Taxane production induced by methyl jasmonate in free and immobilized cell cultures of Mexican yew (Taxus globosa Schltdl)

• Autorzy: Osuna L. , Tapia N. , Cusido R. , Palazon J. , Bonfill M. , Zamilpa A. , Lopez-Upton J. , Cruz-Sosa F.
• Języki publikacji: EN

Abstrakty

EN

Taxus globosa Schltdl, the Mexican yew, represents a new source of taxanes, including taxol, baccatin III, 10-deacetylbaccatin III, 10-deacetyltaxol and cephalomannine. Due to the anticancer activity and other biological activities of these compounds, and their scarcity in nature, we initiated in vitro cultures of this species with the aim of developing a biotechnological process for obtaining taxol and related taxanes. In the current work, in a batch-type twophase culture of T. globosa, we evaluated the effect of cell immobilization and methyl jasmonate (MeJ) elicitation in two culture media containing different plant growth regulator combinations: 2,4-dichlorophenoxyacetic + benzylaminopurine (Treatment 1: T1) and picloram + kinetin (Treatment 2: T2). The productivity and excretion rate into the culture medium of baccatin III (12.79 lg L-1 d-1) (84 %), 10-deacetylbaccatin III (15 lg L-1 d-1) (0%), 10-deacetyltaxol (3.18 lg L-1 d-1) (63 %), and cephalomannine (49.27 lg L-1 d-1) (9 %) were increased by the effect of T1 in the free cell cultures elicited with MeJ. Cell immobilization in alginate beads did not improve the biotechnological production of these four taxanes. In contrast, the highest productivity of taxol (53 lg L-1 d-1) was achieved in MeJ-elicited free cells under T2 and cell immobilization in these conditions increased productivity by more than twofold (130.35 lg L-1 d-1).

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2015
• Tom: 37
• Numer: 10
• Opis fizyczny: Article: 199 [8 p.], fig.,ref.

Twórcy

autor

Osuna L.

• Laboratorio de Biotecnologia, Centro de Investigacion Biomedica del Sur, Instituto Mexicano del Seguro Social, Argentina 1, Col. Centro, 62790, Xochitepec, Morelos, Mexico

autor

Tapia N.

• Laboratorio de Biotecnologia, Centro de Investigacion Biomedica del Sur, Instituto Mexicano del Seguro Social, Argentina 1, Col. Centro, 62790, Xochitepec, Morelos, Mexico
• Departamento de Biotecnologia, Division de Ciencias Biologicas, Universidad Autonoma Metropolitana, Mexico City, Mexico
• Laboratorio de Fisiología Vegetal, Facultad de Farmacia, Universidad de Barcelona, Barcelona, Spain

autor

Cusido R.

• Laboratorio de Fisiologia Vegetal, Facultad de Farmacia, Universidad de Barcelona, Barcelona, Spain

autor

Palazon J.

• Laboratorio de Fisiologia Vegetal, Facultad de Farmacia, Universidad de Barcelona, Barcelona, Spain

autor

Bonfill M.

• Laboratorio de Fisiologia Vegetal, Facultad de Farmacia, Universidad de Barcelona, Barcelona, Spain

autor

Zamilpa A.

• Laboratorio de Biotecnologia, Centro de Investigacion Biomedica del Sur, Instituto Mexicano del Seguro Social, Argentina 1, Col. Centro, 62790, Xochitepec, Morelos, Mexico

autor

Lopez-Upton J.

• Colegio de Postgraduados, Texcoco, Edo. de Mexico, Mexico

autor

Cruz-Sosa F.

• Departamento de Biotecnología, Division de Ciencias Biologicas, Universidad Autonoma Metropolitana, Mexico City, Mexico

Bibliografia

• Arias ZAM, Angarita VMJ, Aguirre CAM, Restrepo FJM, Montoya VC (2009) Estratégias para incrementar la producción de metabolitos secundarios en cultivos de células vegetales. Rev Fac Nal Agr Medellin 62:4881–4895
• Baebler S, Hren M, Camloh M, Ravnikar M, Bohanec B, Plaper I, Ucman R, Zel J (2005) Establishment of cell suspensions cultures of yew (Taxus 9 media Rehd.) and assessment of their genomic stability. In Vitro Cell Dev Biol Plant 41:338–343
• Barradas-Dermitz DM, Hayward-Jones PM, Mata-Rosas M, Palmeros-Sánchez B, Platas-Barradas OBJ, Velasquez-Toledo RF (2010) Taxus globosa S. cell lines: initiation, selection and characterization in terms of growth, and baccatin III and paclitaxel production. Biocell 34:1–6
• Barrales CHJ, Soto HRM, Ramos VAC, Trejo TLI, Martínez VM, Ramirez GME, San Miguel ChR, Luna PGR, López UJ (2011) Extracción y cuantificación de taxoides por HPLC en hojas in situ y en callos inducidos in vitro de Taxus globosa Schlecht. Span J Rural Dev 2:103–114
• Barrios H, Zhang YL, Sandoval C, Xiao ZA (2009) Increase of taxol production in Taxus globosa Shoot callus by Chlorocholine Chloride. Open Nat Prod J 2:33–37
• Bentebibel S, Moyano E, Palazon J, Cusido RM, Bonfill M, Eibl R, Pinol MT (2005) Effects of immobilization by entrapment in alginate and scale-up on taxol and baccatin III production in cell suspension cultures of Taxus baccata. Biotechnol Bioeng 89:647–655
• Bonfill M, Palazón J, Cusidó RM, Joly S, Morales C, Piñol MT (2003) Influence of elicitors on taxane production and 3-hydroxy-3-methylglutaryl coenzyme A reductase activity in Taxus 9 media cells. Plant Physiol Biochem 41:91–96
• Bonfill M, Exposito O, Moyano E, Cusidó RM, Palazón J, Piñol T (2006) Manipulation by of paclitaxel and baccatin III production in Taxus baccata suspension cultures. In Vitro Cell Dev Biol Plant 42:422–426
• Bonfill M, Bentebibel S, Moyano E, Palazón J, Cusidó RM, Eibl R, Piñol MT (2007) Paclitaxel and baccatin III production induced by methyl jasmonate in free and inmobilized cells of Taxus baccata. Biología Plantarum 51(4):647–652
• Brunakova K, Babincova Z, Takác M, Cellárová E (2004) Selection of callus cultures of Taxus baccata L. as a potential source of paclitaxel production. Eng Life Sci 4:465–469
• Cusido RM, Palazon J, Bonfill M, Navia-Osorio A, Morales C, Pinol MT (2002) Improved paclitaxel and baccatin III production in suspension cultures of Taxus media. Biotechnol Prog 18:418–423
• De Vries HJ, Egberg C (1979) High-pressure liquid chromatographic determination of carbohydrates in food products: evaluation of a method. J Assoc Off Anal Chem 62:1292–1296
• Dong HD, Zhong JJ (2001) Significant improvement of taxane production in suspension cultures of Taxus chinensis by combining elicitation with sucrose feed. Biochem Eng J 8:145–150
• Duncan DR, Widholm JM (1990) Measurements of viability suitable for plant tissue cultures. Methods Mol Biol 6:29–37
• Expósito O, Syklowska-Baranek K, Moyado E, Onrubia M, Bonfill M, Palazón J, Cusidó RM (2010) Metabolic responses of Taxus media transformed cell cultures to the addition of methyl jasmonate. Biotechnol Prog 26(4):1145–1153
• Fett-Neto AG, Zhang WY, DiCosmo F (1994) Kinetics of taxol production, growth, and nutrient uptake in cell suspensions of Taxus cuspidata. Biotechnol Bioeng 44(2):205–210
• Furmanova M, Syklowska-Baranek K (2000) Hairy root cultures of Taxus x media var. Hicksii Rehd. As a new source of paclitaxel and 10-deacetilbaccatin III. Biotechnol Lett 22:683–686
• Gamborg OL, Miller RA, Ojima K (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res 50:151–158
• Gillet F, Roisin C, Fliniaux MA, Jacquin-Dubreuil A, Barbotin JN, Nava-Saucedo JE (2000) Immobilization of Nicotiana tabacum plant cell suspensions within calcium alginate gel beads for the production of enhanced mounts of scopolin. Enzyme Microb Technol 26:229–234
• Guerrero B, Castillo J, Aguilar MI, Delgado G (2000) 5a, 7b, 9a,10b, 13a-pentaacetoxy-4(20), Taxadieno (7b-acetoxy-Taxusin) and Other Constituents from the Bark of the Mexican Yew, Taxus globosa (Taxaceae). Rev Soc Quím Méx 44:148–150
• Hezari M, Ketchum REB, Gibson DM, Croteau R (1997) Taxol production and taxadiene synthase activity in Taxus canadensis cell suspensión cultures. Arch Biochem Biophys 337:185–190
• Ketchum REB, Gibson DM, Croteau R, Shuler ML (1999) The kinetics of taxoid accumulation in cell suspension cultures of Taxus following elicitation with methyl jasmonate. Biotechnol Bioeng 62:97–105
• Malik S, Cusidó RM, Hossein MM, Moyano E, Palazón J, Bonfill M (2011) Production of the anticancer drug taxol in Taxus baccata suspensión cultures: a review. Process Biochem 46:23–34
• McCown BH, Lloyd G (1981) Woody Plant Medium (WPM) a mineral nutrient formulation for microculture for Woody plant species. Hortic Sci 16:543
• Nicolau KC, Yang Z, Liu JJ, Ueno H, Nantermet PG, Guy RK, Claiborne CF, Renaud J, Couladouros EA, Paulvannan K, Sorensen EJ (1994) Total synthesis of taxol. Nature 367:630–634
• Onrubia M, Moyano E, Bonfill M, Exposito O, Palazon J, Cusido RM (2011) An approach to the molecular mechanism of methyl jasmonate and vanadyl sulphate elicitation in Taxus baccata cell cultures: the role of txs and bapt gene expression. Biochem Eng J 53:104–111
• Osuna L, Moyano E, Mangas S, Bonfill M, Cusidó RM, Piñol MT, Zamilpa A, Tortoriello J, Palazón J (2008) Immobilization of Galphimia glauca Cav plant cell suspensions for the production of enhanced amounts of Galphimine-B. Planta Med 74:94–99
• Seki M, Ohzora Ch, Takeda M, Furusaki S (1997) Taxol (Paclitaxel) production using free and immobilized cells of Taxus cuspidata. Biotechnol Bioeng 53:214–219
• Soto-Hernández M, Sanjurjó M, González-Garza MT, Cruz-Vega D, Giral-González F (2000) El Tejo mexicano (Taxus globosa) Potencial de su aprovechamiento en taxol. Ciencia Ergo Sum 7(3):277–279
• Strobel GA, Stierle A, Hess WM (1973) Taxol formation in yew Taxus. Plant Sci 92:1–12
• Tapia N, Zamilpa A, Bonfill M, Ventura E, Cruz-Vega D, Del Villar A, Cruz-Sosa F, Osuna L (2013) Effect of the culture medium and biotic stimulation on taxane production in Taxus globosa Schltdl in vitro cultures. Acta Physiol Plant 35:3447–3455
• van Rozendaal ELM, Lelyveld GP, Van Beek TA (2000) Screening of the needles of different yew species and cultivars for paclitaxel and related taxoids. Phytochemistry 53:383–389
• Yukimune Y, Tabata H, Higashi Y, Hara Y (1996) Methyl jasmonateinduced overproduction of paclitaxel and baccatin III in Taxus cell suspension cultures. Nat Biotechnol 14:1129–1132
• Zavala-Chávez F (2001) Análisis demográfico preliminar de Taxus globosa Schlecht en el Parque Nacional El Chico, Hidalgo, México. I: Población de adultos y algunas características del hábitat. Ciencia Ergo Sum 8:169–174

Identyfikatory

DOI

10.1007/s11738-015-1947-z