Low-dose oral immunization with lyophilized tissue of herbicide-resistant lettuce expressing hepatitis B surface antigen for prototype plant-derived vaccine tablet formulation

• Autorzy: Pniewski T. , Kapusta J. , Bociąg P. , Wojciechowicz J. , Kostrzak A. , Gdula M. , Fedorowicz-Stronska O. , Wojcik P. , Otta H. , Samardakiewicz S. , Wolko B. , Plucienniczak A.
• Języki publikacji: EN

Abstrakty

EN

Efficient immunization against hepatitis B virus (HBV) and other pathogens with plant-based oral vaccines requires appropriate plant expressors and the optimization of vaccine compositions and administration protocols. Previous immunization studies were mainly based on a combination of the injection of a small surface antigen of HBV (S-HBsAg) and the feeding with raw tissue containing the antigen, supplemented with an adjuvant, and coming from plants conferring resistance to kanamycin. The objective of this study was to develop a prototype oral vaccine formula suitable for human immunization. Herbicide-resistant lettuce was engineered, stably expressing through progeny generation micrograms of S-HBsAg per g of fresh weight and formed into virus-like particles (VLPs). Lyophilized tissue containing a relatively low, 100-ng VLP-assembled antigen dose, administered only orally to mice with a long, 60-day interval between prime and boost immunizations and without exogenous adjuvant, elicited mucosal and systemic humoral anti-HBs responses at the nominally protective level. Lyophilized tissue was converted into tablets, which preserved S-HBsAg content for at least one year of room temperature storage. The results of the study provide indications on immunization methodology using a durable, efficacious, and convenient plant-derived prototype oral vaccine against hepatitis B.

• Wydawca: -
• Czasopismo: Journal of Applied Genetics
• Rocznik: 2011
• Tom: 52
• Numer: 2
• Opis fizyczny: p.125-136,fig.,ref.

Twórcy

autor

Pniewski T.

• Institute of Plant Genetics, Polish Academy of Sciences, Strzeszynska 34, 60-479 Poznan, Poland

autor

Kapusta J.

• Institute of Plant Genetics, Polish Academy of Sciences, Strzeszynska 34, 60-479 Poznan, Poland

autor

Bociąg P.

• Institute of Plant Genetics, Polish Academy of Sciences, Strzeszynska 34, 60-479 Poznan, Poland

autor

Wojciechowicz J.

• DNA Research Centre Ltd., Rubiez 46, 61-612 Poznan, Poland

autor

Kostrzak A.

• Institute of Plant Genetics, Polish Academy of Sciences, Strzeszynska 34, 60-479 Poznan, Poland

autor

Gdula M.

• Institute of Plant Genetics, Polish Academy of Sciences, Strzeszynska 34, 60-479 Poznan, Poland

autor

Fedorowicz-Stronska O.

• Institute of Plant Genetics, Polish Academy of Sciences, Strzeszynska 34, 60-479 Poznan, Poland

autor

Wojcik P.

• Institute of Medicinal Plants, Karola Libelta 27, 61-707 Poznan, Poland

autor

Otta H.

• Institute of Medicinal Plants, Karola Libelta 27, 61-707 Poznan, Poland

autor

Samardakiewicz S.

• Faculty of Biology, Adam Mickiewicz University, Umultowska 89, 61-614 Poznan, Poland

autor

Wolko B.

• Institute of Plant Genetics, Polish Academy of Sciences, Strzeszynska 34, 60-479 Poznan, Poland

autor

Plucienniczak A.

• Institute of Biotechnology and Antibiotics, Staroscinska 5, 05-216 Warszawa, Poland

Uwagi

PL

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