Immunopresence and functional activity of prostaglandin - endoperoxide synthases and nitric oxide synthases in bovine corpora lutea during diestrus

• Autorzy: Zerani M. , Catone G. , Betti G. , Parillo F.
• Języki publikacji: EN

Abstrakty

EN

The aim of this study was to evaluate the occurrence and the activity of prostaglandin-endoperoxide synthase 1 (PTGS1), PTGS2, and endothelial, neuronal, and inducible nitric oxide synthase (e-, n-, and iNOS) in early, mid, late, and regressive corpora lutea (CL) of bovines during diestrus. PTGS1 immunoreactivity was localised mainly in the cytoplasm of small luteal cells, whereas PTGS2 was detected in the cytoplasm of large luteal cells during early, mid, and late stages. The immunoexpression of all NOS isoforms was observed in the nuclei of luteal cells in the CL stages examined. PTGS1 enzyme activity was higher in late CL and lower in regressive ones; PTGS2 increased from early to late CL and lowered in regressive ones. Constitutive NOS enzymatic activity (eNOS plus nNOS) was higher in late CL and lower in regressive ones; iNOS was lower in regressive CL. These results support the idea that PTGSs and NOSs regulate the bovine CL life span mainly during the transition from the luteotrophic to the luteolytic phase. (Folia Morphol 2013; 72, 1: 36–40)

• Wydawca: -
• Czasopismo: Folia Morphologica
• Rocznik: 2013
• Tom: 72
• Numer: 1
• Opis fizyczny: p.36-40,fig.,ref.

Twórcy

autor

Zerani M.

• Scuola di Scienze Mediche Veterinarie, Universita di Camerino, via Circonvallazione 93, I-62024 Matelica, Italy

autor

Catone G.

• Scuola di Scienze Mediche Veterinarie, Universita di Camerino, via Circonvallazione 93, I-62024 Matelica, Italy

autor

Betti G.

• Scuola di Scienze Mediche Veterinarie, Universita di Camerino, via Circonvallazione 93, I-62024 Matelica, Italy

autor

Parillo F.

• Scuola di Scienze Mediche Veterinarie, Universita di Camerino, via Circonvallazione 93, I-62024 Matelica, Italy

Bibliografia

• 1. Arosh JA, Parent J, Chapdelaine P, Sirois J, Fortier MA (2002) Expression of cyclooxygenases 1 and 2 and prostaglandin E synthase in bovine endometrial tissue during the estrous cycle. Biol Reprod, 67: 161–169.
• 2. Arosh JA, Banu SK, Chapdelaine P, Madore E, Sirois J, Fortier MA (2004) Prostaglandin biosynthesis, transport and signaling in corpus luteum: a basis for autoregulation of luteal function. Endocrinology, 145: 2551–2560.
• 3. Catone G, Zerani M, Russo M, Mancuso R, Parillo F (2009) Presence and enzymatic activity of COX1, COX2 and NOS in bovine corpora lutea during luteal phase. Reprod Dom Anim, 44 (suppl. 3): 99.
• 4. Catone G, Zerani M, Castellucci B, Ciccarelli M, Currenti M, Gobbetti A, Parillo F (2011). Gonadotropin-releasing hormone (GnRH) receptor expression and in vitro GnRH effects in early-, mid- and late-corpora lutea of mediterranean buffalo (Bubalus bubalis). Reprod Dom Anim, 46 (suppl. 3): 93–94.
• 5. Davis JS, Rueda BR (2002) The corpus luteum: an ovarian structure with maternal instincts and suicidal tendencies. Front Biosci, 7: 1949–1978.
• 6. Guelfi G, Zerani M, Brecchia G, Parillo F, Dall’Aglio C, Maranesi M, Boiti C (2011) Direct actions of ACTH on ovarian function of pseudopregnant rabbits. Mol Cell Endocrinol, 339: 63–71.
• 7. Herzog K, Brockhan-Lüdemann M, Kaske M, Beindorff N, Paul V, Niemann H, Bollwein H (2010) Luteal blood flow is a more appropriate indicator for luteal function during the bovine estrous cycle than luteal size. Theriogenology, 73: 691–697.
• 8. McCann SM, Mastronardi C, De Laurentiis A, Rettori V (2005) The nitric oxide theory of aging revisited. Ann NY Acad Sci, 1057: 64–84.
• 9. Parillo F, Catone G, Boiti C, Zerani M (2011a) Immunopresence and enzymatic activity of nitric oxide synthases, cyclooxygenases and PGE2-9-ketoreductase and in vitro production of PGF2alpha, PGE2 and testosterone in the testis of adult and prepubertal alpaca (Lama pacos). Gen Comp Endocrinol, 171: 381–388.
• 10. Parillo F, Catone G, Castellucci B, Vullo C, Betti G, Mari S, Zerani M (2011b) Immunopresence and enzymatic activity of nitric oxide syntases, cyclooxygenases, and PGE2-9-ketoreductase and in vitro production of progesterone, PGF2alpha, and PGE2 in mediterranean buffalo (Bubalus bubalis) corpora lutea during diestrus. Reprod Dom Anim, 46 (suppl. 3): 138.
• 11. Parillo F, Catone G, Maranesi M, Gobbetti A, Gasparrini B, Russo M, Boiti C, Zerani M (2012) Immunolocalization, gene expression, and enzymatic activity of cyclooxygenases, prostaglandin E2-9-Ketoreductase, and nitric oxide synthases in Mediterranean buffalo (Bubalus bubalis) corpora lutea during diestrus. Micr Res Techn, doi: 10.1002/jemt.22116.
• 12. Rosiansky-Sultan M, Klipper E, Spanel-Borowski K, Meidan R (2006) Inverse relationship between nitric oxide synthases and endothelin-1 synthesis in bovine corpus luteum: interactions at the level of luteal endothelial cell. Endocrinology, 147: 5228–5235.
• 13. Skarzynski DJ, Jaroszewski JJ, Bah MM, Deptula KM, Barszczewska B, Gawronska B, Hansel W (2003) Administration of a nitric oxide synthase inhibitor counteracts prostaglandin F2-induced luteolysis in cattle. Biol Reprod, 68: 1674–1681.
• 14. Watanabe K (2002) Prostaglandin F synthase. Prostaglandins Other Lipid Mediat, 68–69: 401–407.
• 15. Wiltbank MC, Ottobre JS (2003) Regulation of intraluteal production of prostaglandins. Reprod Biol Endocrinol, 1: 91.
• 16. Zerani M, Dall’Aglio, C, Maranesi M, Gobbetti A, Brecchia G, Mercati F, Boiti C (2007) Intraluteal regulation of prostaglandin F2alpha-induced prostaglandin biosynthesis in pseudopregnant rabbits. Reproduction, 133: 1005–1016.
• 17. Zerani M, Parillo F, Brecchia G, Guelfi G, Dall’Aglio C, Lilli L, Maranesi M, Gobbetti A, Boiti C (2010) Expression of type I GNRH receptor and in vivo and in vitro GNRH-I effects in corpora lutea of pseudopregnant rabbits. J Endocrinol, 207: 289–300.
• 18. Zerani M, Catone G, Quassinti L, Maccari E, Bramucci M, Gobbetti A, Maranesi M, Boiti C, Parillo F (2011) In vitro effects of GnRH on Leydig cells of adult alpaca (Lama pacos) testis: GnRH receptor immunolocalization, testosterone and prostaglandin synthesis, and cyclooxygenase activities. Dom Anim Endocrinol, 40: 51–59.
• 19. Zerani M, Catone G, Maranesi M, Gobbetti A, Boiti C, Parillo F (2012) Gonadotropin-releasing hormone 1 directly affects corpora lutea lifespan in mediterranean buffalo (Bubalus bubalis) during diestrus: presence and in vitro effects on enzymatic and hormonal activities. Biol Reprod, 87: 1–8.
• 20. Zerani M, Maranesi M, Brecchia G, Gobbetti A, Boiti C, Parillo F (2013) Evidence for a luteotropic role of peroxisome proliferator-activated receptor gamma: expression and in vitro effects on enzymatic and hormonal activities in corpora lutea of pseudopregnant rabbits. Biol Reprod, DOI: 10.1095/biolreprod.112.10.7383.