Changes in antibiotic susceptibility pattern of atypical Staphylococcus aureus strains isolated from cows of the same herd in 2008-2010

• Autorzy: Malkinska-Horodyska M. , Kubiak J. , Lassa H. , Malinowski E.
• Języki publikacji: EN

Abstrakty

EN

The isolates of Staphylococcus aureus strains were examined phenotypically by cultural features, tube coagulase test and clumping factor (CF), and genotypically by conventional PCR. The strains had positive reaction in CF test, but were negative in tube coagulase test. The analysed strains from the same cows in each year expressed also nuc and coa genes. About 25% of the strains were examined by the disc diffusion method for their sensitivity to antibiotics. During three years, the strains were highly susceptible in vitro to amoxicillin with clavulanic acid, oxacillin, bacitracin, and cefoperazone (more than 90%), and highly resistant to tetracycline, neomycin, and streptomycin. Forty randomly chosen strains, and eight strains from the same cows in each year were analy sed for minimal inhibitory concentration of penicillin G using microdilution method. An increasing resistance to the penicillin was noted. Moreover, eight strains, the same in each year, were also examined for ß-lactamase production and methicillin resistance. No ß-lactamase producers and no methicillin resistant strains were found using phenotypic and genotypie methods. In conclusion, it can be stated that antimicrobial susceptibility can change from one year to another.

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2012
• Tom: 56
• Numer: 2
• Opis fizyczny: p.139-143,fig.,ref.

Twórcy

autor

Malkinska-Horodyska M.

• Department of Pathophysiology of Reproduction and Mammary Gland, National Veterinary Research Institute, 85-090 Bydgoszcz, Poland

autor

Kubiak J.

autor

Lassa H.

autor

Malinowski E.

Bibliografia

• 1. Bannerman T.L.: Staphylococcus, Micrococcus, and other catalase-positive cocci that grow aerobically. Manual of clinical microbiology. ASM Press, Washington DC, 2003.
• 2. Cabral da Silva Santos O., Mdenezes Barros E., Vasconcelos Paida Brito M.A., do Carmo de Freire Bastos M., Netto dos Santos K.R., Giambiagi-deMarval M.: Identification of coagulase-negative staphylococci from bovine mastitis using RFLP-PCR of the groEL gene. Vet Microbiol 2008, 130, 134-140.
• 3. Clinical and Laboratory Standards Institute. Performance Standards for Antimicrobial Disk and Dilution Susceptibility Tests for Bacteria Isolated from Animals. Approved Standard M31-A3. CLSI, Wayne, USA, 2008.
• 4. Coelho S.M.O., Reinoso E., Pereira I.A., Soares L.C., Demo M., Bogni C., Souza M.M.S.: Virulence factors and antimicrobial resistance of Staphylococcus aureus isolated from bovine mastitis in Rio de Janeiro. Pesq Vet Bras 2009, 29, 369-374.
• 5. Fox L.K., Beser T.E., Jackson S.M.: Evaluation of coagulase-negative variant of Staphylococcus aureus as a cause of intramammary infections in a herd of dairy cattle. J Am Vet Med Assoc 1996; 209, 1143-1146.
• 6. Garbacz K., Goliński J.: Atypical Staphylococcus aureus strains defective in coagulase or clumping factor (CF). Post Microbiol 2006, 45, 39-43.
• 7. Garrison L.L., Schukken Y.H., Hilton B.: Antibiotic susceptibility patterns for various bacterial intramammary pathogens over 15 years: results and analysis of a database. Proceedings of National Mastitis Council Annual Meeting. Atlanta, USA, 2000, pp. 215- 216.
• 8. Gianneechini R.E., Concha C., Franklin A.: Antimicrobial susceptibility of udder pathogens isolated from dairy herds in the west littoral region of Uruguay. Acta Vet Scand 2002, 43, 31-41.
• 9. Godden S.M., Jansen J.T., Leslie K.E., Smart N.L., Kelton D.F.: The effect of sampling time and sample handling on the detection of Staphylococcus aureus in milk from quarters with subclinical mastitis. Can Vet J 2002, 43, 38-42.
• 10. Gooraninejad S., Ghobanpoor M., Salati A.P.: Antibiotic susceptibility of staphylococci isolated from subclinical mastitis. Pak J Bio Sci 2007, 10, 2781-2783.
• 11. Gruet P., Maincent P., Berthelot X., Kaltsatos V.: Bovine mastitis and intramammary drug delivery: review and perspective. Adv Drug Deliv Rev 2001, 50, 245-259.
• 12. Jánosi S., Baltay Z.: Correlations among the somatic cell count of individual bulk milk, result of the California mastitis test and bacteriological status of the udder in dairy cows. Acta Vet Hung 2004, 52, 173-183.
• 13. Kumar R., Yadav B.R., Singh R.S.: Antibiotic resistance and pathogenicity factors in Staphylococcus aureus isolated from mastitic Sahiwal cattle. J Biosci 2011, 36, 175-188.
• 14. Lee J.H.: Methicillin (oxacillin) - resistant Staphylococcus aureus strains isolated from major food animals and their potential transmission to humans. Appl Environ Microbiol 2003, 69, 6489-6494.
• 15. Lyon B.R., Skurray R.: Antimicrobial resistance of Staphylococcus aureus: genetic basis. Microbiol Rev 1987, 51, 88-134.
• 16. Malinowski E., Pilaszek J., Kłossowska A., Sobolewska S., Sobolewski J.: Changes in susceptibility to antibiotics in bacteria isolated from clinical manifestations of mastitis in cows in the years 1987-1996. Medycyna Wet 1997, 53, 722-725.
• 17. Malinowski E., Kłossowska A.: Diagnostyka zakażeń i zapaleń gruczołu mlekowego krów. Published by PIWet, Pulawy, Poland, 2002.
• 18. Malinowski E., Kłossowska A., Kaczmarowski M., Lassa H., Kuźma K.: Antimicrobial susceptibility of staphylococci isolated from affected with mastitis cows. Bull Vet Inst Pulawy 2002, 46, 289-294.
• 19. Malinowski E., Lassa H., Kłossowska A., Smulski S., Kaczmarowski M.: Atypical Staphylococcus aureus as an aetiological agent of mastitis in cows. Bull Vet Inst Pulawy 2009, 53, 383-387.
• 20. McDevitt D., Vaudaux P., Foster T.J.: Genetic evidence that bound coagulase of Staphylococcus aureus is not clumping factor. Infect Immun 1992, 60, 1514-1523.
• 21. McDevitt D., Francois P., Vaudaux P., Foster T.J.: Molecular characterization of the clumping factor (fibrinogen receptor) of Staphylococcus aureus. Mol Microbiol 1994, 11, 237-248.
• 22. Moon J.S., Lee A.R., Kang H.M., Lee E.S., Kim M.N., Paik Y.H., Park Y.H., Joo Y.S., Koo H.S.: Phenotypic and genetic antibiogram of methicillin-resistant staphylococci isolated from bovine mastitis in Korea. J Dairy Sci 2007, 90, 1176-1185.
• 23. Moroni P., Pisoni G., Antonini M., Villa R., Boettcher P., Carli S.: Short communication: antimicrobial drug susceptibility of Staphylococcus aureus from subclinical bovine mastitis in Italy. J Dairy Sci 2006, 89, 2973- 2976.
• 24. Oliveira A.P. de, Watts J.L., Salmon S.A., Aerestrup F.M.: Antimicrobial susceptibility of Staphylococcus aureus isolated from bovine mastitis in Europe and in the United States. J Dairy Sci 2000, 83, 855-862.
• 25. Oliver S.P., Lewis M.J., Gillespie B.E., Dowlen H.H., Jaenicke E.C., Roberts R.K.: Prepartum antibiotic treatment of heifers: milk production, milk quality and economic benefit. J Dairy Sci 2003, 86, 1187-1193.
• 26. Owens W.E., Ray C.H., Watts J.L., Yancey R.J.: Comparison of success of antibiotic therapy during lactation and results of antimicrobial susceptibility tests for bovine mastitis. J Dairy Sci 1997, 80, 313-317.
• 27. Russi N.B., Bantar C., Calvinho L.F.: Antimicrobial susceptibility of Staphylococcus aureus causing bovine mastitis in Argentine dairy herds. Rev Argentina Microbiol 2008, 40, 116-119.
• 28. Salmon S.A., Watts J.L., Aarestrup F.M., Pankey J.W., Yancey R.J.: Minimum inhibitory concentrations for selected antimicrobial agents against organisms isolated from the mammary glands of dairy heifers in New Zealand and Denmark. J Dairy Sci 1998, 81, 570-578.
• 29. Sol J., Sampimon O.C., Barkema H.W., Schukken Y.H.: Factors associated with cure after therapy of clinical mastitis caused by Staphylococcus aureus. J Dairy Sci 2000, 83, 278-284.
• 30. Sutra L., Poutrel B.: Virulence factors involved in the pathogenesis of bovine intramammary infections due to Staphylococcus aureus. J Med Microbiol 1994, 40, 79.
• 31. Turutoglu H., Ercelik S., Ozturk D.: Antibiotic resistance of Staphylococcus aureus and coagulase-negative staphylococci isolated from bovine mastitis. Bull Vet Inst Pulawy 2006, 50, 41-45.
• 32. Ünal N., Istanbulluoğlu E., Gür D., Ünal S., Bulun H.: Genetic variation and antibiotic resistance patterns of Staphylococcus aureus isolates from human and bovine. Rev Méd Vét 2009, 10, 463-468.
• 33. Unakal C.G., Kaliwal B.B.: Prevalence and antibiotic susceptibility of Staphylococcus aureus from bovine mastitis. Vet World 2010, 3, 65-67.
• 34. Watts J.R., Salmon J.A.: Activity of selected antimicrobial agents against strains of Staphylococcus aureus isolated from bovine intramammary infections that produce ß-lactamase. J Dairy Sci 1997, 80, 788-791.
• 35. Werckenthin C., Cardoso M., Martel J.L., Schwarz S.: Antimicrobial resistance in staphylococci from animals with particular reference to bovine Staphylococcus aureus, porcine Staphylococcus hyicus, and canine Staphylococcus intermedius. Vet Res 2001, 32, 341-362.