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2014 | 36 | 01 |

Tytuł artykułu

Gene expression in opening and senescing petals of rose (Rosa hybrida L.)

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
Roses are one of the most important cut flowers which are available in more color and sizes throughout the year. Senescence is a programmed event responding to external (environmental) and internal (developmental) signals; it requires de novo gene expression and protein synthesis, and is controlled in a highly coordinated manner. In order to know about molecular mechanisms and complex nets involved in aging regulating pathways, a cDNA-amplified fragment length polymorphism (cDNA-AFLP) analysis was performed to identify and isolate senescence-associated genes (SAGs) in rose petals of two cultivars ‘Black magic’ and ‘Marroussia’ with different longevities. The transcription profiles of plants at different developmental stages (flowering and senescent) were compared. About 1,600 cDNA fragments, ranging in size from 250 to 1,200 bp, were reproducibly detected. This allowed the identification of 22 differentially expressed cDNAs corresponding to genes belonging to different functional categories related to senescence, signal transduction pathway, carbohydrate metabolism, energy and other catabolic events such as protein, lipid and cell wall degradation. SAGs-encoded proteins are likely to participate in response to the two isolated fragments; RhCG (Rosa hybrida CG) and RhAA (Rosa hybrida AA) are more similar to putative protein associated to the senescence of Pisum sativum and Picea abies, respectively. There was no information for two fragments in the database. Reverse Northern blot demonstrated the credibility of the result of cDNA-AFLP. Study of expression pattern of the fragments showed an increase of expression level in almost all of them.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

36

Numer

01

Opis fizyczny

p.199-206,fig.,ref.

Twórcy

  • Department of Horticultural Science, Faculty of Agriculture, University of Maragheh, Maragheh, Iran
autor
  • Department of Horticultural Science, Faculty of Agriculture Science and Engineering, College of Agriculture and Natural Resources, University of Tehran, Kataj, Iran
autor
  • National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran-Kataj Highway, Tehran, Iran
autor
  • Department of Horticultural Science, Faculty of Agriculture Science and Engineering, College of Agriculture and Natural Resources, University of Tehran, Kataj, Iran
autor
  • National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran-Kataj Highway, Tehran, Iran

Bibliografia

  • Adam Z, Borochov A, Mayak S, Halevy AH (2006) 0 Correlative changes in sucrose uptake, ATPase activity and membrane fluidity in carnation petals during senescence. Physiol Planta 58:257–262.
  • Altschul SF, Madden TL, Schaffer AA, Zhang J, Zhang Z, Miller Bachem CWB, Van der Hoeven RS, De Bruijn SM, Vreugdenhil D, Zabeau M, Visser RGF (1996) Visualization of differential gene expression using a novel method of RNA fingerprinting based on AFLP: analysis of gene expression during potato tuber development. Plant J 9:745–753.
  • Bachem CW, van der Hoeven RS, de Bruijn SM, Vreugdenhil D, Zabeau M, Visser RG (1996) Visualization of differential gene expression using a novel method of RNA fingerprinting based on AFLP: analysis of gene expression during potato tuber development. Plant J 9:745–753.
  • Breyne P, Dreesen R, Cannoot B, Rombaut D, Vandepoele K, Rombauts S, Vanderhaeghen R, Inzé D, Zabeau M (2003) Quantitative cDNA-AFLP analysis for genome-wide expression studies. Mol Genet Genomics 2:173–179.
  • Buchanan-Wollaston V (1997) The molecular biology of leaf senescence. J Exp Bot 48:181–199.
  • Buchanan-Wollaston V, Morris K (2000) Senescence and cell death in Brassica napus an Arabidopsis. In: Bryant JA et al (eds) Programmed cell death in animals and plants. BIOS Scientific Publishers, Oxford, pp 163–174.
  • Bucher M, Brunner S, Zimmermann P, Zardi GI, Amrhein N, Willmitzer L, Riesmeier JW (2002) The Expression of an extensin-like protein correlates with cellular tip growth in tomato. Plant Physiol 128:911–923.
  • Chakrabarty D, Kumar Verma A, Kumar Datta S (2009) Oxidative stress and antioxidant activity as the basis of senescence in Hemerocallis (day lily) flowers. J Hort Forestry 7:113–119.
  • Dai N, Schaffer A, Petreikov M, Shahak Y, Giller Y, Ratner K, Levine A, Granot D (1999) Overexpression of Arabidopsis hexokinase in tomato plants inhibits growth, reduces photosynthesis, and induces rapid senescence. Plant Cell 11:1253–1266.
  • Ditt RF, Nester EW, Comai L (2001) Plant gene expression response to Agrobacterium tumefaciens. PNAS 98:10954–10959.
  • Eklund Magnus D, Edqvist J (2003) Localization of nonspecific lipid transfer proteins correlate with programmed cell death responses during endosperm degradation in Euphorbia lagascae seedlings. Plant Physiol 132:1249–1259.
  • Ezhilmathi K, Singh VP, Arora A, Sairam RK (2007) Effect of 5-sulfosalicylic acid on antioxidant activity in relation to vase life of Gladiolus cut flowers. Plant Growth Regul 2:99–108.
  • Fusco N, Micheletto L, Dal Corso G, Borgato L, Furini A (2005) Identification of cadmium-regulated genes by cDNA-AFLP in the heavy metal accumulator Brassica juncea L. J Exp Bot 56:3017–3027.
  • Gan S, Amasino RM (1997) Making sense of senescence-molecular genetic regulation and manipulation of leaf senescence. Plant Physiol 113:313–319.
  • Haddad R, Morris K, Buchanan-Wollaston V (2004) Expression analysis of genes related to oxidative protection during genesrelated to oxidative senescence in Brassica napus. J Agric Sci Technol 6:63–72.
  • Hajouj T, Michelis R, Gepstein S (2000) Cloning and characterization of a receptor-like protein kinase gene associated with senescence. Plant Physiol 124:1305–1314.
  • Hmida-Sayari A, Costa A, Leone A, Jaoua S, Gargouri-Bouzid R (2005) Identification of salt stress-induced transcripts in potato leaves by cDNA-AFLP. Mol Biotech 1:31–40.
  • Huffaker RC (1990) Proteolytic activity during the senescence of plants. New Phytol 116:193–231.
  • Hunter DA, Reid MS (2001) Senescence-associated gene expression in narcissus ‘Dutch master’. Acta Hort 553:341–343.
  • Jones ML, Larsen PB, Woodson WR (1995) Ethylene-regulated expression of a carnation cysteine proteinase during flower petal senescence. Plant Mol Biol 28:505–512.
  • Kader JC (1996) Lipid-transfer proteins in plants. Annu Rev Plant Physiol Plant Mol Biol 47:627–654.
  • Kornmann B, Preitner N, Rifat D, Fleury-Olela F, Schibler U (2001) Analysis of circadian liver gene expression by ADDER, a highly sensitive method for the display of differentially expressed mRNAs. Nucleic Acids Res 29:e51.
  • Nabigol A, Naderi R, Mostofi Y, Khalighi A, Bujar M (2009) Soluble carbohydrates content and ethylene production in cut rose cultivars. Hort Environ Biotechnol 50:122–126.
  • Nell TA, Leonard RT (2004) Special research report: post production, identifying long-lasting cut rose varieties. www.Endowment.org.
  • Polegri L, Calderini O, Arcioni S, Pupilli F (2010) Specific expression of apomixis-linked alleles revealed by comparative transcriptomic analysis of sexual and apomictic Paspalum simplex Morong flowers. J Exp Bot 6:1869–1883.
  • Qin L, Overmars H, Helder J, Popeijus H, van der Rouppe, Voort J, Groenink W, van Koert P, Schots A, Bakker J, Smant G (2000) An efficient cDNA-AFLP-based strategy for the identification of putative pathogenicity factors from the potato cyst nematode Globodera rostochiensis. Mol Plant-Microbe Interact 13:830–836.
  • Shimizu T, Inoue T, Shiraishi H (2002) Cloning and characterization of novel extensin-like cDNAs that are expressed during late somatic cell phase in the green alga Volvox carteri. Gene 284:179–187.
  • Thompson JE, Froese CD, Madey E, Smith MD, Hong Y (1998) Lipid metabolism during petal senescence. Prog Lipid Res 37:119–141.
  • Van Doorn WG, Stead AD (1997) Abscission of flowers and floral parts. J Exp Bot 48:821–837.
  • Van Doorn WG, Woltering EJ (2008) Physiology and molecular biology of petal senescence. J Exp Bot 59:453–480. doi:10.1093/jxb/erm356.
  • Wang D, Fan J, Ranu RS (2004) Cloning and expression of 1-aminocyclopropane-1-carboxylate synthase cDNA from rosa (Rosa X Hybrida). Plant Cell Rep 22:422–429.
  • Wu C, Zhou S, Zhang Q, Zhao W, Peng Y (2006) Molecular cloning and differential expression of an Y-aminobutyrate transaminase gene, OsGABA-T, in rice (Oryza sativa) with blast fungus. J Plant Res 119:663–669.
  • Xu Y, Hiroyuki I, Daniel R, Hanson MR (2006) Upregulation of a tonoplast-localized cytochrome P450 during petal senescence in petunia inflata. BMC Plant Biol 6:8. doi:10.1186/1471-2229-6-8.
  • Yoshida S, Ito M, Nishida I, Watanabe A (2001) Isolation and RNA gel blot analysis of genes that could serve as potential molecular markers for leaf senescence in Arabidopsis thaliana. Plant Cell Physiol 42:170–178.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

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