Growth and metabolic responses of contrasting chickpea (Cicer arietinum L.) genotypes to chilling stress at reproductive phase

• Autorzy: Kumar S. , Malik J. , Thakur P. , Kaistha S. , Sharma K. D. , Upadhyaya H. D. , Berger J. D. , Nayyar H.
• Języki publikacji: EN

Abstrakty

EN

Chilling stress (<10°C) at reproductive phase of chickpea results in abortion of flowers and pods leading to poor yield. The metabolic causes associated with cold sensitivity of chickpea are not well understood. Hence, in the present study, we evaluated four chickpea genotypes (ICC 16348, ICC 16349, PBG1 and GPF2) having contrasting cold sensitivity for their reproductive growth and metabolism subjected to cold stress (average day temperature: 17.6°C; average night temperature: 4.9°C). Genotypes ICC 16348 and ICC 16349 showed flowering and set pods, while PBG1 and GPF2 failed to do so during the stress conditions indicating the former to be cold tolerant. The stress injury in the leaves such as increase in electrolyte leakage, decrease in chlorophyll content and relative leaf water content was significantly less in ICC 16348 and ICC 16349 genotypes. The analysis of carbohydrates indicated total sugars and starch to be present in greater content in ICC 16348 and ICC 16349 relative to PBG1 and GPF2 genotypes. The enzymes related to carbohydrate metabolism such as β-amylase, invertase and sucrose synthase showed significantly higher activity in the leaves of ICC 16348 and ICC 16349 compared to the other two genotypes. PBG1 and GPF2 genotypes experienced greater oxidative stress measured as malondialdehyde and hydrogen peroxide. ICCV 16348 and ICC 16349 possessed significantly higher levels of enzymatic (superoxide dismutase, catalase, ascorbate peroxidase) and non-enzymatic antioxidants (proline and ascorbic acid) relative to PBG1 and GPF2. Particularly, proline and ascorbic acid were markedly higher in cold-tolerant genotypes compared to the sensitive ones suggesting their deciding role in governing the cold tolerance.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2011
• Tom: 33
• Numer: 3
• Opis fizyczny: p.779-787,fig.,ref.

Twórcy

autor

Kumar S.

• Department of Botany, Panjab University, Chandigarh, India

autor

Malik J.

• Department of Botany, Panjab University, Chandigarh, India

autor

Thakur P.

• Department of Botany, Panjab University, Chandigarh, India

autor

Kaistha S.

• Department of Botany, Panjab University, Chandigarh, India

autor

Sharma K. D.

• Biotechnology Centre, H.P.K.V., Palampur, India

autor

Upadhyaya H. D.

• ICRISAT, Hyderabad, India

autor

Berger J. D.

• CSIRO Plant Industry, Perth, Australia

autor

Nayyar H.

• Department of Botany, Panjab University, Chandigarh, India

Bibliografia

• Arnon DI (1949) Copper enzyme in isolated chloroplasts: polyphenol oxidase in Beta vulgaris. Plant Physiol 24:1–15
• Aroca R, Tognoni F, Irigoyen JJ, Sánchez-Díaz M, Pardossi A (2001) Different root low temperature response of two maize genotypes differing in chilling sensitivity. Plant Physiol Biochem 39:1067–1073
• Barrs HD, Weatherley PE (1962) A re-examination of the relative turgidity techniques for estimating water deficits in leaves. Aust J Biol Sci 15:413–428
• Bates LS, Woldren RP, Teare ID (1973) Rapid determination of free proline for water stress studies. Plant Soil 39:205–208
• Bertin P, Bouharmont J, Kinet JM (1997) Somaclonal variation and improvement of chilling tolerance in rice: changes in chillinginduced chlorophyll fluorescence. Crop Sci 37:1727–1735
• Bowler C, Montagu MV, InzeD(1992) Superoxide dismutase and stress tolerance. Annu Rev Plant Physiol Plant Mol Biol 43:83–116
• Capell B, Do¨rffling K (1993) Genotype-specific differences in chilling tolerance of maize in relation to chilling-induced changes in water status and abscisic acid accumulation. Physiol Plant 88:638–646
• Chen WP, Li PH (2002) Membrane stabilization by abscisic acid under cold aids proline in alleviating chilling injury in maize (Zea mays L.) cultured cells. Plant Cell Environ 25:955–962
• Clarke HJ, Siddique KHM (2004) Response of chickpea genotypes to low temperature stress during reproductive development. Field Crops Res 90:323–334
• Dhindsa RS, Dhindsa PP, Thorpe TA (1981) Leaf senescence: correlated with increased level of membrane permeability and lipid peroxidation, and decreased levels of superoxide dismutase and catalase. J Exp Bot 32:93–101
• Du YC, Nose A (2002) Effects of chilling temperature on the activity of enzymes of sucrose synthesis and the accumulation of saccharides in leaves of three sugarcane cultivars differing in cold sensitivity. Photosynthetica 40:389–395
• Duncan DR, Widholm JM (1987) Proline accumulation and its implication in cold tolerance of regenerable maize callus. Plant Physiol 83:703–708
• Guo Z, Ou W, Lu S, Zhong Q (2006) Differential responses of antioxidative system to chilling and drought in four rice cultivars differing in sensitivity. Plant Physiol Biochem 44:828–836
• Hare PD, Cress WA (1997) Metabolic implications of stress-induced proline accumulation in plants. Plant Grow Regul 21:79–102
• Hawker JS, Hatch MD (1965) Mechanism of sugar storage by mature stem tissue of sugarcane. Physiol Plant 18:444–453
• Hawker JS, Walker RR, Ruffner HP (1976) Invertase and sucrose synthase in flowers. Phytochemistry 15:1411–1443
• Heath RL, Packer L (1968) Photoperoxidation in isolated chloroplast. I. Kinetics and stoichiometry of fatty acid peroxidation. Arch Biochem Biophys 125:189–198
• Huang M, Guo Z (2005) Responses of antioxidative system to chilling stress in two rice cultivars differing in sensitivity. Biol Plant 49:81–84
• Ismail MA, Ella ES, Vergara GV, Mackill DJ (2009) Mechanisms associated with tolerance to flooding during germination and early seedling growth in rice (Oryza sativa). Ann Bot 103:197–209
• Janda T, Kósa EI, Szalai G, Páldi E (2005) Investigation of antioxidant activity in maize during low temperature stress. Acta Biol Szegediensis I49:53–54
• Janowiak F, Dorffling K (1996) Chilling tolerance of 10 maize genotypes as related to chilling-induced changes in ACC and MACC contents. J Agron Crop Sci 177:175–184
• Janowiak F, Markowski A (2008) Changes in leaf water relations and injuries in maize seedlings induced by different chilling conditions. J Agron Crop Sci 172:19–28
• Kaur S, Gupta AK, Kaur N, Sandhu JS, Gupta SK (2009) Antioxidative enzymes and sucrose synthase contribute to cold stress tolerance in chickpea. J Agron Crop Sci 195:393–397
• Kocsy G, Brunner M, Rüegsegger A, Stamp P, Brunold C (1996) Glutathione synthesis in maize genotypes with different sensitivity to chilling. Planta 198:365–370
• Kuk YI, Shin JS, Burgos NR, Hwang TE, Han O, Cho BH, Jung S, Guh JO (2003) Antioxidative enzymes offer protection from chilling damage in rice plants. Crop Sci 43:2109–2117
• McReedy RM, Guggolz J, Silviera V, Owens HS (1950) Determination of starch and amylose in vegetables. Anal Chem 22:1156
• Mitter R (2002) Oxidative stress, antioxidants and stress tolerance. Trends Plant Sci 7:405–410
• Morsy MR, Jouve L, Hausman JF, Hoffmann L, Stewart JM (2007) Alteration of oxidative and carbohydrate metabolism under abiotic stress in two rice (Oryza sativa L.) genotypes contrasting in chilling tolerance. J Plant Physiol 164:157–167
• Mukherjee SP, Choudhuri MA (1983) Implications of water stress induced changes in the levels of endogenous ascorbic acid and hydrogen peroxide in Vigna seedlings. Physiol Plant 58:166–170
• Nakano Y, Asada K (1981) Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant Cell Physiol 22:867–880
• Nayyar H, Gupta D (2006) Differential sensitivity of C₃ and C₄ plants to water deficit stress: association with oxidative stress and antioxidants. Environ Exp Bot 58:106–113
• Nayyar H, Bains T, Kumar S (2005) Low temperature induced floral abortion in chickpea: relationship to abscisic acid and cryoprotectants in reproductive organs. Environ Exp Bot 53:39–47
• Nygaard P (1977) Utilization of exogenous carbohydrates for the growth and starch synthesis in pine pollen suspension cultures. Physiol Plant 39:206–210
• Patton AJ, Cunningham SM, Volenec JJ, Reicher ZJ (2007) Differences in freeze tolerance of Zoysia grasses. II. Carbohydrate and proline accumulation. Crop Sci 47:2170–2181
• Premchandra GS, Sameoka H, Ogata S (1990) Cell osmotic membrane-stability, an indication of drought tolerance, as affected by applied nitrogen in soil. J Agric Res 115:63–66
• Sairam RK, Saxena DC (2000) Oxidative stress and antioxidants in wheat genotypes: possible mechanism of water stress tolerance. J Agron Crop Sci 184:55–61
• Santoiani CS, Tognetti JA, Pontis HG, Salerno GL (2006) Sucrose and fructan metabolism in wheat roots at chilling temperatures. Physiol Plant 87:84–88
• Saruyama H, Tanida M (1995) Effect of chilling on activated oxygenscavenging enzymes in low temperature-sensitive and -tolerant cultivars. Plant Sci 109:105–113
• Shalata A, Neumann PM (2001) Exogenous ascorbic acid (vitamin C) increases resistance to salt stress and reduces lipid peroxidation. J Exp Bot 52:2207–2211
• Shen W, Nada K, Tachibana S (1999) Effect of chilling treatment on enzymic and non-enzymic antioxidant activities in leaves of chilling tolerant and chilling sensitive cucumber (Cucumis sativus L.). J Jpn Soc Hort Sci 68:967–973
• Shuster L, Gifford RH (1962) Assay of amylases. Arch Biochem Biophys 194:534–540
• Smirnoff N (1995) Antioxidant systems and plant response to the environment. In: Smirnoff N (ed) Environment and plant metabolism: flexibility and acclimation. Bios Scientific Publishers, Oxford, UK, pp 217–243
• Smirnoff N (2000) Ascorbic acid: metabolism and functions of a multi-facetted molecule. Curr Opin Plant Biol 3:229–235
• Srinivasan A, Johansen C, Saxena NP (1998) Cold tolerance during early reproductive growth of chickpea (Cicer arietinum L.): characterization of stress and genetic variation in pod set. Field Crops Res 57:179–191
• Sumner JB (1935) A more specific reagent for determination of sugar in urine. J Biol Chem 69:393–396
• Teranishi Y, Tanaka A, Osumi M, Fukui S (1974) Catalase activities of hydrocarbon-utilizing Candida yeasts. Agric Biol Chem 38:1213–1220
• Thiaw S, Hall AE (2004) Comparison of selection for either leaf electrolyte-leakage or pod set in enhancing heat tolerance and grain yield of cowpea. Field Crops Res 86:239–253
• Wang WB, Kim YH, Lee HS, Deng XP, Kwak SS (2009) Differential antioxidation activities in two alfalfa cultivars under chilling stress. Plant Biotech Rep 3:301–307
• Yemm EW, Willis AJ (1954) The estimation of carbohydrates in plant extracts by anthrone. Biochem J 57:508–514
• Ying J, Lee EA, Tollenaar M (2000) Response of maize leaf photosynthesis to low temperature during the grain-filling period. Field Crops Res 68:87–96
• Yu JQ, Zhou YH, Huang LF, Allen DJ (2002) Chill-induced inhibition of photosynthesis: genotypic variation within Cucumis sativus. Plant Cell Physiol 43:1182–1188
• Zhang Y, Dai JY, Su ZS, Xu SC, Cheng J (1995) The injurious effect of low temperature at booting stage on female inflorescence of maize. Acta Agron Sin 21:235–239

Uwagi

rekord w opracowaniu