management. Proceedings of an international symposium. The International Network for the Improvement of Banana and Plantain, Montpellier, pp 238–246
• Wuyts N, Lognay G, Verscheure M, Marlier M, De Waele D, Swennen R (2007) Potential physical and chemical barriers to infection by the burrowing nematode Radopholus similis in roots of susceptible and resistant banana (Musa spp.). Plant Pathol 56:878–890
• Yokoyama M, Naito K, Suzuki H (1980) Effects of benzyladenine on chlorophyll, DNA, RNA and protein content of attached young bean (Phaseolus vulgaris L.) leaves. Ann Bot 45:649–653
• Zhishen J, Mengcheng T, Jianming W (1999) The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chem 64:555–559

Uwagi

Rekord w opracowaniu