Development of cost-effective tetra-ARMS PCR for detection of FecB genotype in sheep

• Autorzy: Guan F. , Shi G. , Wan P. , Dai R. , Tang H. , Wang H. , Luo Y.
• Języki publikacji: EN

Abstrakty

EN

The FecB gene has been shown to be crucial in reproduction in many sheep breeds. It is a single nucleotide polymorphism (SNP) located in the bone morphogenetic protein receptor IB (BMPRIB)gene. The current methods for genotyping the FecB mutation are either slow and laborious or expensive. In this report, a single-step amplification approach suitable for FecB genotyping method is described. Multiplex PCR was performed with four primers on the basis of tetraprimer amplification refractory mutation system PCR (tetra-primer ARMS PCR), then three FecB genotypes can be detected after electrophoresis. Genotyping results of the proposed multiplex PCR occurred to be in complete accordance with forced PCR-RFLP of all samples. It is a rapid and simple method for detection of FecB in a larger number of samples, and is suitable for other SNPs detection with specific primers even in most “low-tech” laboratories.

• Wydawca: -
• Czasopismo: Animal Science Papers and Reports
• Rocznik: 2014
• Tom: 32
• Numer: 3
• Opis fizyczny: p.229-237,fig.,ref

Twórcy

autor

Guan F.

• College of Life Sciences, China Jiliang University, Zhejiang Provincial Key Laboratory of Biometrology and Inspection and Quarantine, Institute of Plant Environmental Biology and Measurement Technology, Hangzhou 310018, China

autor

Shi G.

• The Key Sheep Breeding and Reproduction Biotechnology Laboratory of Xinjiang Production and Construction Group, Research Institute of Animal Husbandry and Veterinary, Xinjiang Agricultural Reclamation Academy, Shihezi 832000, China

autor

Wan P.

• The Key Sheep Breeding and Reproduction Biotechnology Laboratory of Xinjiang Production and Construction Group, Research Institute of Animal Husbandry and Veterinary, Xinjiang Agricultural Reclamation Academy, Shihezi 832000, China

autor

Dai R.

• The Key Sheep Breeding and Reproduction Biotechnology Laboratory of Xinjiang Production and Construction Group, Research Institute of Animal Husbandry and Veterinary, Xinjiang Agricultural Reclamation Academy, Shihezi 832000, China

autor

Tang H.

• The Key Sheep Breeding and Reproduction Biotechnology Laboratory of Xinjiang Production and Construction Group, Research Institute of Animal Husbandry and Veterinary, Xinjiang Agricultural Reclamation Academy, Shihezi 832000, China

autor

Wang H.

• College of Life Sciences, China Jiliang University, Zhejiang Provincial Key Laboratory of Biometrology and Inspection and Quarantine, Institute of Plant Environmental Biology and Measurement Technology, Hangzhou 310018, China

autor

Luo Y.

• College of Life Sciences, China Jiliang University, Zhejiang Provincial Key Laboratory of Biometrology and Inspection and Quarantine, Institute of Plant Environmental Biology and Measurement Technology, Hangzhou 310018, China

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