Manipulacje genowe wzmacniajace stres oksydacyjny i odpowiedź antyoksydacyjną roślin w różnych warunkach środowiska

• Autorzy: Baczek-Kwinta R.

Warianty tytułu

EN

Genetic manipulations enhancing oxidative stress and plants' antioxidative response in various environmental conditions

• Języki publikacji: PL

Abstrakty

PL

Niniejsza praca stanowi przegląd dotychczasowych badań nad poprawą odporności na stresy środowiskowe drogą manipulacji genowych obejmujących enzymatyczne i niskocząsteczkowe elementy systemu antyoksydacyjnego. Intensyfikacja aktywności przeciwutleniaczy znajduje zastosowanie w ochronie roślin przed skutkami stresów abiotycznych - chłodu, mrozu, suszy, herbicydów foto-dynamicznych i innych. Natomiast w celu wzmocnienia nadwrażliwości na patogen i wyzwolenia odporności systemicznej (SAR) tworzone są transformanty o wzmożonej zdolności do tzw. wybuchu oddechowego, czyli do nadprodukcji tlenu singletowego, H₂O₂ i rodników tlenowych. Konstruowanie roślin modyfikowanych genetycznie (GM) drogami powyżej opisanymi napotyka jednak liczne problemy, przede wszystkim są to nieoczekiwane i niepożądane reakcje systemu antyoksydantów wywołane zmianami w stechiometrii sygnałów metabolicznych. W wielu przypadkach trudna do uzyskania jest też odporność krzyżowa transformantów.

EN

This article will give the overview of so far performed studies of the enhancement of plants' resistance to environmental stresses via genetic manipulations of both enzymatic and low-molecular weight antioxidants. The improvement of antioxidative activity has been applied to protect plants from abiotic stresses such as chill, frost, drought, photodynamic herbicides etc. On the other hand, in order to enhance the hypersensitivity to pathogen and to release the systemic acquired resistance, transformants with elevated ability to continuously overproduce singlet oxygen, H₂O₂ and oxygen radicals have been constructed. Manipulations described above have many difficulties, however. The main problems are the changes in the stoichiometry of metabolic signals leading to unpredictable and undesirable responses of antioxidant system. The cross-resistance of transgenes also needs to be conferred.

• Wydawca: -
• Czasopismo: Zeszyty Problemowe Postępów Nauk Rolniczych
• Rocznik: 2002
• Tom: 481
• Numer: 1
• Opis fizyczny: s.39-47,bibliogr.

Twórcy

autor

Baczek-Kwinta R.

Bibliografia

• Aono M., Saji H., Sakamoto A., Tanaka K., Kondo N., Tanaka K. 1995. Paraquat tolerance of transgenic Nicotiana tabacum with enhanced activities of glutathione reductase and superoxide dismutase. Plant Cell Physiol. 36: 1687-1691.
• Arisi A-C.M., Mocquot B., Lagriffoul A., Mench M., Foyer C.H., Jouanin L. 2000. Responses to cadmium in leaves of transformed poplars overexpressing gamma-gluta-mylcysteine synthetase. Physiol. Plant. 109: 143-149.
• Barata R.M., Chaparro A., Chabregas S.M., Gonzalez R., Labate CA., Azevedo R.A., Sarath G., Lea PJ., Silva-Filho M.C. 2000. Targeting of the soybean leghemo-globin to tobacco chloroplasts: effect on aerobic mechanism in transgenic plants. Plant Science 155: 193-202.
• Brüggemann W., Beyel V., Brodka M., Poth H., Weil M., Stockhaus J. 1999. Antioxidants and antioxidative enzymes in wild-type and transgenic Lycopersicon genotypes of different chilling tolerance. Plant Science 140: 145-154.
• Chamnongpol S., Willekens H., Moeder W., Langebartels C., Sandermann H., Van Montagu M., Inze D., Van Camp W. 1998. Defense activation and enhanced pathogen tolerance induced by H₂O₂in transgenic tobacco. Proc. Natl. Acad. Sci. USA 95: 5818-5823.
• Doulis A.G., Debian N., Kingston-Smith A.H., Foyer CH.H. 1997. Differential localization of antioxidants in maize leaves. Plant Physiol. 114: 1031-1037.
• Foyer Ch.H., Lopez-Delgado H., Dat J.F., Scott I.M. 1997. Hydrogen peroxide and glutathione-associated mechanisms of acclimatoiy stress tolerance and signalling. Physiol. Plant. 100: 241-254.
• Goto F., Yoshihara T., Saiki H. 2000. Iron accumulation and enhanced growth in transgenic lettuce plants expressing the iron-binding protein ferritin. Theoret. and Applied Genetics 100: 658-664.
• Horváth G.V., Oberschall A., Deak M., Sass 1., Vass I., Barna B., Kiraly Z., Hideg E., Feher A., Dudits D. 1999. Transgenic strategy to improve stress resistance of crop plants. J. Plant Biotechnol. 1: 61-68.
• Jacks TJ., De Lucca A J., Rajasekaran K., Stromberg K.D., Van Pee K.-H. 2000. An tifungal and peroxidative activities of nonheme chloroperoxidase in relation to transgenic plant protection. J. Agric. Food Chem. 48: 4561-4564.
• Kingston-Smith J.H., Foyer Ch.H. 2000. Overexpression of Mn-superoxide dismutase in maize leaves leads to increased monodehydroascorbate reductase, dehydroasco-bate reductase and glutathione reductase activities. J. Exp. Bot. 51: 167-187.
• Király Z. 2000. New aspects of breeding crops for disease resistance: the role of antioxidants, w: Use of agriculturally important genes in biotechnology. G. Hrazdina (red.). IOS Press. Ohmsa, Amsterdam-Berlin-Oxford-Tokyo-Washington, DC (NATO Science Series).
• Matamoros M.A., Baird L.M., Escuredo P.R., Dalton D.A., Minchin F.R., Iturbe-Ormatexe I., Rubio M.C, Moran J.F., Gordon AJ., Becana M. 1999. Stress-induced legume root nodule senescence. Physiological, biochemical and structural alterations. Plant Physiol. 121: 97-111.
• May M.J., Leaver CJ. 1993. Oxidative stimulation of glutathione synthesis in Arabi-dopsis thaliana suspension cultures. Plant Physiol. 103: 621-627.
• McKersie B.D., Bowley S.R., Harjanto E., Leprince O. 1996. Water-deficit tolerance and field peiformance of transgenic alfalfa overrexpressing superoxide dismutase. Plant Physiol. Ill: 1177-1181.
• McKersie B.D., Bowley S.R., Jones K.S. 1999. Winter swvival of transgenic alfalfa overexpressing superoxide dismutase. Plant Physiol. 119: 839-847.
• McKersie B.D., Murnghan J., Jones K.S., Bowley S.R. 2000. Iron-superoxide dismutase expression in transgenic alfalfa increases winter survival without a detectable increase in photosynthetic oxidative stress tolerance. Plant Physiol. 122: 1427-1437.
• Miyagawa Y., Tamoi M., Shigeoka S. 2000. Evaluation of the defense system in chlo-roplasts to photooxidative stress caused by paraquat using transgenic tobacco plants expressing catalase from Escherichia coli. Plant Cell Physiol. 41: 311-320.
• Noctor G., Strohm M., Jouanin L., Kunert K-J., Foyer Ch., Rennenberg H. 1996. Synthesis of glutathione in leaves of transgenic poplar overexpression y-glutamy[cysteine synthetase. Plant Physiol. 112: 1071-1078.
• Oh S.K., Park Y.S., Yang M.S. 1999. Transgenic tobacco plants expressing a mutant VU-4 calmodulin have altered nicotinamide co-enzyme levels and hydrogen peroxide levels. J. Biochem. Molec. Biol. 32: 1-5.
• Pastori G., Mullineaux P., Foyer Ch. 2000. Post-transcriptional regulation prevents accumulation of glutathione reductase protein and activity in the bundle sheath cells of maize. Plant Physiol. 122: 667-675.
• Potter F.J., Wiskich J.T., Dry I.B. 2001. The production of an inducible antisense alternative oxidase (Aoxla) plant. Planta 212: 215-221.
• Prasad T.K., Anderson M.B., Martin B.A., Stewart C.R. 1994. Evidence for chilling-induced oxidative stress in maize and a regulatory role for hydrogen peroxide. The Plant Cell 6: 65-74.
• Rajasekaran K., Cry J.W., Jacks T.J., Stromberg K.D., Cleveland T.E. 2000. Inhibition of fungal growth in planta and in vitro by transgenic tobacco expressing a bacterial nonheme chloroperoxidase gene. Plant Cell Reports 19: 333-338.
• Roxas V.P., Lodhi S.A., Garrett D.K., Mahan J.R., Allen R.D. 2000. Stress tolerance in transgenic tobacco seedlings that overexpress glutathione-S-transferase/glutathione peroxidase. Plant Cell Physiol. 41: 1229-1234.
• Sen Gupta A., Heinen J.L., Holaday A.S., Burke J.J., Allen R.D. 1993. Increased resistance to oxidative stress in transgenic plants that overexpress chloroplastic Cu/Zn superoxide dismutase. Proc. Natl. Acad. Sci. USA 90: 1629-1633.
• Shen B., Jansen R.G., Bohnert HJ. 1997. Increased resistance to oxidative stress in transgenic plants by targeting mannitol biosynthesis to chloroplasts. Plant Physiol. 113: 1177-1183.
• Strohm M., Eiblmeier M., Langebartels Ch., Jouanin L., Polle A., Sandermann H., Rennenberg H. 1999. Responses of transgenic poplar (Populus tremula x P. alba) overexpressing glutathione synthetase or glutathione reductase to acute ozone stress visible injury and leaf gas exchange. J. Exp. Bot. 332: 365-374.
• Torsethaugen G., Pitcher L.H., Zilinskas B.A., Pell E.J. 1997. Overproduction of ascorbate peroxidase in the tobacco chloroplast does not provide protection against ozone. Plant Physiol. 114: 529-537.
• Van Breusegem E, Van Montagu M., Inze D. 1998. Engineering stress tolerance in maize. Outlook Agric. 27: 115-124.
• Van Breusegem E, Slooten L., Stassart J-M., Moens T., Botterman J., Van Montagu M., Inze D. 1999a. Overproduction of Arabidopsis thaliana FeSOD confers oxidative stress tolerance to transgenic maize. Plant Cell Physiol. 40: 515-523.
• Van Breusegem E, Slooten L., Stassart J-M., Moens T., Botterman J., Moens T., Van Montagu M., Inze D. 1999b. Effects of overproduction of tobacco MnSOD in maize chloroplasts on foliar tolerance to cold and oxidative stress. J. Exp. Bot. 50: 71-78.
• Van Camp W., Capiau K., Van Montagu M., Inze D., Slooten L. 1996. Enhancement of oxidative stress tolerance in transgenic tobacco plants overproducing Fe-superoxide dismutase in chloroplasts. Plant Physiol. 112: 1703-1714.
• Vanleberghe G.C., McIntosh L. 1997. Alternative oxidase: from gene to function. Annu. Rev. Plant Physiol. Plant Mol. Biol. 48: 703-734.
• Wingate V.P.M., Lawton M.A., Lamb CJ. 1988. Glutathione causes a massive and selective induction of plant defense genes. Plant Physiol. 31: 205-211.
• Wu G., Shortt B J., Lawrance E.B., Leon J., Fitzsimmons K.C., Levine E. B., Raskin I., Shah D.M. 1997. Activation of host defense mechanisms by elevated production of H₂O₂ in transgenic plants. Plant Physiol. 115: 427-435.
• Zhu Y.L., Pilon-Smith E.A.H., Jouanin L., Terry N. 1999. Overexpression of glutathione synthetase in indian mustard enhances cadmium accumulation and tolerance. Plant Physiol. 119: 73-79.