Proteomic discovery of matrix metalloproteinase-9 substrates

• Autorzy: Bajor M. , Michaluk P. , Kaczmarek L.
• Języki publikacji: EN

Abstrakty

EN

Elucidation of protease substrates (“proteodegradomes”) is essential for understanding the proteolytic pathways and their networks and thus their role in the regulation of cell function. Matrix metalloproteinase-9 (MMP-9) is expressed by the adult brain and released in response to enhanced neuronal activity. It is well established that MMP-9 is involved in neuronal plasticity, including long-term potentiation, learning and memory. Under pathological conditions, during excitotoxicity, stroke and traumatic brain injury, MMP-9 is detrimental to the brain tissue, probably because of its enhanced activity. MMP-9 is locally inhibited by endogenous tissue inhibitors of metalloproteinase 1 (TIMP-1). In the current studies we optimized the isolation of synaptoneurosomal fractions from the murine hippocampus. In order to induce MMP-9 activity the synaptoneurosomal fractions were treated with 50 μM of glutamate for 20 min. To identify MMP-9 substrates, we compared the synaptic fractions isolated from wild type and MMP-9 knockout mice by two-dimensional electrophoresis (2-DE). We have found the differences in the 2-D gel patterns. Further studies will be complemented by in-gel digestion of the protein spots of interest, mass spectrometry of the resultant peptides, and peptide mass fi ngerprinting to identify each protein.

• Wydawca: -
• Czasopismo: Acta Neurobiologiae Experimentalis
• Rocznik: 2009
• Tom: 69
• Numer: 3
• Opis fizyczny: p.335

Twórcy

autor

Bajor M.

• Department of Molecular and Cellular Neurobiology, Nencki Institute of Experimental Biology Polish Academy of Sciences, Warsaw, Poland

autor

Michaluk P.

• Department of Molecular and Cellular Neurobiology, Nencki Institute of Experimental Biology Polish Academy of Sciences, Warsaw, Poland

autor

Kaczmarek L.

• Department of Molecular and Cellular Neurobiology, Nencki Institute of Experimental Biology Polish Academy of Sciences, Warsaw, Poland