Expression of integrin beta 2 (ITGB2) and zona pellucida glycoproteins (ZP3, ZP3alpha) in developmentally competent and incompetent porcine oocytes

• Autorzy: Antosik P. , Kempisty B. , Piotrowska H. , Bukowska D. , Ciesiolka S. , Jeseta M. , Piescikowska I. , Lange K. , Maryniak H. , Jaskowski J. M. , Brussow K. P. , Nowicki M. , Zabel M.
• Języki publikacji: EN

Abstrakty

EN

The fertilization potential of mammalian oocytes may be regulated at the molecular level by the expression of species-specific sperm-egg interaction molecules, whose activities and/or cellular distribution determine the recognition and fusion of gemetes. Although there exist studies on the expression of integrins (ITGs) and zona pellucida glycoproteins (ZPs) in developmentally fully competent oocytes, the mRNA levels encoding these proteins in immature and developmentally incompetent porcine oocytes have to be elucidated. Therefore, our aim was to determine the expression of ITGB2, ZP3, and ZP3α mRNAs in porcine oocytes before in vitro maturation (IVM), in oocytes stained with BCB test but colorless, and in BCB positive oocytes after IVM. Porcine cumulus-oocyte complexes (COCs) were collected from 32 crossbred Landrace gilts, and then separated into three groups: (i) oocytes analyzed immediately after collection (n = 50), (ii) oocytes stained with brilliant cresyl blue (BCB+) and remained colorless (BCB–) (n = 50). After collection and/or staining and cultivation, all oocytes were denuded and analyzed regarding ITGB2, ZP3, and ZP3α by QT-PCR. We found a higher expression of ITGB2, ZP3, and ZP3α in oocytes immediately after collection and in BCB+ oocytes compared to BCB– oocytes (P < 0.001, respectively). No differences in the ITGB2 and ZP3 mRNA levels were observed between oocytes after collection and BCB+ oocytes. In addition, BCB– oocytes revealed lower transcript expression of all the genes under study. It is presumed that the similar mRNA levels of ITGB2 and ZP3 in oocytes after collection and in BCB+/ IVM oocytes may be related to (i) a toxic effect of BCB staining and/or to (ii) the degradation of accumulated maternal templates in porcine oocytes. Additionally, lower ITGB2, ZP3, and ZP3α transcript levels point to the down-regulation of the mRNA synthesis of stored maternal transcripts in developmentally incompetent porcine oocytes.

• Wydawca: -
• Czasopismo: Medycyna Weterynaryjna
• Rocznik: 2014
• Tom: 70
• Numer: 07
• Opis fizyczny: p.417-421,fig.,ref.

Twórcy

autor

Antosik P.

• Institute of Veterinary Sciences, Poznan University of Life Science, 52 Wojska Polskiego St., 60-628 Poznan, Poland

autor

Kempisty B.

• Department of Histology and Embryology, Poznan University of Medical Science, 6 Swiecickiego St., 60-781 Poznan, Poland
• Department of Anatomy, Poznan University of Medical Science, 6 Swiecickiego St., 60-781 Poznan, Poland

autor

Piotrowska H.

• Department of Toxicology, Poznan University of Medical Sciences, 30 Dojazd St., 60-631 Poznan, Poland

autor

Bukowska D.

• Institute of Veterinary Sciences, Poznan University of Life Science, 52 Wojska Polskiego St., 60-628 Poznan, Poland

autor

Ciesiolka S.

• Department of Histology and Embryology, Poznan University of Medical Science, 6 Swiecickiego St., 60-781 Poznan, Poland

autor

Jeseta M.

• Department of Genetic and Reproduction, Reproductive Biotechnology Unit, Veterinary Research Institute, Brno, Czech Republic

autor

Piescikowska I.

• Department of Histology and Embryology, Poznan University of Medical Science, 6 Swiecickiego St., 60-781 Poznan, Poland

autor

Lange K.

• Department of Inorganic and Analytical Chemistry, Karol Marcinkowski University of Medical Sciences, Grunwaldzka 6, 60-780 Poznan, Poland

autor

Maryniak H.

• Institute of Zoology, Department of Animal Anatomy, Poznan University of Life Science, 71C Wojska Polskiego St., 60-625 Poznan, Poland

autor

Jaskowski J. M.

• Institute of Veterinary Sciences, Poznan University of Life Science, 52 Wojska Polskiego St., 60-628 Poznan, Poland

autor

Brussow K. P.

• Institute of Reproductive Biology, Leibniz Institute for Farm Animal Biology,Wilhelm-Stahl-Allee 2, 18196 Dummerstorf, Germany

autor

Nowicki M.

• Department of Histology and Embryology, Poznan University of Medical Science, 6 Swiecickiego St., 60-781 Poznan, Poland

autor

Zabel M.

• Department of Histology and Embryology, Wroclaw Medical University, 6a Chalubinskiego St., 50-368, Wroclaw, Poland

Bibliografia

• 1. Antosik P., Kempisty B., Bukowska D., Jackowska M., Wlodarczyk R., Budna J., Brüssow K. P., Lianeri M., Jagodzinski P. P., Jaskowski J. M.: Follicular size is associated with the levels of transcripts and proteins of selected molecules responsible for the fertilization ability of oocytes of puberal gilts. J. Reprod. Dev. 2009, 55, 588-593.
• 2. Bansal F., Gupta S. K.: Binding characteristics of sperm with recombinant human zona pellucida glycoprotein-3 coated beads. Indian J. Med. Res. 2009, 130, 37-43.
• 3. Blanco M. R., Demyda S., Moreno Millán M., Genero E.: Developmental competence of in vivo and in vitro matured oocytes: A review. Biotechnol. Mol. Biol. Rev. 2011, 6, 155-165.
• 4. Bromfield E. G., Nixon B.: The function of chaperone proteins in the assemblage of protein complexes involved in gamete adhesion and fusion processes. Reproduction 2013, 145, R31-42.
• 5. Depa-Martynow M., Kempisty B., Lianeri M., Jagodzinski P. P., Jedrzejczak P.: Association between fertilin beta, protamines 1 and 2 and spermatid-specific linker histone H1-like protein mRNA levels, fertilization ability of human spermatozoa, and quality of preimplantation embryos. Folia Histochem. Cytobiol. 2007, 45, 79-85.
• 6. Fabryova K., Simon M.: Function of the cell surface molecules (CD molecules) in the reproduction processes. Gen. Physiol. Biophys. 2009, 28, 1-7.
• 7. Fusi F., Bronson R. A.: Failure to detect integrins of the beta-2 subclass on the human sperm surface. Am. J. Reprod. Immunol. 1991, 25, 143-145.
• 8. Gadella B. M.: Dynamic regulation of sperm interactions with the zona pellucida prior to and after fertilisation. Reprod. Fertil. Dev. 2012, 25, 26-37.
• 9. Gupta S. K., Bhandari B., Shrestha A., Biswal B. K., Palaniappan C., Malhotra S. S., Gupta N.: Mammalian zona pellucida glycoproteins: structure and function during fertilization. Cell Tissue Res. 2012, 349, 665-678.
• 10. Gupta S. K., Chakravarty S., Suraj K., Bansal P., Ganguly A., Jain M. K., Bhandari B.: Structural and functional attributes of zona pellucida glycoproteins. Soc. Reprod. Fertil. Suppl. 2007, 63, 203-216.
• 11. Howes E., Pascall J. C., Engel W., Jones R.: Interactions between mouse ZP2 glycoprotein and proacrosin; a mechanism for secondary binding of sperm to the zona pellucida during fertilization. J. Cell. Sci. 2001, 114, 4127-4136.
• 12. Hynes R. O.: Integrins: bidirectional, allosteric signaling machines. Cell 2002, 110, 673-687.
• 13. Jackowska M., Kempisty B., Antosik P., Bukowska D., Budna J., Lianeri M., Rosinska E., Wozna M., Jagodzinski P. P., Jaskowski J. M.: The morphology of porcine oocytes is associated with zona pellucida glycoprotein transcript contents. Reprod. Biol. 2009, 9, 79-85.
• 14. Jansen S., Ekhlasi-Hundrieser M., Töpfer-Petersen E.: Sperm adhesion molecules: structure and function. Cells Tissues Organs 2001, 168, 82-92.
• 15. Jaslow C. R., Patterson K. S., Cholera S., Jennings L. K., Ke R. W., Kutteh W. H.: CD9 expression by human granulosa cells and platelets as a predictor of fertilization success during IVF. Obstet. Gynecol. Int. 2010, doi: 10.1155/2010/192461.
• 16. Jovine L., Qi H., Williams Z., Litscher E., Wassarman P. M.: The ZP domain is a conserved module for polymerization of extracellular proteins. Nat. Cell Biol. 2002, 4, 457-461.
• 17. Kempisty B., Antosik P., Bukowska D., Jackowska M., Lianeri M., Jaskowski J. M., Jagodzinski P. P.: Assessment of zona pellucida glycoprotein and integrin transcript contents in porcine oocytes. Reprod. Biol. 2009, 9, 71-78.
• 18. Kempisty B., Depa-Martynow M., Lianeri M., Jedrzejczak P., Darul-Wasowicz A., Jagodzinski P. P.: Evaluation of protamines 1 and 2 transcript contents in spermatozoa from asthenozoospermic men. Folia Histochem. Cytobiol. 2007, 45, 109-113.
• 19. Kempisty B., Jackowska M., Piotrowska H., Antosik P., Wozna M., Bukowska D., Brüssow K. P., Jaskowski J. M.: Zona pellucida glycoprotein 3 (pZP3) and integrin β2 (ITGB2) mRNA and protein expression in porcine oocytes after single and double exposure to brilliant cresyl blue test. Theriogenology 2011, 75, 1525-1535.
• 20. Kim M. K., Fibrianto Y. H., Oh H. J., Jang G., Kim H. J., Lee K. S., Kang S. K., Lee B. C., Hwang W. S.: Effects of estradiol-17beta and progesterone supplementation on in vitro nuclear maturation of canine oocytes. Theriogenology 2005, 63, 1342-1353.
• 21. Kölle S., Dubois C. S., Caillaud M., Lahuec C., Sinowatz F., Goudet G.: Equine zona protein synthesis and ZP structure during folliculogenesis, oocyte maturation, and embryogenesis. Mol. Reprod. Dev. 2007, 74, 851-859.
• 22. Larson J. E., Krisher R. L., Lamb G. C.: Effects of supplemental progesterone on the development, metabolism and blastocyst cell number of bovine embryos produced in vitro. Reprod. Fertil. Dev. 2011, 23, 311-318.
• 23. Lay K. M., Ashizawa K., Nakada T., Tatemoto H.: N-glycosylation of zona glycoproteins during meiotic maturation is involved in sperm-zona pellucida interactions of porcine oocytes. Theriogenology 2011a, 75, 1146-1152.
• 24. Lay K. M., Oshiro R., Arasaki C., Ashizawa K., Tatemoto H.: Role of acidification elicited by sialylation and sulfation of zona glycoproteins during oocyte maturation in porcine sperm-zona pellucida interactions. J. Reprod. Dev. 2011b, 57, 744-751.
• 25. Opiela J., Kątska-Książkiewicz L.: The utility of Brilliant Cresyl Blue (BCB) staining of mammalian oocytes used for in vitro embryo production (IVP). Reprod. Biol. 2013, 177-183.
• 26. Orvieto R., Ben-Rafael Z., Abir R., Bar-Hava I., Fisch B., Molad Y.: Controlled ovarian hyperstimulation: a state of neutrophil activation. Am. J. Reprod. Immunol. 1999, 42, 288-291.
• 27. Pawlak P., Pers-Kamczyc E., Renska N., Kubickova S., Lechniak D.: Disturbances of nuclear maturation in BCB positive oocytes collected from peri-pubertal gilts. Theriogenology 2011, 832-840.
• 28. Rath D., Töpfer-Petersen E., Michelmann H. W., Schwartz P., von Witzendorff D., Ebeling S., Ekhlasi-Hundrieser M., Piehler E., Petrunkina A., Romar R.: Structural, biochemical and functional aspects of sperm-oocyte interactions in pigs. Soc. Reprod. Fertil. Suppl. 2006, 62, 317-330.
• 29. Redgrove K. A., Anderson A. L., McLaughlin E. A., O’Bryan M. K., Aitken R. J., Nixon B.: Investigation of the mechanisms by which the molecular chaperone HSPA2 regulates the expression of sperm surface receptors involved in human sperm-oocyte recognition. Mol. Hum. Reprod. 2013, 19, 120-135.
• 30. Somfai T., Ozawa M., Noguchi J., Kaneko H., Karja N. W., Fahrudin M., Nakai M., Maedomari N., Dinnyés A., Nagai T., Kikuchi K.: In vitro development of polyspermic porcine oocytes: Relationship between early fragmentation and excessive number of penetrating spermatozoa. Anim. Reprod. Sci. 2008, 107, 131-147.
• 31. Thellin O., Zorzi W., Lakaye B., De Borman B., Coumans B., Hennen G., Grisar T., Igout A., Heinen E.: Housekeeping genes as internal standards: use and limits. J. Biotechnol. 1999, 75, 291-295.
• 32. Wang W. H., Abeydeera L. R., Prather R. S., Day B. N.: Morphologic comparison of ovulated and in vitro-matured porcine oocytes, with particular reference to polyspermy after in vitro fertilization. Mol. Reprod. Dev. 1998, 49, 308-316.
• 33. Willingham-Rocky L. A., Hinrichs K., Westhusin M. E., Kraemer D. C.: Effects of stage of oestrous cycle and progesterone supplementation during culture on maturation of canine oocytes in vitro. Reproduction 2003, 126, 501-508.
• 34. Wongsrikeao P., Otoi T., Yamasaki H., Agung B., Taniguchi M., Naoi H., Shimizu R., Nagai T.: Effects of single and double exposure to brilliant cresyl blue on the selection of porcine oocytes for in vitro production of embryos. Theriogenology 2006, 66, 366-372.
• 35. Yonezawa N., Kanai-Kitayama S., Kitayama T., Hamano A., Nakano M.: Porcine zona pellucida glycoprotein ZP4 is responsible for the sperm-binding activity of the ZP3/ZP4 complex. Zygote 2012, 20, 389-397.
• 36. Yu J., Deng M., Medvedev S., Yang J., Hecht N. B., Schultz R. M.: Transgenic RNAi-mediated reduction of MSY2 in mouse oocytes results in reduced fertility. Dev. Biol. 2004, 268, 195-206.

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