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2010 | 28 | 3 |

Tytuł artykułu

Effect of exogenous glutathione in extender on the freezability of Nili-Ravi buffalo (Bubalus bubalis) bull spermatozoa

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
The study was designed to investigate the effect of exogenous glutathione supplementation (0.0,0.5, 1.0, 2.0 and 3.0 mM) in tris-citric acid extender on post-thaw sperm motility, viability, plasma membrane and acrosomal integrity of buffalo (Bubalus bubalis) spermatozoa at 0, 3 and 6 hours after thawing. Glutathione supplementation of the extender upto 2.0 mM concentration increased (P≤0.05) sperm motility, viability, plasma membrane integrity and acrosomal integrity at 0, 3 and 6 hours after thawing in a dose-dependent manner compared to the control. However, glutathione supplementation at higher concentration (3.0 mM) was not beneficial for any of semen quality parameters. It is concluded that glutathione supplementation (up to 2.0 mM) of the extender improves the post-thaw quality of buffalo bull spermatozoa.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

28

Numer

3

Opis fizyczny

p.235-244,fig.,ref.

Twórcy

autor
  • Animal Physiology Laboratory, Department of Zoology, Pir Mehr Ali Shah Arid Agriculture University of Rawalpindi, 46300, Pakistan
autor
  • Animal Physiology Laboratory, Department of Zoology, Pir Mehr Ali Shah Arid Agriculture University of Rawalpindi, 46300, Pakistan
autor
  • Animal Physiology Laboratory, Department of Zoology, Pir Mehr Ali Shah Arid Agriculture University of Rawalpindi, 46300, Pakistan
  • Animal Physiology Laboratory, Department of Zoology, Pir Mehr Ali Shah Arid Agriculture University of Rawalpindi, 46300, Pakistan
autor
  • Animal Physiology Laboratory, Department of Zoology, Pir Mehr Ali Shah Arid Agriculture University of Rawalpindi, 46300, Pakistan
autor
  • Department of Veterinary Clinical Sciences, The Royal Veterinary College, Hawkshead Lane, North Mymms Hatfield, Hertfordshire AL9 7TA, U.K.
autor
  • Animal Physiology Laboratory, Department of Zoology, Pir Mehr Ali Shah Arid Agriculture University of Rawalpindi, 46300, Pakistan

Bibliografia

  • AITKEN R.J., GORDON E., HARKISS D., TWIGG J.P., MILNE P., JENNING Z., IRVINE, D.S.,1998 – Relative impact of oxidative stress on the functional competence and genomic integrity of human spermatozoa. Biology of Reproduction 59, 1037-1046.
  • AKHTER S., ANSARI M.S., ANDRABI S.M.H., ULLAH N., QAYYUM M., 2008 – Effect of antibiotics in extender on bacterial control and spermatozoal quality of cooled buffalo (Bubalus bubalis) bull semen. Reproduction in Domestic Animals 10, 1439-0531.
  • AKIYAMA M., 1999 – In vivo scavenging effect of ethylcysteine on reactive oxygen species in human semen. Nippon Hinyokika Gakkai Zasshi 90, 421–428.
  • ALVAREZ J.G., STOREY B.T., 1983 – Role of superoxide dismutase in protecting rabbit spermatozoa from O2 toxicity due to lipid peroxidation. Biology of Reproduction 28, 183-197.
  • ANDRABI S.M.H., ANSARI M.S., ULLAH N., ANWAR M., MEHMOOD A., AKHTER S.,2008 – Duck egg yolk in extender improves the freezability of buffalo bull spermatozoa. Animal Reproduction Science 104, 427-433.
  • BAILEY J.L., BILODEAU J.F., CORMIER N., 2000 – Semen cryopreservation in domestic animals:A damaging and capacitating phenomena. Journal of Andrology 22, 508-515.
  • BAUMBER J., BALL B.A., LINFOR J.J., 2005 – Assessment of the cryopreservation of equine spermatozoa in the presence of enzyme scavengers and antioxidants. American Journal of Veterinary Research 66, 772-779.
  • BECONI M.T., FRANCIA C.R., MORA N.G., AFFRANCHINO M.A., 1993 – Effect of natural antioxidants on frozen bovine semen preservation. Theriogenology 40, 841-851.
  • BILODEAU J.F., BLANCHETTE S., GAGNON C., SIRAD M.A., 2000 – Levels of antioxidant defenses are decreased in bovine spermatozoa after a cycle of freezing and thawing. Molecular Reproduction and Development 55, 282-288.
  • BILODEAU J.F., BLANCHETTE S., GAGNON C., SIRAD M.A., 2001 – Thiols prevent H2O2 mediated loss of sperm motility in cryopreserved bull semen. Theriogenology 56, 275-286.
  • BRITO L.F.B., BARTH A.D., BILODEAU-GOESEEL S., PANICH P.L., KASTELIC J.P., 2003 – Comparison of methods to evaluate the plasmalemma of bovine sperm and their relationship with in vitro fertilization rate. Theriogenology 60, 1539–1551.
  • BUCAK M.N., TEKIN N., 2007 – Protective effect of taurine, glutathione and trehalose on the liquid storage of ram semen. Small Ruminant Research 73, 103-108.
  • CHATTERJEE S., LAMIRANDE E.D., GAGNON C., 2001 – Cryopreservation alters membrane sulfhydryl status of bull spermatozoa: Protection of oxidized glutathione. Molecular Reproduction and Development 60, 498-506.
  • COTRAN R.S., KUMAR V., ROBINS S.L., 1989 – Pathologic basis of diseases, 4th edn, WB Saunders Co. Philadelphia, 9-16.
  • GADEA J., GUMBAO D., NOVAS S.C., ZQUEZ F.A.Z., GRULLO L.A., GARDO G.C., 2007 – Supplementation of the dilution medium after thawing with reduced glutathione improves function and the in vitro fertilizing ability of frozen-thawed bull spermatozoa. Andrology 7, 1-10.
  • GADEA J., SELLES E., MARCO M.A., COPY P., MATAS C., ROMAR R., RUIZ S., 2004 –Decrease in glutathione content in boar sperm cryopreservation. Effect of the addition of reduced glutathione to the freezing and thawing extenders. Theriogenology 62, 690-701.
  • JEYENDRAN R.S., VAN DER VEN H.H., PEREZ-PELAEZ M., CRABO B.G., ZANEVELD L.J.D., 1984 – Development of an assay to assess the functional integrity of the human sperm membrane and its relationship to other semen characteristics. Journal Reproduction and Fertility 70, 219-228.
  • KANKOFER M., KOLM G., AURICH J., 2005 – Activity of glutathione peroxidase, superoxide dismutase and catalase and lipid peroxidation intensity in stallion semen during storage at 5 oC.Theriogenology 63, 1354-1365.
  • LENZI A., GANDINI L., LOMABARDO F., PICARDO M., MARESCA V., PANFILI E., 2002 –Polyunsaturated fatty acids of germ cell membranes, glutathione and glutathione-dependant enzyme-PHGPx from basic to clinic. Contraception 65, 301-304.
  • MEISTER A., ANDERSSSON M.E., 1983 – Glutathione. Annual Review of Biochemistry 52, 711-760.
  • MUNSI M.N., BHUIYAN M.M.U., MAJUMDER S., ALAM M.G.S., 2007 – Effects of exogenous glutathione on the quality of chilled bull semen. Reproduction in Domestic Animals 42, 358–362.
  • NICHI M., BOLS P.E.J., ZU¨GE R.M., BARNABE V.M., GOOVAERTS I.G.F., BARNABE R.C.,CORTADA C.N.M., 2006 – Seasonal variation in semen quality in Bos indicus and Bos taurus bulls raised under tropical conditions. Theriogenology 66, 822-828.
  • SILVA P.F.N., GADELLA B.M., 2006 – Detection of damage in mammalian sperm cells.Theriogenology 65, 958-978.
  • SINHA M.P., SINHA A.K., SINGH B.K., PRASAD P.L., 1996 – The effect of glutathione on the motility, enzyme leakage and fertility of frozen goat semen. Theriogenology 41, 237–243.
  • SREEJITH J. N., BRAR A.S., AHUJA C.S., SANGHA S.P.S., CHAUDHARY K.C., 2006 – A comparative study on lipid peroxidation, activities of antioxidant enzymes and viability of cattle and buffalo bull spermatozoa during storage at refrigeration temperature. Animal Reproduction Science 96, 21-29.
  • STRADAIOLI G., NORO T., SYLLA L., MONACI M., 2007 – Decrease in glutathione (GSH) content in bovine sperm after cryopreservation: Comparison between two extenders. Theriogenology 67, 1249-1255.
  • URATA K., NARAHARA H., TANAKAY E., GASHIRU T., TAKAYAMA E., MIYAKAW I., 2001 – Effect of endotoxin-induced reactive oxygen species on sperm motility. Fertility and Sterility 76,163-166.
  • VISHWANATH R., SHANNON P., 1997 – Do sperm cells age? A review of the physiological changes in sperm duing storage at ambient temperature. Reproduction, Fertility and Development 9,321-332.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

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