Phenotyping shows improved physiological traits and seed yield of transgenic wheat plants expressing the alfalfa aldose reductase under permanent drought stress

• Autorzy: Feher-Juhasz E. , Majer P. , Sass L. , Lantos C. , Csiszar J. , Turoczy Z. , Mihaly R. , Mai A. , Horvath G. V. , Vass I. , Dudits D. , Pauk J.
• Języki publikacji: EN

Abstrakty

EN

Members of the aldo–keto reductase family including aldose reductases are involved in antioxidant defense by metabolizing a wide range of lipid peroxidation-derived cytotoxic compounds. Therefore, we produced transgenic wheat genotypes over-expressing the cDNA of alfalfa aldose reductase gene. These plants consequently exhibit 1.5–4.3 times higher detoxification activity for the aldehyde substrate. Permanent drought stress was generated in the greenhouse by growing wheat plants in soil with 20 % water capacity. The control and stressed plants were monitored by a semi automatic phenotyping platform providing computer-controlled watering, digital and thermal imaging. Calculation of biomass values was based on the correlation (R²= 0.7556) between fresh weight and green pixel-based shoot surface area. The green biomass production by plants of the three transgenic lines was 12–26–41 % higher than the non-transgenic plants’ grown under water limitation. Thermal imaging of stressed nontransgenic plants indicated an elevation in the leaf temperature. The thermal status of transformants was similar at both normal and suboptimal water regime. In drought, the transgenic plants used more water during the growing season. The described phenotyping platform provided a comprehensive data set demonstrating the improved physiological condition of the drought stressed transgenic wheat plants in the vegetative growth phase. In soil with reduced water capacity two transgenic genotypes showed higher seed weight per plant than the control non-transgenic one. Limitation of greenhouse-based phenotyping in analysis of yield potential is discussed.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2014
• Tom: 36
• Numer: 03
• Opis fizyczny: p.663-673,fig.,ref.

Twórcy

autor

Feher-Juhasz E.

• Department of Biotechnology, Cereal Research Non-Profit Ltd., Also kikoto sor 9, Szeged, 6726, Hungary

autor

Majer P.

• Department of Biotechnology, Cereal Research Non-Profit Ltd., Also kikoto sor 9, Szeged, 6726, Hungary

autor

Sass L.

• Institute of Plant Biology, Biological Research Center HAS, Temesvari krt 62, Szeged, 6726, Hungary

autor

Lantos C.

• Department of Biotechnology, Cereal Research Non-Profit Ltd., Also kikoto sor 9, Szeged, 6726, Hungary

autor

Csiszar J.

• Department of Plant Biology, University of Szeged, Kozep fasor 52, Szeged, 6726, Hungary

autor

Turoczy Z.

• Institute of Plant Biology, Biological Research Center HAS, Temesvari krt 62, Szeged, 6726, Hungary

autor

Mihaly R.

• Department of Biotechnology, Cereal Research Non-Profit Ltd., Also kikoto sor 9, Szeged, 6726, Hungary

autor

Mai A.

• Institute of Plant Biology, Biological Research Center HAS, Temesvari krt 62, Szeged, 6726, Hungary

autor

Horvath G. V.

• Department of Plant Biology, University of Szeged, Kozep fasor 52, Szeged, 6726, Hungary

autor

Vass I.

• Institute of Plant Biology, Biological Research Center HAS, Temesvari krt 62, Szeged, 6726, Hungary

autor

Dudits D.

• Institute of Plant Biology, Biological Research Center HAS, Temesvari krt 62, Szeged, 6726, Hungary

autor

Pauk J.

• Department of Biotechnology, Cereal Research Non-Profit Ltd., Also kikoto sor 9, Szeged, 6726, Hungary

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Identyfikatory

DOI

10.1007/s11738-013-1445-0