Inhibition of midgut protease of yellow stem borer (Scirpophaga incertulas) by cysteine protease-like inhibitor from mature jackfruit (Artocarpus heterophyllus) seed

• Autorzy: Shamim M. , Kahn N. A. , Singh K. N.
• Języki publikacji: EN

Abstrakty

EN

A novel protease inhibitor was isolated and purified from the mature seeds of jackfruit (Artocarpus heterophyllus) by precipitation with ammonium sulphate, followed by DEAE-cellulose and gel filtration (Sephadex G-100) chromatography. The isolated protease inhibitor strongly inhibited papain and midgut proteases of yellow stem borer (Scipophaga incertulas) larvae, as seen by in vitro assay. The purified protease inhibitor was active over a wide range of pH with the maximum activity between pH 4 and 10. This protein was also stable up to 80°C, but the retained activity was lost at 100°C, when heated for 30 min. The molecular mass of the purified cysteine-like protease inhibitor is to be 14.50 kDa as determined by SDS-PAGE. Significant reduction in larval weight and mortality was observed, when fresh rice culms with protease inhibitor was feeded to the yellow stem borer larvae. These results may provide important information to control the yellow stem borer in rice with respect to naturally occurring insecticidal proteins. The observed differences would potentially translate into reductions in population growth of yellow stem borer, indicating a potential value of using jackfruit protease inhibitor for protecting rice plants against damage by the yellow stem borer.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2011
• Tom: 33
• Numer: 6
• Opis fizyczny: p.2249-2257,fig.,ref.

Twórcy

autor

Shamim M.

• Department of Plant Molecular Biology and Genetic Engineering, Narendra Deva University of Agriculture and Technology, Faizabad, India

autor

Kahn N. A.

• Department of Plant Molecular Biology and Genetic Engineering, Narendra Deva University of Agriculture and Technology, Faizabad, India

autor

Singh K. N.

• Department of Plant Molecular Biology and Genetic Engineering, Narendra Deva University of Agriculture and Technology, Faizabad, India

Bibliografia

• Abe K, Arai S (1985) Purification of a cysteine proteinase inhibitor from rice, Oryza sativa L. japonica. Agric Biol Chem 49: 3349–3350
• Abe K, Emori Y, Kondo H, Suzuki K, Arai S (1987) Molecular cloning of a cysteine proteinase inhibitor of rice (Oryzacystatin). J Biol Chem 262:1693–1697
• Alfonso-Rubi J, Ortego F, Castanera P, Carbonero P, Diaz I (2003) Transgenic expression of trypsin inhibitor CMe from barley in Indica and Japonica rice, confers resistance to the rice weevil Sitophilus oryzae. Transgene Res 12:23–31
• Arai S, Matsumoto I, Emori Y, Abe K (2002) Plant seed cystatin and their target enzyme of endogenous and exogenous origin. J Agric Food Chem 50:6612–6617
• Azzouza H, Cherquia A, Campana EDM, Rahbe'b Y, Duportb G, Jouaninc L, Kaiserd L, Giordanengoa P (2005) Effects of plant protease inhibitors, oryzacystatin I and soybean Bowman–Birk inhibitor, on the aphid Macrosiphum euphorbiae (Homoptera: Aphididae) and its parasitoid Aphelinus abdominalis (Hymenoptera: Aphelinidae). J Insect Physiol 1:75–86
• Barrett AJ (1972) A new assay for cathepsin B1 and other thiol proteinases. Ann Biochem 47:280–293
• Bhat AV, Pattabiraman TN (1989) Protease inhibitors from jackfruit seed (Artocarpus integrifolia). J Biosci 14:351–365
• Bown DP, Wilkinson HS, Gatehouse JA (1997) Differentially regulated inhibitor sensitive and insensitive protease genes from the phytophagous insect pest, Helicoverpa armigera, are members of complex multigene families. Insect Biochem Mol Biol 27(7):625–638
• Brar DS, Singh R.J, Mahal MS, Singh B (1994) Effectiveness of seedling root tip treatment with some insecticides for the control of sten borer on Basmati rice. Pest Manage Eco Zool 2(20):119–112
• Broadway RM, Duffey SS (1986) Plant proteinase inhibitors: mechanism of action and effect on the growth and digestive physiology of larval Heliothis zea and Spodoptera exigua. J Insect Physiol 32:827–833
• Brzin J, Popovic T, Retonja A, Puizdar VM (1998) Related cystatin inhibitors from leaf and from seed of Phasiolus vulgaris (L.). Plant Sci 138:17–26
• Burgess EPJ, Malone LA, Christelle JT (1996) Effects of two proteinase inhibitors on the digestive enzymes and survival of honey bees (Apis mellifera). J Insect Physiol 42:823–828
• Datta K, Vasquez A, Tu J, Torrizo L, Alam MF, Oliva N, Abrigo E, Khush GS, Datta SK (1998) Constitutive and tissue-specific differential expression of CryIA(b) gene in transgenic rice plants conferring enhanced resistance to insect pests. Theor Appl Genet 97:20–30
• Deka S, Barthakur S (2010) Overview on current status of biotechnological interventions on yellow stem borer Scirpophaga incertulas (Lepidoptera: Crambidae) resistance in rice. Biotech Adv 1(28):70–81
• Duan X, Li X, Xue Q, Saad MAE, Xu D, Wu R (1996) Transgenic rice plants harboring an introduced potato proteinase inhibitor II gene are insect resistant. Nat Biotech 14(4):494–498
• Dunn BM (1989) Proteolytic enzymes: a practical approach. Oxford, IRL Press 57–81
• Edmonds H, Gatehouse L, Hilder V, Gatehouse J (1996) The inhibitory effects of cysteine protease inhibitor oryzacystatin on digestive protease and on larval survival and development of the southern corn root worm (Diabrotica undicimpunctata hawardi). Entomol Exp Appl 83–94
• Gholizadeh A, Santha IM, Kohenhrouz BB, Lodha ML, Kapoor HC (2005) Cystatin may confer viral resistance in plants by inhibition of a virus-induced cell death phenomenon in which cysteine protease active: cloning and molecular characterization of a cDNA encoding cysteine-protease inhibitor (celostatin) from Celosia cristata (Crested cook‘comb). Biotech Appl Biochem 42:197–204
• Hama H, Suzuki K, Tanaka H (1992) Inheritance and stability of resistance to Bacillus thuringiensis formulations in the diamondback moth Plutella xylostella (Linnaeus) (Lepidoptera: Yponomeuidae). J Appl Entomol Zool 27:355–362
• Heckel DG, Gahan LJ, Baxter SW, Zhao JZ, Shelton AM, Gould F, Tabashnik BE (2007) The diversity of Bt resistance genes in species of Lepidoptera. J Invert Pathol 95:192–197
• Hilder VA, Gatehouse AMR, Sheerman SE, Barker RF, Boulter D (1987) A novel mechanism of insect resistance engineered into tobacco. Nature 300:160–163
• Hines ME, Osuala CI, Nielson SS (1991) Isolation and partial characterization of a cystatin cysteine protease inhibitor of coleopteron digestive proteolytic activity. J Agric Food Chem 39:1515–1520
• Ho NH, Baisakh N, Oliva N, Datta K, Frutos R, Datta SK (2006) Translational fusion hybrid Bt genes confer resistance against yellow stem borer in transgenic elite Vietnamese rice (Oryza sativa L.) cultivars. Crop Sci 46:781–789
• Johnson R, Narvaez J, An G, Ryan C (1989) Expression of proteinase inhibitors I and II in transgenic tobacco plants: effects on natural defense against Manduca sexta larvae. Proc Nat Aca Sci 86:871–875
• Kulshreshtha JP, Nigam PM (1987) Integrated management of key pest of paddy. In: Mathur YK, Bhattaacharya B, Upadhyay ND, Srivastava JP (eds) Recent advances in entomology. New Gopal Printing Press Parade, Kanpur, pp 186–211
• Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685
• Laskowski MJ, Kato I, Ardelt W, Cook J, Denton A, Empie MW, Kohr WJ, Park SJ, Parks K, Schatzley BL (1987) Ovomucoid third domains from 100 avian species: isolation, sequences, and hyper variability of enzyme-inhibitor contact residues. Biochemistry 26:202–221
• Lowery OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the phenol reagent. J Biol Chem 193:265–275
• Markwick NP, Docherty LC, Phung MM, Lester MT, Murry C, Yao JL, Mitra DS, Cohen D, Beuunning LL, Kutty-Amma S, Christeller IT (2003) Transgenic tobacco and apple plants expressing biotin-binding proteins are resistant to two cosmopolitan insect pests, potato tuber moth and light brown apple moth, respectively. Transgene Res 12:671–681
• Michaud D, Nguyen-Quoc B, Vrain TC, Fong D, Yelle S (1996) Response of digestive cysteine proteinases from the Colorado potato beetle (Leptinotarsa decemlineata) and the black vine weevil (Otiorynchus sulcatus) to a recombinant form of human stefin A. Arch Insect Biochem Physiol 31:451–464
• Miska S, Kurodo M, Iwabuchi K, Abe K, Arai S (1996) Soyacystatin, a novel cysteine protease inhibitor in Soybean, district in protein structure and gene organization from other cystatins of animal and plant origin. J Biochem 290:609–614
• Motulsky HJ (1999) Analyzing data with GraphPad prism. GraphPad Software Inc, San Diego
• Murdock LL, Brookhart G, Dunn PE, Foard DE, Kelley S, Kitch L, Shade RE, Shukle RH, Wolfson JL (1987) Cysteine digestive proteinases in coleoptera. Comp Biochem Physiol 87B:783–787
• Nagata T, Kamimuro T, Wang YC, Han SG, Noor NM (2002) Recent status of insecticide resistance of long-distance migrating rice plant hoppers monitored in Japan, China, and Malaysia. J. Asia Pacific Entomol 5:113–116
• Nayak P, Basu D, Das S, Basu A, Ghosh D, Ramakrishnan NA, Ghosh M, Sen SK (1997) Transgenic elite indica rice plants expressing CryIAc q-endotoxin of Bacillus thuringiensis are
• resistant against yellow stem borer (Scirpophaga incertulas). Proc Natl Acad Sci 94:2111–2116
• Neto OBO, Batista JAN, Rigden DJ, Franco OL, Fragoso RR, Monteiro ACS, Monnerat RG, Grossi-de-sa MJ (2004) Molecular cloning of a cysteine proteinase cDNA from the cotton boll weevil Anthomus grandis (Coleopteran: Curculinonidae). Biosci Biotech Biochem 68(6):1235–1245
• Novillo C, Castanera P, Ortego F (1997) Inhibition of digestive trypsin-like proteases from larvae of several lepidopteran species by the diagnostic cysteine protease inhibitor E-64. Insect Biochem Mol Biol 27:625–638
• Oppert B, Morgan TD, Hartzer K, Lenarcic B, Galesa K, Brzin J, Turk V, Yoza K, Ohtsubo K, Kramer KJ (2003) Effects of proteinase inhibitors on digestive proteinases and growth of the red flour beetle, Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae). Comp Biochem Physiol 134:481–490
• Puzstai A, Grant G, Brown DJ, Stewart JC, Bardocz S, Ewen SWB, Gatehouse AMR, Hilder VA (1992) Nutritional evaluation of the trypsin (EC3.4.21.4) inhibitor from cowpea (Vigna unguiculata). Br J Nutr 68:783–791
• Schuler TH, Poppy GM, Kerry BR, Denholm I (1998) Insect resistant transgenic plants. Trends Biotech 16:168–175
• Sharma HC, Sharma KK, Seetaram N, Ortiz R (2000) Prospects for transgenic resistance to insects. Electr J Biotech 3(1):37–42
• Siddappa GS (1957) Protease inhibitors from jackfruit seed (Artocarpus integrifolia). Sci Ind Res 166
• Singh RR, Rao AG (2002) Reductive unfolding and oxidative refolding of a Bowman–Birk inhibitor from horsegram seeds (Dolichos biflorus): evidence for ‘hyper reactive’ disulfide bonds and rate-limiting nature of disulfide isomerization in folding. Biochem Biophys Acta 1597:280–291
• Tabashnik BE (1994) Evolution of resistance to Bacillus thuringiensis. Ann Rev Entomol 39:47–79
• Tabashnik BE, Carriere Y, Dennehy TJ, Morin S, Sisterson MS, Roush RT, Shelton AM, Zhao JZ (2003) Insect resistance to transgenic Bt crops: lessons from the laboratory and field. J Econ Entomol 96:1031–1038
• Terra WR, Ferreira C (1994) Insect digestive enzymes: properties, compartmentalization and function. Comp Biochem Physiol 109B:1–62
• Vain NP, Worland B, Clarke MC, Richard G, Beavis M, Liu H, Kohli A, Leech M, Snape J, Christou P (1998) Expression of an engineered cysteine proteinase inhibitor (Oryzacystatin-I delta D86) for nematode resistance in transgenic rice plants. Theor Appl Genet 96:266–271
• Walker AJ, Ford L, Majerus MEN, Eoghegan IE, Birch ANE, Gatehouse JA, Gatehouse AMR (1998) Characterisation of the midgut digestive proteinase activity of the two-spot ladybird (Adalia bipunctata L.) and its sensitivity to proteinase inhibitors. Insect Biochem Mol Biol 28:173–180
• Wilhite SE, Elden TC, Brzin J, Smigocki AC (2000) Inhibition of cysteine and aspartyl proteinases in the alfalfa weevil midgut with biochemical and plant-derived proteinase inhibitors. Insect Biochem Mol Bio 30:1181–1188

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