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2017 | 66 | 2 |

Tytuł artykułu

Bactericidal activity of cctenidine to various genospecies of Borrelia burgdorferi, sensu lato spirochetes in vitro and in vivo

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
The aim of our studies was to invent a reliable method for detection of the bactericidal activity of disinfectants against Borrelia burgdorferiin suspension (in vitro) and in cell line cultures (in vivo). In the suspension method, 0.01% octenidine at 20°C and 35°C was bactericidalto Borrelia afzeli; Borrelia garini, B. burgdorferi sensu stricto after 5 minutes treatment. Increase of the temperature to 35°C speed up the bactericidal effect to 1 minute. The bactericidal action of octenidine towards B. burgdorferi spirochetes growing in fibroblasts was less effective and needed a longer time to kill them than in the suspension.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

66

Numer

2

Opis fizyczny

p.259-263,ref.

Twórcy

  • Laboratory of Rickettsia, Chlamydia and Spirochetes, National Institute of Public Heath - National Institute of Hygiene, Warsaw, Poland
autor
  • Laboratory of Rickettsia, Chlamydia and Spirochetes, National Institute of Public Heath - National Institute of Hygiene, Warsaw, Poland
  • Laboratory of Rickettsia, Chlamydia and Spirochetes, National Institute of Public Heath - National Institute of Hygiene, Warsaw, Poland
  • Laboratory of Rickettsia, Chlamydia and Spirochetes, National Institute of Public Heath - National Institute of Hygiene, Warsaw, Poland

Bibliografia

  • Chmielewski T. and S. Tylewska-Wierzbanowska. 2010. Interactions between Borrelia burgdorferi and mouse fibroblasts. Pol. J. Microbiol. 59: 157–160.
  • Coburn J., G. Leong and G. Chaconas. 2013. Iluminating therole of the Borrelia burgdorferi adhesions. Trends Microbiol. 21: 372–379.
  • Embers M.E., S.W. Barthold, J.T. Borda, L. Bowers, L. Doyle,E. Hodzic, M.B. Jacobs, N.R. Hasenkampf, D.S. Martin, S. Nara-simhan and others. 2012. Persistence of Borrelia burgdorferi in rhesus macaques following antibiotic treatment of disseminated infection. PLoS One 7(1): e29914.
  • European Standard EN 1276: Chemical disinfectants and antiseptics – Quantitative suspension test for the evaluation of bactericidal activity of chemical disinfectants and antiseptics usedin food, industrial, domestic and institutional areas – Test method and requirements. European Committee for Standardization, Brussels 2009.
  • Groshong A.M. and J.S.Blevins. 2014. Insights into biology of Borrelia burgdorferi gained through the application of molecular genetics. Adv. Appl. Microbiol. 86: 41–143.
  • Harman M.W., S.M. Dunham-Ems, M.J. Caimano, A.A. Belper-ron, L.K. Bockenstedt, H.C. Fu, J.D. Radolf and C.W. Wolgemuth.2012. Heterogeneous motility of the Lyme disease spirochete in gelatin mimics dissemination throught tissue. Proc. Natl. Acad. Sci. USA109: 3059–3064
  • Harrison M.A. and I.F. Rae. 1997. General techniques of cell cultures, pp. 50, 72–73. Cambridge University Press.
  • Hodzic E., S. Feng, K. Holden, K.J. Freet and S.W. Barthold. 2008. Persistence of Borrelia burgdorferi following antibiotic treatment in mice. Antimicrob. Agents Chemother. 52: 1728–1736.
  • Motaleb A., J. Liu and R.M. Wooten. 2015. Spirocheta motility and chemotaxis in the natural enzotic cycle and development of Lyme disease. Curr. Op. Microbiol. 28: 106–113.
  • Stanek G., G.P. Wormser, J. Gray and F. Strle. 2012. Lyme borreliosis. Lancet 4:461–473.
  • Strabinger R.K., B.A. Summers, Y.F. Chang and M.J.G. Appel.1997. Persistence of Borrelia burgdorferi in experimentally infected dogs after antibiotic treatment. J. Clin. Microbiol. 15: 111–116.
  • Theophilus P.A.S., M.J. Victoria, K.M. Socarras, K.R. Filush,K. Gupta, D.F. Luecke and E. Sapi. 2015. Effectiveness of Stevia rebaudiana whole leaf extract against the various morphological forms of Borrelia burgdorferiin vitro. Eur. J. Microbiol. Immunol.4: 268–280.
  • Tylewska-Wierzbanowska S. and T. Chmielewski. 1997. The isolation of Borrelia burgdorferi spirochetes from clinical material in cell line cultures. Zentralbl. Bakteriol. 286: 363–370.
  • Wolgemuth C.W. Flagellar motility of the pathogenic spirochetes. 2015. Semin. Cell Dev. Biol. 46: 104–112.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

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