Somatostatin receptor 2 expression map in the barrel cortex

• Autorzy: Grabowska A. , Kossut M.
• Języki publikacji: EN

Abstrakty

EN

INTRODUCTION: Somatostatin (SST) is present in central and peripheral nervous system and it is a neurotransmitter or neuromodulator which can affect excitability and neuronal responses. In general SST is thought to inhibit excitatory synaptic transmission. Importantly, SST colocalizes with a gamma-aminobutyric acid (GABA) in inhibitory interneurons of cerebral cortex and hippocampus, and is widely used as a marker of interneurons. SST acts through a family of G protein-coupled receptors – somatostatin receptors (SSTRs) types 1–5. The SST-SSTRs system seems to be important in the development of cognitive processes like learning and memory. SSTR2 has been shown to be involved in the perceptual processes like anxiety and stress-induced behavior. Elucidating of SSTR2 distribution can significantly improve the knowledge about function of SST in the primary somatosensory cortex. AIM(S): The aim of these studies is to reveal the characteristic expression pattern of SSTR2 distribution within the barrel cortex. METHOD(S): Immunocytochemistry and immunofluorescent techniques on floating sections from wild type (C57BL/6) mice males were performed according to standard protocols using primary SSTR2 polyclonal antibody and were followed by Nissl or DAPI staining. RESULTS: The SSTR2 scattered expression pattern was observed within the barrel cortex. There was a slight difference according to its distribution as less evident signals were observed in the hollows of the barrels and the stronger ones in the barrel walls. The puncta of SSTR2 were densely concentrated on a neural cell bodies and dendrites within the barrel cortex. CONCLUSIONS: It is likely that the distribution of SSTR2 and action of SST-positive neurons together with GABA synthesis can via a pathway of disinhibition facilitate the learning-dependent plastic change. FINANCIAL SUPPORT: The authors are supported by Polish National Science Centre grant no. 2015/17/B/ NZ4/02016 given to Małgorzata Kossut.

• Wydawca: -
• Czasopismo: Acta Neurobiologiae Experimentalis
• Rocznik: 2017
• Tom: 77
• Numer: Suppl.1
• Opis fizyczny: p.100

Twórcy

autor

Grabowska A.

• Laboratory of Neuroplasticity, Department of Molecular and Cellular Neurobiology, Nencki Institute of Experimental Biology Polish Academy of Scieces, Warsaw, Poland

autor

Kossut M.

• Laboratory of Neuroplasticity, Department of Molecular and Cellular Neurobiology, Nencki Institute of Experimental Biology Polish Academy of Scieces, Warsaw, Poland
• University of Social Sciences and Humanities, Warsaw, Poland