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2014 | 36 | 05 |

Tytuł artykułu

Transcriptional regulation of Arabidopsis thaliana WRKY genes under interaction with beneficial fungus Trichoderma atroviride

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
Plants are associated with a wide range of microorganisms, and these interactions induce changes in both the plant and microorganism. Transcription factors play an important role in the regulation of large numbers of genes associated to plant–microbe response. WRKY transcription factors have been involved in the responses to plant–pathogen interactions, but little is known about WRKY transcription factors in beneficial plant–microbe interactions. In this study, the expression patterns of Arabidopsis thaliana WRKY genes were evaluated during the interaction with the beneficial fungus Trichoderma atroviride. Eight WRKY genes, AtWRKY8, AtWRKY33, AtWRKY38, AtWRKY42, AtWRKY54, AtWRKY57, AtWRKY60 and AtWRKY70, were analyzed by quantitative RT-PCR. These WRKY genes were found differentially expressed in a time-dependent manner during T. atroviride interaction. Our data suggest that T. atroviride induces the expression of positive regulators in jasmonic acid-mediated pathway such as AtWRKY8, AtWRKY33, AtWRKY38, AtWRKY42 and AtWRKY60, while salicylic acid pathway regulated by AtWRKY70 and AtWRKY54, could be activated at later stages of the interaction, when the fungus is fully established in the plant roots. In addition, Trichoderma treatment regulates the expression of WRKY genes such as AtWRKY57, AtWRKY60 and AtWRKY33 related to response to abiotic stresses. In this sense, WRKY transcription factors regulation suggests a complex signaling network in this beneficial plant–microbe interaction.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

36

Numer

05

Opis fizyczny

p.1085-1093,fig.,ref.

Twórcy

  • Facultad de Ciencias Biologicas, Universidad Juarez del Estado de Durango, Av. Universidad S/N, Fracc. Filadelfia, C.P. 35010 Gomez Palacio, Durango, Mexico
  • Division de Biologia Molecular, Instituto Potosino de Investigacion Cientifica y Tecnologica, Camino a la Presa de San Jose 2055, Apartado Postal 3-74 Tangamanga, C.P. 78216 San Luis Potosi, Mexico
  • Division de Biologia Molecular, Instituto Potosino de Investigacion Cientifica y Tecnologica, Camino a la Presa de San Jose 2055, Apartado Postal 3-74 Tangamanga, C.P. 78216 San Luis Potosi, Mexico

Bibliografia

  • Agarwal P, Reddy MP, Chikara J (2011) WRKY: its structure, evolutionary relationship, DNA-binding selectivity, role in stress tolerance and development of plants. Mol Biol Rep 38:3883–3896
  • An YQ, McDowell JM, Huang S, McKinney EC, Chambliss S, Meagher RB (1996) Strong, constitutive expression of the Arabidopsis ACT2/ACT8 actin subclass in vegetative tissues. Plant J 10:107–121
  • Andreasson E, Jenkins T, Brodersen P, Thorgrimsen S, Petersen NHT, Zhu S, Qiu JL, Micheelsen P, Rocher A, Petersen M, Newman MA, Nielsen HB, Hirt H, Somssich I, Mattsson O, Mundy J (2005) The MAP kinase substrate MKS1 is a regulator of plant defense responses. EMBO J 24:2579–2589
  • Bae H, Sicher RC, Kim MS, Kim SH, Strem MD, Melnick RL, Bryan AB (2009) The beneficial endophyte Trichoderma hamatum isolate DIS 219b promotes growth and delays the onset of the drought response in Theobroma cacao. J Exp Bot 60:3279–3295
  • Besseau S, Li J, Palva ET (2012) WRKY54 and WRKY70 co-operate as negative regulators of leaf senescence in Arabidopsis thaliana. J Exp Bot 63:2667–2679
  • Brotman Y, Landau U, Cuadros-Inostroza A, Takayuki T, Fernie AR, Chet I, Viterbo A, Willmitzer L (2013) Trichoderma–plant root colonization: escaping early plant defense responses and activation of the antioxidant a machinery for saline stress tolerance. PLoS Pathog 9:e1003221
  • Champion A, Jouannic S, Guillon S, Mockaitis K, Krapp A, Picaud A, Simanis V, Kreis M, Henry Y (2004) AtSGP1, AtSGP2 and MAP4Ka are nucleolar plant proteins that can complement fission yeast mutants lacking a functional SIN pathway. J Cell Sci 117:4265–4275
  • Charrier B, Champion A, Henry Y, Kreis M (2002) Expression profiling of the whole Arabidopsis shaggy-like kinase multigene family by real-time reverse transcriptase-polymerase chain reaction. Plant Physiol 130(2):577–590
  • Chen YF, Li LQ, Xu Q, Kong YH, Wang H, Wu WH (2009) The WRKY6 transcription factor modulates PHOSPHATE1 expression in response to low Pi stress in Arabidopsis. Plant Cell 21:3554–3566
  • Chen H, Lai Z, Shi J, Xiao Y, Chen Z, Xu X (2010a) Roles of Arabidopsis WRKY18, WRKY40 and WRKY60 transcription factors in plant responses to abscisic acid and abiotic stress. BMC Plant Biol 10:281
  • Chen L, Lai Z, Yu D (2010b) Wounding-induced WRKY8 is involved in basal defense in Arabidopsis. Mol Plant Microbe Interact 23:558–565
  • Contreras-Cornejo HE, Macías-Rodríguez L, Cortés-Penagos C, López-Bucio J (2009) Trichoderma virens, a plant beneficial fungus, enhances biomass production and promotes lateral root growth through an auxin-dependent mechanism in Arabidopsis. Plant Physiol 149:1579–1592
  • Contreras-Cornejo HE, Macías-Rodríguez L, Beltrán-Peña E, Herrera-Estrella A, López-Bucio J (2011) Trichoderma-induced plant immunity likely involves both hormonal and camalexin dependent mechanisms in Arabidopsis thaliana and confers resistance against necrotrophic fungi Botrytis cinerea. Plant Signal Behav 6:1554–1563
  • Dana MM, Pintor-Toro JA, Cubero B (2006) Transgenic tobacco plants overexpressing chitinases of fungal origin show enhanced resistance to biotic and abiotic stress agents. Plant Physiol 142:722–730
  • Delgado-Sánchez P, Ortega-Amaro MA, Rodríguez-Hernández AA, Jiménez-Bremont JF, Flores J (2010) Further evidence from the effect of fungi on breaking opuntia seed dormancy. Plant Signal Behav 5:1229–1230
  • Dong J, Chen C, Chen Z (2003) Expression profiles of the Arabidopsis WRKY gene superfamily during plant defense response. Plant Mol Biol 51:21–37
  • Donoso EP, Bustamante RO, Caru M, Niemeyer HM (2008) Water deficit as a driver of the mutualistic relationship between the fungus Trichoderma harzianum and two wheat genotypes. Appl Environ Microbiol 74:1412–1417
  • Eulgem T, Somssich IE (2007) Networks of WRKY transcription factors in defense signaling. Curr Opin Plant Biol 10:366–371
  • Eulgem T, Rushton PJ, Robatzek S, Somssich IE (2000) The WRKY superfamily of plant transcription factors. Trends Plant Sci 5:199–206
  • Harman GE, Howell CR, Viterbo A, Chet I, Lorito M (2004) Trichoderma species––opportunistic, avirulent plant symbionts. Nat Rev Microbiol 2:43–56
  • Hermosa R, Viterbo A, Chet I, Monte E (2012) Plant-beneficial effects of Trichoderma and of its genes. Microbiology 158:17–25
  • Jiang Y, Deyholos MK (2009) Functional characterization of Arabidopsis NaCl-inducible WRKY25 and WRKY33 transcription factors in abiotic stresses. Plant Mol Biol 69:91–105
  • Jiang Y, Lianga G, Yu D (2012) Activated expression of WRKY57 confers drought tolerance in Arabidopsis. Mol Plant 5:1375–1388
  • Journot-Catalino N, Somssich IE, Roby D, Kroj T (2006) The transcription factors WRKY11 and WRKY17 act as negative regulators of basal resistance in Arabidopsis thaliana. Plant cell 18:3289–3302
  • Kim KC, Lai Z, Fan B, Chen Z (2008) Arabidopsis WRKY38 and WRKY62 transcription factors interact with histone deacetylase 19 in basal defense. Plant Cell 20:2357–2371
  • Korolev N, David DR, Elad Y (2008) The role of phytohormones in basal resistance and Trichoderma-induced systemic resistance to Botrytis cinerea in Arabidopsis thaliana. Biocontrol 53:667–683
  • Lai Z, Li Y, Wang F, Cheng Y, Fan B, Yu JQ, Chena Z (2011) Arabidopsis sigma factor binding proteins are activators of the WRKY33 transcription factor in plant defense. Plant Cell 23:3824–3841
  • Li J, Brader G, Palva ET (2004) The WRKY70 transcription factor: a node of convergence for jasmonate-mediated and salicylate-mediated signals in plant defense. Plant Cell 16:319–331
  • Li J, Brader G, Kariola T, Palva ET (2006) WRKY70 modulates the selection of signaling pathways in plant defense. Plant J 46:477–491
  • Li S, Fu Q, Chen L, Huang W, Yu D (2011) Arabidopsis thaliana WRKY25, WRKY26, and WRKY33 coordinate induction of plant thermotolerance. Planta 233:1237–1252
  • Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2(-delta delta C(T)) method. Methods 25:402–408
  • Lorito M, Woo SL, Harman GE, Monte E (2010) Translational research on Trichoderma: from ‘omics to the field. Annu Rev Phytopathol 48:395–417
  • Maeo K, Hayashi S, Kojima-Suzuki H, Morikami A, Nakamura K (2001) Role of conserved residues of the WRKY domain in the DNA-binding of tobacco WRKY family proteins. Biosci Biotechnol Biochem 65:2428–2436
  • Mastouri F, Björkman T, Harman GE (2010) Seed treatment with Trichoderma harzianum alleviates biotic, abiotic, and physiological stresses in germinating seeds and seedlings. Phytopathology 100:1213–1221
  • Morán-Diez E, Rubio B, Dominguez S, Hermosa R, Monte E, Nicolas C (2012) Transcriptomic response of Arabidopsis thaliana after 24 h incubation with the biocontrol fungus Trichoderma harzianum. J Plant Physiol 169:614–620
  • Pandey SP, Somssich IE (2009) The role of WRKY transcription factors in plant immunity. Plant Physiol 150:1648–1655
  • Rushton PJ, Somssich IE, Ringler P, Shen QJ (2010) WRKY transcription factors. Trends Plant Sci 15:247–258
  • Sáenz-Mata J, Jiménez-Bremont JF (2012) HR4 gene is induced in the Arabidopsis–Trichoderma atroviride beneficial interaction. Int J Mol Sci 13:9110–9128
  • Salas-Marina MA, Silva-Flores MA, Uresti-Rivera EE, Castro-Longoria E, Herrera-Estrella A, Casas-Flores S (2011) Colonization of Arabidopsis roots by Trichoderma atroviride promotes growth and enhances systemic disease resistance through jasmonic acid/ethylene and salicylic acid pathways. Eur J Plant Pathol 131:15–26
  • Segarra G, Casanova E, Bellido D, Odena M, Oliveira E, Trillas E (2007) Proteome, salicylic acid, and jasmonic acid changes in cucumber plants inoculated with Trichoderma asperellum strain T34. Proteomics 7:3943–3952
  • Shoresh M, Harman GE, Mastouri F (2010) Induced systemic resistance and plant responses to fungal biocontrol agents. Annu Rev Phytopathol 48:21–43
  • Sukumar P, Legué V, Vayssières A, Martin F, Tuskan GA, Kalluri UC (2013) Involvement of auxin pathways in modulating root architecture during beneficial plant–microorganism interactions. Plant Cell Environ 36:909–919
  • Tucci M, Ruocco M, De Masi L, De Palma M, Lorito M (2011) The beneficial effect of Trichoderma spp. on tomato is modulated by the plant genotype. Mol Plant Pathol 12:341–354
  • Ülker B, Somssich IE (2004) WRKY transcription factors: from DNA binding towards biological function. Curr Opin Plant Biol 7:491–498
  • Van Verk MC, Bol JF, Linthorst HJM (2011) Prospecting for genes involved in transcriptional regulation of plant defense, a bioinformatics approach. BMC Plant Biol 11:1–12
  • Wang D, Amornsiripanitch N, Dong X (2006) A genomic approach to identify regulatory nodes in the transcriptional network of systemic acquired resistance in plants. PLoS Pathog 2:e123
  • Wang Q, Wang M, Zhang X, Hao B, Kaushik SK, Pan Y (2011) WRKY gene family evolution in Arabidopsis thaliana. Genetica 139:973–983
  • Xu X, Che NC, Fan B, Chen Z (2006) Physical and functional interactions between pathogen-induced Arabidopsis WRKY18, WRKY40, and WRKY60 transcription factors. Plant Cell 18:1310–1326
  • Yamamoto S, Nakano T, Suzuki K, Shinshi H (2004) Elicitor-induced activation of transcription via W box-related cis-acting elements from a basic chitinase gene by WRKY transcription factors in tobacco. Biochim Biophys Acta 18(3):279–287
  • Yedidia I, Srivastva AK, Kapulnik Y, Chet I (2001) Effect of Trichoderma harzianum on microelement concentrations and increased growth of cucumber plants. Plant Soil 235:235–242
  • Yoshioka Y, Ichikawa H, Naznin HA, Kogure A, Hyakumachi M (2012) Systemic resistance induced in Arabidopsis thaliana by Trichoderma asperellum SKT-1, a microbial pesticide of seedborne diseases of rice. Pest Manag Sci 68:60–66
  • Zhang Y, Wang L (2005) The WRKY transcription factor superfamily: its origin in eukaryotes and expansion in plants. BMC Evol Biol 5:1–12
  • Zheng Z, Qamar SA, Chen Z, Mengiste T (2006) Arabidopsis WRKY33 transcription factor is required for resistance to necrotrophic fungal pathogens. Plant J 48:592–605

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

bwmeta1.element.agro-9c6918ae-e7e9-48b7-b524-c5dcf654de98
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