Influence of abiotic elicitation on production of dipyranocoumaris in suspension cultures of Calophyllum inophyllum L.

• Autorzy: Pawar K.D. , Thengane S. R.
• Języki publikacji: EN

Abstrakty

EN

Effects of elicitation with heavy metals such as copper, cadmium, chromium (abiotic elicitation) and supplementation of CaCl₂ on production of dipyranocoumarins (inophyllums) in suspension cultures of leaf and stem callus of Calophyllum inophyllum were studied. The optimum timing for elicitor introduction was found to be the 10th day after initiating the suspension cultures. Cadmium as abiotic elicitor in suspension cultures of stem callus was found best to elicit maximum production of inophyllums A, C, and calophyllolide while cadmium in suspension cultures of leaf callus was found best for eliciting maximum production of inophyllums B and P. Inophyllum D was the only dipyranocoumarin whose highest production was achieved when 1.0 mM chromium was used as abiotic elicitor in suspension cultures of stem callus. Out of the three abiotic elicitors used, none could result biomass growth. Only incorporation of CaCl₂ in suspension cultures resulted biomass growth. A maximum of 35.26-fold biomass growth was achieved when suspension cultures of stem callus were incorporated with 2.0 mMCaCl₂. CaCl₂ was noted to have no positive influence on production of most of the dipyranocoumarins under study.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2011
• Tom: 33
• Numer: 6
• Opis fizyczny: p.2149-2158,fig.,ref.

Twórcy

autor

Pawar K.D.

• Plant Tissue Culture Division, National Chemical Laboratory, Pune 411008, Maharashtra, India

autor

Thengane S. R.

• Plant Tissue Culture Division, National Chemical Laboratory, Pune 411008, Maharashtra, India

Bibliografia

• Baldi A, Singh D, Dixit VK (2008) Dual elicitation for improved production of withaferin A by cell SC of Withania somnifera. Appl Biochem Biotechnol 151:556–564
• Bhuiyan MNH, Adachi MK (2002) Variation in betalain content and factors affecting the biosynthesis in Portulaca sp. ‘Jewel’ cell culture. Plant Biotechnol 19:369–376
• Choudhury S, Panda SK (2005) Toxic effect oxidative stress and ultrastructural changes in moss Taxitheelium nepalense (Schwaegr.) broth under lead and chromium toxicity. Water Air Soil Pollut 167:73–90
• Faik SA, Locksley HD (1971) Xanthones in the heartwood of Calophyllum inophyllum: a geographical survey. Phytochemistry 10:603–606
• Fang Y, Smith MAL, Pepin MF (1999) Effect of exogenous methyl jasmonate in elicited anthocyanin—producing cell cultures of ohelo (Vaccinium pahalae). In vitro Cell Dev Biol Plant 35:106–113
• Furze JM, Rhodes MJC, Parr AJ, Robins RJ, Whithehead IM, Threlfall DR (1991) Abiotic factors elicit sesquiterpenoid phytoalexin production but not alkaloid production in transformed root cultures of Datura stramonium. Plant Cell Rep 10:111–114
• Govindachari TR, Viswanathan N, Pai BR, Rao R, Srinivasan M (1967) Triterpenes of Calophyllum inophyllum Linn. Tetrahedron 23(4):1901–1910
• Gutierrez MC, Parry A, Tena M, Jorrin J, Edwards R (1995) Abiotic elicitation of coumarin phytoalexins in sunflower. Phytochemistry 38:1185–1191
• Haraguchi H, Yano T, Tosa H, Iinuma M (2006) Jpn. Kokai Tokkoyo Koho JP 2006089661 A2 (6 April 2006), to (Eag KK, Japan)
• Jackson B, Locksley HD, Schienmann (1969) The isolation of 6-desoxyjacareubin, 2-(3, 3-dimethylallyl)-1, 3, 5, 6-tetrahydroxyxanthone and jacareubin form Calophyllum inophyllum. Phytochemistry 8:927–929
• Khan NUD, Perveen N, Singh MP, Singh R, Achari B, Partha PG, Dastidar Dutta PK (1996) Two isomeric benzodipyranone derivatives from Calophyllum inophyllum. Phytochemistry 42:1181–1183
• Kumar V, Ramchandran S, Sultanbawa MSU (1976) Xanthones and triterpenoids from timber of Calophyllum inophyllum. Phytochemistry 15:2016–2017
• Kuruyanagi M, Arakava T, Mikami Y, Yoshida K, Kawahar N, Hayashi T, Ishimaru H (1998) Phytoalexin from hairy root cultures of Hyoscymus albus treated with methyl jasmonate. J Nat Prod 61:1516–1519
• Laure F, Herbette G, Faure R, Bianchini JP, Raharivelomanana P, Fogliani B (2005) Structure of new secofriedelane and friedelane acids from Calophyllum inophyllum of French Polynesia. Magn Reson Chem 43:65–68
• Lee TK, Yamakawa T, Kodama T, Shimomura K (1998) Effects of chemicals on alkaloid production by transformed roots of Atropa belladona. Phytochemistry 49: 2343–2347
• Lloyd C, McCown B (1980) Commercially feasible micropropagation of mountain laurel, Kalmia latifolia by use of shoot tip culture. Int Plant Propag Soc Proc 30:421–427
• Munekazu M, Hideki T, Toshiyuki T, Shigetomo Y (1995) Two xanthones from roots of Calophyllum inophyllum. Phytochemistry 38:725–728
• Narula A, Kumar S, Bansal KC, Srivastava PS (2004) Biotechnological approaches towards improvement of medicinal plants. In: Srivasrava PS, Narula A, Srivastava S (eds) Plant biotechnology and molecular markers. Anamaya, New Delhi, pp 78–116
• Panda S, Choudhury S (2005) Changes in nitrate reductase activity and oxidative stress response in the moss Polytrichum commune subjected to chromium, copper and zinc toxicity. Braz J Plant Physiol 17(2):191–197
• Panda SK, Chaodhury I, Khan MH (2003) Heavy metal phytotoxicity induces lipid peroxidation and affect antioxidants in wheat leaves. Biol Plant 46:289–294
• Patil AD, Freyer AJ, Eggleston DS, Haltiwanger RC, Bean MF, Taylor PB, Caranfa MJ, Breen AL, Bartus HR, Johnson RK, Hertzberg RP, Westley JW (1993) The inophyllums, novel inhibitors of HIV-1 reverse transcriptase isolated from Malaysian tree, Calophyllum inophyllum Linn. J Med Chem 36:4132–4138
• Pawar KD, Thengane SR (2009) Influence of hormones and medium components on expression of dipyranocoumarins in cell suspension cultures of Calophyllum inophyllum L. Process Biochem 44:916–922
• Pawar KD, Joshi SP, Bhide SR, Thengane SR (2007) Pattren of anti-HIV dipyranocoumarins expression in callus cultures of Calophyllum inophyllum Linn. J Biotechnol 130:347–353
• Saba L, Pande D, Iqbal M, Srivastava PS (2000) Effect of ZnSO4 and CuSO4 on regeneration and lepidine content in Lepidium sativum. Biol Plant 43:253–256
• Sandalio LM, Dalurzo HC, Gomez M, Romero-Puertas MC, del Rio LA (2001) Cadmium induced changes in growth and oxidative metabolism of pea plants. J Exp Bot 52:2115–2126
• Sanita di Toppi L, Gabriella R (1999) Response to cadmium in higher plants. Environ Expt Bot 52:2115–2126
• Sanita di Toppi L, Prasad MNV, Ottonello S (2002) Metal chelating peptides and proteins in plants. In: Prasad MNV, Strzaka K (eds) Physiology and biochemistry of heavy metal detoxification and tolerance in plants. Kluwer Academic Publishers, Dordrecht, pp 59–93
• Tumova L, Blazkova R (2002) Effect on the formation of flavonoids in the culture of Ononis arvensis L. in vitro by the action of CrCl₃. Seska a Solvenska Farmacie 51(1):44–46
• Whitehead IM, Threlfall DR (1992) Production of phytoalexin by plant tissue cultures. J Biotechnol 26:63–81
• Yimdjo MC, Azebaze GA, Nkengfack AE, Meyer AM, Bodo B, Fomum ZT (2004) Antimicrobial and cytotoxic agents from Calophyllum inophyllum. Phytochemistry 65:2789–2795

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