Histomorphological and morphometric studies of the pancreatic islet cells of diabetic rats treated with extracts of Annona muricata

• Autorzy: Adeyemi D.O. , Komolafe O. A. , Adewole O. S. , Obuotor E. M. , Abiodun A. A. , Adenowo T. K.
• Języki publikacji: EN

Abstrakty

EN

Microanatomical changes in the pancreatic islet cells of streptozotocin induced diabetic Wistar rats were studied after treatment with methanolic extracts of Annona muricata leaves. Thirty adult Wistar rats were randomly assigned into three groups (control, untreated diabetic group, and A. muricata-treated diabetic group) of ten rats each. Diabetes mellitus was experimentally induced in groups B and C by a single intra-peritoneal injection of 80 mg/kg streptozotocin dissolved in 0.1 M citrate buffer. The control rats were intraperitoneally injected with an equivalent volume of citrate buffer. Daily intra peritoneal injections of 100 mg/kg A. muricata were administered to group C rats for two weeks. Post sacrifice the pancreases of the rats were excised and fixed in Bouin’s fluid. The tissues were processed for paraffin embedding and sections of 5 µm thickness were produced and stained with H & E, Gomori aldehyde fuchsin, and chrome alum haematoxylin-phloxine for demonstration of the β-cells of islets of pancreatic islets. Histomorphological and morphometric examination of the stained pancreatic sections showed a significant increase in the number, diameter, and volume of the β-cells of pancreatic islets of the A. muricata-treated group (5.67 ± 0.184 N/1000 µm², 5.38 ± 0.093 µm and 85.12 ± 4.24 µm³, respectively) when compared to that of the untreated diabetic group of rats (2.85 ± 0.361 N/1000 µm², 2.85 ± 0.362 µm and 69.56 ± 5.216 µm³, respectively). The results revealed regeneration of the β-cells of islets of pancreatic islet of rats treated with extract of A. muricata. (Folia Morphol 2010; 69, 2: 92–100)

Słowa kluczowe

EN

histomorphology; morphometry; pancreatic cell; islet cell; diabetic rat; plant extract; Annona muricata; diabetes mellitus; streptozotocin; pancreas

• Wydawca: -
• Czasopismo: Folia Morphologica
• Rocznik: 2010
• Tom: 69
• Numer: 2
• Opis fizyczny: p.92-100,fig.,ref.

Twórcy

autor

Adeyemi D.O.

• Department of Anatomy and Cell Biology, Obafemi Awolowo University, Ile-Ife, Nigeria
• Department of Anatomy and Cell Biology, Faculty of Basic Medical Sciences, Obafemi Awolowo University, Ile-Ife, Nigeria

autor

Komolafe O. A.

• Department of Anatomy and Cell Biology, Obafemi Awolowo University, Ile-Ife, Nigeria

autor

Adewole O. S.

• Department of Anatomy and Cell Biology, Obafemi Awolowo University, Ile-Ife, Nigeria

autor

Obuotor E. M.

• Department of Biochemistry, Obafemi Awolowo University, Ile-Ife, Nigeria

autor

Abiodun A. A.

• Department of Anatomy and Cell Biology, Obafemi Awolowo University, Ile-Ife, Nigeria

autor

Adenowo T. K.

• Department of Anatomy, Olabisi Onabanjo University, Ikenne, Nigeria

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