Modulation in growth, photosynthetic efficiency, activity of antioxidants and mineral ions by foliar application of glycinebetaine on pea (Pisum sativum L.) under salt stress

• Autorzy: Nusrat N. , Shahbaz M. , Perveen S.
• Języki publikacji: EN

Abstrakty

EN

A pot experiment was carried out to explore the role of glycinebetaine (GB) as foliar spray foliar on two pea (Pisum sativum L.) varieties (Pea 09 and Meteor Fsd) under saline and non-saline conditions. Thirty-two-day-old plants were subjected to two levels 0 and 150 mM of NaCl stress. Salt treatment was applied in full strength Hoagland’s nutrient solution. Three levels 0, 5 and 10 mM of GB were applied as foliar treatment on 34-day-old pea plants. After 2 weeks of foliar treatment with GB data for various growth and physiochemical attributes were recorded. Rooting-medium applied salt (150 mM NaCl) stress decreased growth, photosynthesis, chlorophyll, chlorophyll fluorescence and soluble protein contents, while increasing the activities of enzymatic (POD and CAT) and nonenzymatic (ascorbic acid and total phenolics) antioxidant enzymes. Foliar application of GB decreased root and shoot Na⁺ under saline conditions, while increasing shoot dry matter, root length, root fresh weight, stomatal conductance (gₛ), contents of seed ascorbic acid, leaf phenolics, and root and shoot Ca²⁺ contents. Of three GB (0, 5, 10 mM) levels, 10 mM proved to be more effective in mitigating the adverse effects of salinity stress. Overall, variety Pea 09 showed better performance in comparison to those of var. Meteor Fsd under both normal and salinity stress conditions. GB-induced modulation of seed ascorbic acid, leaf phenolics, gₛ, and root Ca²⁺ values might have contributed to the increased plant biomass, reduction of oxidative stress, increased osmotic adjustment and better photosynthetic performance of pea plants under salt stress.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2014
• Tom: 36
• Numer: 11
• Opis fizyczny: p.2985-2998,fig.,ref.

Twórcy

autor

Nusrat N.

• Department of Botany, University of Agriculture, Faisalabad, Pakistan

autor

Shahbaz M.

• Department of Botany, University of Agriculture, Faisalabad, Pakistan

autor

Perveen S.

• Department of Botany, Government College University, Faisalabad, Pakistan

Bibliografia

• Abbas SR, Ahmad SD, Sabir SMY, Shah AH (2014) Detection of drought tolerant sugarcane genotypes (Saccharum officinarum) using lipid peroxidation, antioxidant activity, glycine-betaine and proline contents. J Soil Sci Plant Nutr 14(1):233–243
• Ahmad P, Hakeem KR, Kumar A, Ashraf M, Akram NA (2012) Salt-induced changes in photosynthetic activity and oxidative defense system of three cultivars of mustard (Brassica juncea L.). Afr J Biotechnol 11:2694–2703
• Akhter N, Akram NA, Shahbaz M (2007) Presowing seed treatments with glycinebetaine and mineral nutrients of wheat (Triticum aestivum L.) under saline conditions. Pak J Agric Sci 44(2):236–241
• Ali RM, Kaviani B, Masouleh A (2012) The effect of exogenous glycine betaine on yield of soybean [Glycine max (L.) Merr.] in two contrasting cultivars Pershing and DPX under soil salinity stress. Plant Omics, Southern Cross Publisher
• Allen SK, Dobrenz AK, Schonhorst MH, Stoner JE (1985) Heritability of NaCl tolerance in germinating alfalfa seeds. Agron J 77:90–96
• Arnon DT (1949) Copper enzyme in isolated chloroplasts polyphenoloxidase in Beta vulgaris. Plant Physiol 24:1–15
• Ashraf M, Foolad MR (2007) Roles of glycine betaine and proline in improving plant abiotic stress resistance. Environ Exp Bot 59:206–216
• Ashraf M, Harris PJC (2004) Potential biochemical indicators of salinity tolerance in plants. Plant Sci 166:3–16
• Ashraf I, Pervez MA, Mand A, Ahmad R (2011) Effect of varying irrigation frequencies on growth, yield and quality of peas seed. J Agric Res 49(3):339–351
• Ashraf MA, Ashraf M, Shahbaz M (2012) Growth stage-based modulation in antioxidant defense system and proline accumulation in two hexaploid wheat (Triticum aestivum L.) cultivars differing in salinity tolerance. Flora 207(5):388–397
• Basu PS, Sharma A, Sukumaran NP (1998) Changes in net photosynthetic rate and chlorophyll fluorescence in potato leaves induced by water stress. Photosynthetica 35:13–19
• Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Ann Biochem 72:248–254
• Brugnoli E, Bjorkman O (1992) Growth of cotton under continuous salinity stress influence on allocation pattern, stomatal and non stomatal components of photosynthesis and dissipation of excess light energy. Planta 187:335–347
• Chance B, Maehly A (1955) Assay of catalase and peroxidase. Methods Enzymol 2:764–817
• Cha-Um S, Kirdmanee C (2010) Effect of glycinebetaine on proline, water use, and photosynthetic efficiencies, and growth of rice seedlings under salt stress. Turk J Agric For 34:517–527
• Cha-um S, Samphumphuang T, Kirdmanee C (2013) Glycinebetaine alleviates water deficit stress in indica rice using proline accumulation, photosynthetic efficiencies, growth performances and yield attributes. Aus J Crop Sci 7(2):213–218
• Chen THH, Murata N (2002) Enhancement of tolerance to abiotic stress by metabolic engineering of betaines and other compatible solutes. Curr Opin Plant Biol 5:250–257
• Chen THH, Murata N (2011) Glycinebetaine protects plants against abiotic stress: mechanisms and biotechnological applications. Plant Cell Environ 34:1–20
• Chen S, Gollop N, Heuer B (2009) Proteomic analysis of salt-stressed tomato (Solanum lycopersicum) seedlings: effect of genotype and exogenous application of glycinebetaine. J Exp Bot, erp075v1–erp075
• Cramer GR, Nowak RS (1992) Supplemental manganese improves the relative growth, net assimilation and photosynthetic rates of salt-stressed barley. Physiol Plant 84(4):600–605
• Cui XH, Fu-Shun H, Chen H, Chen J, Wang XC (2008) Expression of the Vicia faba VfPIP gene in Arabidopsis thaliana plants improves there drought resistance. J Plant Res 121:207–214
• De Pascale E, Ruggiero C, Barbieri G, Maggio A (2003a) Physiological responses of pepper to salinity and drought. J Am Soc Hortic Sci 128:48–54
• De Pascale S, Maggio A, Ruggiero C, Barbieri G (2003b) Growth, water relations, and ion content of field grown celery under saline irrigation (Apium graveolens L. var. dulce [Mill.] pers.). J Am Soc Hortic Sci 128:136–143
• Demiral T, Turkan I (2004) Does exogenous glycinebetaine affect antioxidative system of rice seedlings under NaCl treatment. J Plant Physiol 161:1089–1100
• Gadallah MAA (1999) Effect of proline and glycinebetaine on Vicia faba responses to salt stress. Biol Plant 42:249–257
• Giannopolitis CN, Ries SK (1977) Superoxide dismutase. I. Occurrence in higher plants. Plant Physiol 59:309–314
• Giridarakumar S, Matta Reddy A, Sudhakar C (2003) NaCl effects on proline metabolism in two high yielding genotypes of mulberry (Morus alba L.) with contrasting salt tolerance. Plant Sci 165:1245–1251
• Girija C, Smith BN, Swamy PM (2002) Interactive effects of sodium chloride and calcium chloride on the accumulation of proline and glycinebetaine in peanut (Arachis hypogaea L.). Environ Exp Bot 47:1–10
• Grieve CM, Maas EM (1984) Betaine accumulation in salt stressed sorghum. Physiol Plant 61:167–171
• Habib N, Ashraf M, Shahbaz M (2013) Effect of exogenously applied nitric oxide on some key physiological attributes of rice (Oryza sativa L.) plants under salt stress. Pak J Bot 45(5):1563–1569
• Hassine AB, Ghanem ME, Bouzid S, Lutts S (2008) An inland and a coastal population of the Mediterranean xero-halophyte species Atriplex halimus L. differ in their ability to accumulate proline and glycinebetaine in response to salinity and water stress. J Exp Bot 59:1315–1326
• Heuer B (2003) Influence of exogenous application of proline and glycinebetaine on growth of salt-stressed tomato plants. Plant Sci 165:693–699
• Hoque MA, Okuma E, Nakamara Y, Shimoishi Y, Murata Y (2008) Proline and glycinebetaine enhance antioxidant defense and methylglyoxal detoxification systems and reduce NaCl induced damage in cultured tobacco cells. J Plant Physiol 165:813–824
• Hussein MM, El-Gereadly NHM, El-Desuki M (2006) Role of puterscine in resistance to salinity of pea plants (Pisum sativum L.). J Appl Sci Res 2:598–604
• Ibrahim M, Anjum A, Khaliq N, Iqbal M, Athar HR (2006) Four foliar applications of glycinebetaine did not alleviate adverse effects of salt stress on growth of sunflower. Pak J Bot 38(5):1561–1570
• Javaid A, Ghafoor Anwar R (2002) Evaluation of local and exotic pea (Pisum sativum) germplasm for vegetative and dry grain traits. Pak J Bot 34(4):419–427
• Julkenen-Titto R (1985) Phenolic constituents in the leaves of northern willows: methods for the analysis of certain phenolics. Agric Food Chem 33:213–217
• Kalaji HM, Govindje E, Bosa K, Koscielniak J, Zuk-Golaszewska K (2010) Effects of salt stress on photosystem II efficiency and CO₂ assimilation of two Syrian barley landraces. Environ Exp Bot 73:64–72
• Kanwal H, Ashraf M, Shahbaz M (2011) Assessment of salt tolerance of some newly developed and candidate wheat (Triticum aestivum L.) cultivars using gas exchange and chlorophyll fluorescence attributes. Pak J Bot 43(6):2693–2699
• Kanwal S, Ashraf M, Shahbaz M, Iqbal MY (2013) Influence of saline stress on growth, gas exchange, mineral nutrients and nonenzymatic antioxidatns in mungbean [(Vigna radiata (L.) Wilczek]. Pak J Bot 45(3):763–771
• Karabudak T, Bor M, Ozdemir F, Turkan I (2014) Glycine betaine protects tomato (Solanum lycopersicum) plants at low temperature by inducing fatty acid desaturase7 and lipoxygenase gene expression. Mol Biol Rep 41(3):1401–1410
• Kausar F, Shahbaz M (2013) Interactive effect of foliar application of nitric oxide (NO) and salinity on wheat (Triticum aestivum L.). Pak J Bot 45(SI):67–73
• Kausar F, Shahbaz M, Ashraf M (2013) Protective role of foliar-applied nitric oxide in wheat (Triticum aestivum L.) under saline stress. Turk J Bot 37(6):1155–1165
• Kausar N, Nawaz K, Hussain K, Bhatti KH, Siddiqi EH, Tallat A (2014) Effect of exogenous applications of glycine betaine on growth and gaseous exchange attributes of two maize (Zea mays L.) cultivars under saline conditions. World Appl Sci J 29:1559–1565
• Kaya C, Sonmez O, Aydemir S, Dilkilitas M (2013) Mitigation effects of glycinebetaine on oxidative stress and some key growth parameters of maize exposed to salt stress. Turk J Agric 37:188–194
• Khan TN, Ramzan A, Jillani G, Mehmood T (2013) Morphological performance of peas (Pisum sativum) genotypes under rainfed conditions of potowar region. J Agric Res 51(1):51–60
• Khan MIR, Asgher M, Khan NA (2014) Alleviation of salt-induced photosynthesis and growth inhibition by salicylic acid involves glycinebetaine and ethylene in mungbean (Vigna radiata L.). Plant Physiol Biochem 80:67–74
• Lopez CML, Takahashi H, Yamazaki S (2002) Plant water relations of kidney bean plants treated with NaCl and foliarly applied glycinebetaine. J Agron Crop Sci 188:73–80
• Mahajan S, Tuteja N (2005) Cold, salinity and drought stresses: an overview. Arch Biochem Biophys 444:139–158
• Makela P, Kontturi M, Pheu E, Somersalo S (1999) Photosynthetic response of drought and salt-stressed tomato and turnip rape plants to foliar-applied glycinebetaine. Physiol Plant 105:45–50
• Makela P, Karkkainen J, Somersalo S (2000) Effect of glycinebetaine on chloroplast ultrastructure, chlorophyll and protein content, and RuBPCO activities in tomato grown under drought or salinity. Biol Plant 43:471–475
• Makhdum MI, Shababuddin MI (2006) Effects of different doses of glycine betaine and time of spray application on yield of cotton (Gossypium hirsutum L.). J Res (Science) 17(4):241–245
• McCue RF, Hanson AD (1990) Drought and salt tolerance: towards understanding and application. TIBTECH 8:358–362
• Meek CR, Oosterhuis DM (1999) Effects of foliar application of glycinebetaine on fieldgrown cotton. In: Oosterhuis DM (ed) Proc. 1999 cotton research meeting and summaries of research in progress. University of Arkansas Agricultural Experiment Station Special, Report 193, pp 103–105
• Meek C, Oosterhuis D, Gorham J (2003) Does foliar-applied glycinebetaine affect endogenous betaine levels and yield in cotton? Crop Manage. doi:10.1094/CM-2003-0804-02-RS(Online
• Moghaieb REA, Saneoka H, Fujita K (2004) Effect of salinity on osmotic adjustment, glycinebetaine accumulation and the betaine aldehyde dehydrogenase gene expression in two halophytic plants, Salicornia europaea and Suaeda maritima. Plant Sci 166:1345–1349
• Morales F, Abadia A, Gomez-Aparis J, Abadia J (1992) Effects of combined NaCl and CaCl₂ salinity on photosynthetic parameters of barley grown in nutrient solution. Physiol Plant 86:419–426
• Mukherjee SP, Choudhuri MA (1983) Implications of water stressinduced changes in the levels of endogenous ascorbic acid and hydrogen peroxide in Vigna seedlings. Physiol Plant 58:166–170
• Munns R, Tester M (2008) Mechanisms of salinity tolerance. Annu Rev Plant Biol 59:651–681
• Murata N, Mohanty PS, Hayashi H, Papageorgiou GC (1992) Glycinebetaine stabilizes the association of extrinsic proteins with the photosynthetic oxygen evolving complex. FEBS Lett 296:187–189
• Navarro J, Flores M, Garrido PC, Martinez V (2006) Changes in the contents of antioxidant compounds in pepper fruits at different ripening stages, as affected by salinity. Food Chem 96:66–73
• Nawaz K, Ashraf M (2010) Exogenous application of glycinebetaine modulates activities of antioxidants in maize plants subjected to salt stress. J Agron Crop 196:28–37
• Nomura M, Hibino T, Takabe T, Sugiyama T, Yokota A, Miyake H, Takabe T (1998) Transgenically produced glycinebetaine protects ribulose 1,5-bisphosphate carboxylase/oxygenase from inactivation in Synechococcus sp. PCC7942 under salt stress. Plant Cell Physiol 39:425–432
• Noreen Z, Ashraf M (2009) Assessment of variation in antioxidative defense system in salt- treated pea (Pisum sativum) cultivars and its putative use as salinity tolerance markers. J Plant Physiol 166:1764–1774
• Odjegba VJ (2013) Responses of Zea mays seedlings to salinity stress and exogenous nitrogen supply. Nat Sci 11(1):63–69
• Perveen S, Shahbaz M, Ashraf M (2010) Regulation in gas exchange and quantum yield of photosystem II (PSII) in salt stressed and non-stressed wheat plants raised from seed treated with triacontanol. Pak J Bot 42:3073–3081
• Perveen S, Shahbaz M, Ashraf M (2011) Modulation in activities of antioxidant enzymes in salt stressed and non-stressed wheat (Triticum aestivum L.) plants raised from seed treated with triacontanol. Pak J Bot 43(5):2463–2468
• Perveen S, Shahbaz M, Ashraf M (2012a) Is pre-sowing seed treatment with triacontanol effective in improving some physiological and biochemical attributes of wheat (Triticum aestivum L.) under salt stress? J Appl Bot Food Qual 85:41–48
• Perveen S, Shahbaz M, Ashraf M (2012b) Changes in mineral composition, uptake and use efficiency of salt stressed wheat (Triticum aestivum L.) plants raised from seed treated with triacontanol. Pak J Bot 44:27–35
• Perveen S, Shahbaz M, Ashraf M (2013) Influence of foliar-applied triacontanol on growth, gas exchange characteristics, and chlorophyll fluorescence at different growth stages in wheat under saline conditions. Photosynthetica 51(4):541–551
• Perveen S, Shahbaz M, Ashraf M (2014) Triacontanol-induced changes in growth, yield, leaf water relations, antioxidative defense system and some key osmoprotectants in bread wheat (Triticum aestivum L.) under saline stress. Turk J Bot 38:896–913
• Raza SH, Athar HR, Ashraf M (2006) Influence of exogenously applied glycinebetaine on the photosynthetic capacity of two differently adapted wheat cultivars under salt stress. Pak J Bot 38(2):341–351
• Raza SH, Athar HR, Ashraf M, Hameed A (2007) GB-induced modulation of antioxidant enzymes activities and ion accumulation in two wheat cultivars differing in salt tolerance. Env Exp Bot 60:368–376
• Reddy KR, Henry WB, Seepaul R, Lokhande S, Gajanayake B, Brand D (2013) Exogenous application of glycinebetaine facilitates maize (Zea mays L) growth under water deficit conditions. Am J Exp Agric 3(1):1–13
• Rhodes D, Hanson AD (1993) Quaternary ammonium and tertiary sulfonium compounds in higher plants. Annu Rev Plant Physiol Plant Mol Biol 44:357–384
• Rhodes DP, Rich J, Myers AC, Rueter CC, Jamieson GC (1987) Determination of betaines by fast atom bombardment mass spectrometry: identification of glycinebetaine deficient genotypes of Zea mays. Plant Physiol 84:781–788
• Robinson SP, Jones JP (1986) Accumulation of glycinebetaine in chloroplasts provides osmotic adjustment during salt stress. Aust J Plant Physiol 13:659–668
• Rodriguez M, Canales E, Borras-Hidalgo O (2005) Molecular aspects of abiotic stress in plants. Biotecnologia Aplicada 22:1–10
• Sakamoto H, Murata N (2000) Genetic engineering of glycinebetaine synthesis in plants: current status and implications for enhancement of stress tolerance. J Exp Bot 51:81–88
• Sakr MT, El-Sarkassy NM, Fuller MP (2012) Osmoregulators proline and glycine betaine counteract salinity stress in canola. Agron Sus Dev 32(3):747–754
• Shahbaz M, Ashraf M (2013) Improving salinity tolerance in cereals. Crit Rev Plant Sci 32:237–249
• Shahbaz M, Zia B (2011) Does exogenous application of glycinebetaine through rooting medium alter rice (Oryza sativa L.) mineral nutrient status under saline conditions? J Appl Bot Food Qual 84(1):54–60
• Shahbaz M, Masood Y, Perveen S, Ashraf M (2011) Is foliar-applied glycinebetaine effective in mitigating the adverse effects of drought stress on wheat (Triticum aestivum L.)? J Appl Bot Food Qual 84:192–199
• Shahbaz M, Ashraf M, Al-Qurainy F, Harris PJC (2012) Salt tolerance in selected vegetable crops. Crit Rev Plant Sci 31(4):303–320
• Shahbaz M, Noreen N, Perveen S (2013) Triacontanol modulates photosynthesis and osmoprotectants in canola (Brassica napus L) under saline stress. J Plant Inter 8(4):250–259
• Shaheen HL, Shahbaz S, Ullah I, Iqbal MZ (2012) Morphophysiological responses of cotton (Gossypium hirsutum L.) to salt stress. Int J Agric Biol 14:980–984
• Shahid MA, Pervez MA, Ashraf MY, Ayyub CM, Ashfaq M, Mattson NS (2011) Characterization of salt tolerant and salt sensitive pea (Pisum sativum L.) genotypes under saline regime. Pak J Life Soc Sci 9(2):145–152
• Shahid MA, Pervez MA, Balal RM, Abbas T, Ayyub CM, Mattson NS, Riaz A, Iqbal Z (2012) Screening of pea (Pisum sativum L.) genotypes for salt tolerance based on early growth stage attributes and leaf inorganic osmolytes. Aust J Crop Sci 6(9):1324–1331
• Snedecore GW, Cohran WG (1980) Statistical methods, 7th edn. The Iowa State University Press, Ames
• Strasser RJ, Srivastava A, Govindjee (1995) Polyphasic chlorophyll ‘a’ fluorescence transients in plants and cyanobacteria. Photochem Photobiol 61:32–42
• Subbarao GV, Wheeler RM, Levine LH, Stutte GW (2001) Glycinebetaine accumulation, ionic and water relations of red-beet at contrasting levels of sodium supply. J Plant Physiol 158:767–776
• Timasheff SN (1992) A physicochemical basis for the selection of osmolytes by nature. In: Somero CN, Osmond CB, Bolis CL (eds) Water and life: comparative analysis of water relationships at the organismic, cellular and molecular levels. Springer, Berlin, pp 71–84
• Varshney KA, Gangwar LP, Goel N (1988) Choline and betaine accumulation in Trifolium alexandrinum L. during salt stress. Egyptian J Bot 31:81–86
• Wakeel A, Asif AR, Pitann B, Schubert S (2011) Proteome analysis of sugar beet (Beta vulgaris L.) elucidates constitutive adaptation during the first phase of salt stress. J Plant Physiol 168:519–526
• Wilson S (2001) Frost management in cool climate vineyards. Final Report to Grape and Wine Research & Development Corporation, Australia
• Wyn Jones RG, Gorham J, McDonnell E (1984) Organic and inorganic solute contents as selection criteria for salt tolerance in the Triticeae. In: Staples R, Toennissen GH (eds) Salinity tolerance in plants: strategies for crop improvement. Wiley, New York, pp 189–203
• Yang X, Lu C (2005) Photosynthesis is improved by exogenous glycinebetaine in salt stressed maize plants. Physiol Plant 124(3):343–352
• Yildiztugay E, Ozfidan-Konakci C, Kucukoduk M, Duran Y (2014) Variations in osmotic adjustment and water relations of Sphaerophysa kotschyana: glycine betaine, proline and choline accumulation in response to salinity. Bot Stud 55:6
• Zaidi MA, Amjad N, Shah S (2013) A study for the development of a vegetable planter for optimum stand establishment. Pak J Agric Sci 50(2):273–277
• Zeid IM (2009) Trehalose as osmoprotectant for maize under salinity-induced stress. Res J Agric Biol Sci 5:613–622
• Zhu MY, Ahn SJ, Matsumoto H (2003) Inhibition of growth and development of root border cells by Al. Physiol Plant 117:359–367

Identyfikatory

DOI

10.1007/s11738-014-1670-1