In vitro-regenerated weltand sedge Eriophorum vaginatum L. is genetically stable

• Autorzy: Rewers M. , Kisiala A. , Drouin J. , Sliwinska E. , Cholewa E.
• Języki publikacji: EN

Abstrakty

EN

Eriophorum vaginatum L. is a promising species for phytostabilization, restoration, or creation of wetlands, because it can survive in cold, nutrient-poor, or metal-contaminated soils. However, its propagation on a large scale is problematic due to the infrequent production of viable seeds, seed dormancy, and the limitations of reproduction by rhizomes. A technique to rapidly and effectively produce large quantities of outplanting stock of this species was sought. Seeds of E. vaginatum were cultured on Murashige and Skoog (MS) medium supplemented with plant growth regulators at different concentrations. The highest regeneration rate was obtained on MS medium supplemented with 2.26 µM 2,4-dichlorophenoxyacetic acid (2,4-D) and 2.32 µM kinetin (KIN) for callus induction, and 17.76 µM BA (6-benzylaminopurine) for shoot regeneration as well as when 2.26 µM 2,4-D and 4.65 µM KIN was added to the callus-induction medium, and 8.88 µM or 17.76 µM BA to the shootregeneration medium. The regenerated shoots were rooted on MS medium without growth regulators and acclimatized in a greenhouse. Genetic stability of the in vitro regenerants was determined using flow cytometry and random amplified polymorphic DNA. Cytometric analysis revealed that the nuclear DNA content was similar in all plant materials and amounted to about 0.8 pg/2C. The PCR amplification products were monomorphic in callusderived plants and similar to plants grown in a field. Lack of genome size variation and polymorphism within the regenerants indicates that the detailed E. vaginatum micropropagation protocol allows the production of a large number of genetically stable plants.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2012
• Tom: 34
• Numer: 6
• Opis fizyczny: p.2197-2206,fig.,ref.

Twórcy

autor

Rewers M.

• Department of Biology, Nipissing University, 100 College Drive, North Bay, ON P1B 8L7, Canada
• Laboratory of Molecular Biology and Cytometry, Department of Plant Genetics and Biotechnology, University of Technology and Life Sciences, Kaliskiego Ave. 7, 85-789 Bydgoszcz, Poland

autor

Kisiala A.

• Department of Biology, Nipissing University, 100 College Drive, North Bay, ON P1B 8L7, Canada
• Laboratory of Molecular Biology and Cytometry, Department of Plant Genetics and Biotechnology, University of Technology and Life Sciences, Kaliskiego Ave. 7, 85-789 Bydgoszcz, Poland

autor

Drouin J.

• Department of Biology, Nipissing University, 100 College Drive, North Bay, ON P1B 8L7, Canada
• Laboratory of Molecular Biology and Cytometry, Department of Plant Genetics and Biotechnology, University of Technology and Life Sciences, Kaliskiego Ave. 7, 85-789 Bydgoszcz, Poland

autor

Sliwinska E.

• Laboratory of Molecular Biology and Cytometry, Department of Plant Genetics and Biotechnology, University of Technology and Life Sciences, Kaliskiego Ave. 7, 85-789 Bydgoszcz, Poland

autor

Cholewa E.

• Department of Biology, Nipissing University, 100 College Drive, North Bay, ON P1B 8L7, Canada

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