Genetic structure among four populations of paddlefish, Polyodon spathula (Actinopterygii: Acipenseriformes: Polyodontidae), based on disomic microsatellite markers

• Autorzy: Zheng X. , Schneider K. , Lowe J. D. , Gomelsky B. , Mims S. D. , Bu S.
• Języki publikacji: EN

Abstrakty

EN

Background. The paddlefish, Polyodon spathula (Walbaum, 1792), is an important species for commercial and recreational fisheries throughout the central United States. Populations have declined in many areas due to river modification, loss of spawning habitat, pollution, and over-exploitation. Assessing genetic diversity of a species is an important consideration for developing conservation plans. The goal of this research was to perform a broad range survey of paddlefish diversity by evaluating populations from geographically distant major rivers of the United States of America. Materials and Methods. Paddlefish samples were collected from four sites including the Alabama River, Red River, Yellowstone/Missouri River, and Ohio River. Eight microsatellite loci (PspD102, PspD111, PspB105, PspD9, PspD8, PspC6, PspH26, and PspC10) that displayed disomic inheritance patterns were used for the amplification of alleles. Results. Average allelic richness of four sites ranged from 7.50 ± 1.36 to 5.46 ± 0.91. Average expected heterozygosity ranged from 0.717 ± 0.085 to 0.591 ± 0.093, the average observed heterozygosity assumed the values from 0.711 ± 0.115 to 0.585 ± 0.087. A moderate level of between population diversity was observed with an overall Fst value of 0.0702. Hardy–Weinberg equilibrium revealed that seven loci in the four populations were in equilibrium. The four populations were clustered to two categories by cluster analysis (UPGMA) based on Fst and δμ2 distance. Conclusion. Four studied paddlefish populations exhibited relatively low levels of genetic diversity and close relative relations, but still had some differentiation among the populations. The genetic distance and Fst revealed that the Ohio River, Red River and Yellowstone/Missouri River populations belong to the same branch, while the Alabama River population from another branch.

• Wydawca: -
• Czasopismo: Acta Ichthyologica et Piscatoria
• Rocznik: 2014
• Tom: 44
• Numer: 3
• Opis fizyczny: p.213-219,fig.,ref.

Twórcy

autor

Zheng X.

• Forestry College and College of Life Sciences, Northwest A&F University, 8 Xinong Road, Yangling, Shaanxi, 712100, China
• Aquaculture Research Center, Kentucky State University, 103 Athletic Road, Frankfort, KY 40601, USA

autor

Schneider K.

• Aquaculture Research Center, Kentucky State University, 103 Athletic Road, Frankfort, KY 40601, USA

autor

Lowe J. D.

• College of Agriculture Food Science and Sustainable Systems, Kentucky State University, 103 Athletic Road, Frankfort, KY 40601, USA

autor

Gomelsky B.

• Aquaculture Research Center, Kentucky State University, 103 Athletic Road, Frankfort, KY 40601, USA

autor

Mims S. D.

• Aquaculture Research Center, Kentucky State University, 103 Athletic Road, Frankfort, KY 40601, USA

autor

Bu S.

• Forestry College and College of Life Sciences, Northwest A&F University, 8 Xinong Road, Yangling, Shaanxi, 712100, China
• Aquaculture Research Center, Kentucky State University, 103 Athletic Road, Frankfort, KY 40601, USA

Bibliografia

• Barton N.H., Slatkin M. 1986. A quasi-equilibrium theory of the distribution of rare alleles in a subdivided population. Heredity 56 (3): 409–415. DOI: 10.1038/hdy.1986.63
• Braaten P.J., Fuller D.B., Lott R.D. 2009. Spawning migrations and reproductive dynamics of paddlefish in the upper Missouri River basin, Montana and North Dakota. Pp. 103–122. In: Paukert C.P., Scholten G.D. (eds.) Paddlefish management, propagation, and conservation in the 21st Century, American Fisheries Society Symposium 66, Bethesda, MD, USA.
• Carlson D.M., Bonislawsky P.S. 1981. The paddlefish (Polyodon spathula) fisheries of the midwestern United States. Fisheries 6 (2): 17–27. DOI: 10.1577/1548-8446(1981)006<0017:TPPSFO>2.0.CO;2
• Carlson D.M., Ketler M.K., Fisher S.E., Whitt G.S. 1982. Low genetic variability in paddlefish populations. Copeia 1982 (3): 721–725. DOI: 10.2307/1444682
• Epifanio J.M., Koppelman J.B., Nedbal M.A., Philipp D.P. 1996. Geographic variation of paddlefish allozymes and mitochondrial DNA. Transactions of the American Fisheries Society 125 (4): 546–561. DOI: 10.1577/1548-8659(1996)125<0546:GVOPAA>2.3.CO;2
• Goldstein D.B., Pollock D.D. 1997. Launching microsatellites: A review of mutation processes and method for phylogenetic inference. Journal of Heredity 88 (5): 335–342.
• Graham K. 1997. Contemporary status of the North American paddlefish, Polyodon spathula. Environmental Biology of Fishes 48 (1–4): 279–289. DOI: 10.1023/A:1007397021079
• Guo S.W., Thompson E.A. 1992. Performing exact test of Hardy–Weinberg proportion for multiple alleles. Biometrics 48 (2): 361–372.
• Hardy O.J., Vekemans X. 2002. SPAGeDi: a versatile komputer program to analyze spatial genetic structure at the individual or population levels. Molecular Ecology Notes 2 (4): 618–620. DOI: 10.1046/j.1471-8286.2002.00305.x
• Heist E.J., Mustapha A. 2008. Rangewide genetic structure In paddlefish inferred from DNA microsatellite loci.Transactions of the American Fisheries Society 137 (3): 909–915. DOI: 10.1577/T07-078.1
• Jennings C.A., Zigler S.J. 2000. Ecology and biology of paddlefish in North America: Historical perspectives, management approaches, and research priorities. Reviews in Fish Biology and Fisheries 10 (2): 167–181.DOI: 10.1023/A:1016633604301
• Johnson W.E., Eizirick E.,Roelke-Parker M.,O’Brien S.J. 2001. Applications of Genetic Concepts andMolecularMethods to Carnivore Conservation. Pp. 335–358. In: Gittleman J.L.,Funk S.M., MacDonald D., Wayne R.K. (eds.) Carnivore Conservation. University Press, The Zoological Society of London, Cambridge, UK.
• Jones K.C., Levine K.F., Banks J.D. 2002. Characterization of 11 polymorphic tetranucleotide microsatellites for forensic applications in California elk (Cervus elaphus canadensis). Molecular Ecology Notes 2 (4): 425–427. DOI:10.1046/j.1471-8286.2002.00264.x
• Kalinowski S.T. 2005. HP-RARE 1.0: a computer program for performing rarefaction on measures of allelic diversity. Molecular Ecology Notes 5 (1): 187–189. DOI: 10.1111/j.1471-8286.2004.00845.x
• Lein G.M., DeVries D.R. 1998. Paddlefish in the Alabama River drainage: Population characteristics and the adult spawning migration. Transactions of the American Fisheries Society 127 (3): 441–454. DOI: 10.1577/1548-8659(1998)127<0441:PITARD>2.0.CO;2
• Nei M. 1978. Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics 89 (3): 583–590.
• Pasch R.W., Alexander C.M. 1986. Effects of commercial fishing on paddlefish populations. Pp. 46–53. In: Dillard J.G., Graham L.K., Russell T.R. (eds.) The paddlefish: Status, management and propagation. American Fisheries Society, North Central Division, Bethesda, MD, USA. Special Publication 7.
• Rice W.R. 1989. Analyzing tables of statistical tests. Evolution 43 (1): 223–225. DOI: 10.2307/2409177
• Rousset F. 2008. GENEPOP’007: a complete re-implementation of the GENEPOP software for Windows and Linux. Molecular Ecology Resources 8 (1): 103–106. DOI: 10.1111/j.1471-8286.2007.01931.x
• Scarnecchia D.L., Stewart P.A., Power G.J. 1996. Age structure of the Yellowstone-Sakakawea paddlefish stock, 1963–1993, in relation to reservoir history. Transactions of the American Fisheries Society 125 (2): 291–299. DOI: 10.1577/1548-8659(1996)125<0291:ASOTYS>2.3.CO;2
• Stancill W., Jordan G.R., Paukert C.P. 2002. Seasonal migration patterns and site fidelity of adult paddlefish in Lake Francis Case, Missouri River, North Dakota. North American Journal of Fisheries Management 22 (3):815–824. DOI: 10.1577/1548-8675(2002)022<0815: SMPASF>2.0.CO;2
• Szalanski A.L., Bischof R., Mesti G. 2000. Population genetic structure of Nebraska paddlefish based on mitochondria DNA variation. Transactions of the American Fisheries Society 129 (4): 1060–1065. DOI: 10.1577/1548-8659(2000)129<1060:PGSONP>2.3.CO;2
• Weir B.S., Cockerham C.C. 1984. Estimating F-statistics for the analysis of population structure. Evolution 38 (6): 1358–1370. DOI: 10.2307/2408641