Effect of medicinal leeches’ antigens on the proliferative response of human blood mononuclear cells and cytokine production in vitro

• Autorzy: Frolov A. K. , Litvinenko R. A.
• Języki publikacji: EN

Abstrakty

EN

The purpose of research is to study the influence of plant mitogens and antigens of the water-salt extract from the bodies of Hirudo verbana, H. medicinalis, H. orientalis on the reaction of lymphocyte blast-transformation and the synthesis of pro-inflammatory cytokines (IL-1β, IL-8, TNF-α) in patients’ cell culture supernatants before and after hirudotherapy. Research methods: the reaction of lymphocyte blast-transformation; the determination of proinflammatory cytokines by ELISA. After hirudotherapy increased values of the reaction of lymphocyte blasttransformation have got bigger in all stimulated types of cell culture, but reactivity on medicinal leeches antigens proceeded to unproductive immunogenesis (apoptosis and necrosis). In supernatants of mitogen- and antigen-stimulated lymphocyte cultures induced high levels of pro-inflammatory cytokines after hirudotherapy have decreased adequately to apoptotic induction of lymphocytes by medicinal leeches’ antigens.

• Wydawca: -
• Czasopismo: Annals of Parasitology
• Rocznik: 2015
• Tom: 61
• Numer: 2
• Opis fizyczny: p.97-104,fig.,ref.

Twórcy

autor

Frolov A. K.

• Faculty of Biology, Zaporizhzhya National University, Zhukovskogo 66, 69600 Zaporizhzhya, Ukraine

autor

Litvinenko R. A.

• Faculty of Biology, Zaporizhzhya National University, Zhukovskogo 66, 69600 Zaporizhzhya, Ukraine

Bibliografia

• [1] Baskova I.P., Isahanjan G.S. 2004. Girudoterapija. Nauka i praktika [Hirudotherapy. Science and practice]. Моnоlit, Moscow (In Russian).
• [2] Kamenev O.Ju., Baranovskij A.Ju. 2006. Lechenie pijavkami: teorija i praktika girudoterapii: rukovodstvo dlja vrachej [Treatment by leeches: theory and practice hirudotherapy: a guide for physicians]. IG «Ves’», St. Petersburg (In Russian).
• [3] Abdullah S., Dar L.M., Rashid A., Tewari A. 2012. Hirudotherapy / Leech therapy: applications and indications in surgery. Archives of Clinical and Experimental Surgery 1: 172-180.
• [4] Abdualkader A.M., Ghawi A.M., Alaama M., Awang M., Merzouk A. 2013. Leech therapeutic applications. Indian Journal of Pharmaceutical Sciences 75: 127- 137.
• [5] Prakash A., Parikshit D., Arun Raj G.R., Rao Prasanna N., Shailaja U. 2013. A review on the role of jalaukavacharana (hirudotherapy) in the management of the venous ulcer. Universal Journal of Pharmacy 2: 38-43.
• [6] Koeppen D., Aurich M., Rampp T. 2014. Medicinal leech therapy in pain syndromes: a narrative review. Wiener Medizinische Wochenschrift 164: 95-102.
• [7] Sobczak N., Kantyka M. 2014. Hirudotherapy in veterinary medicine. Annals of Parasitology 60: 89-92.
• [8] Porshinsky B.S., Saha S., Grossman M.D., Beery II P.R., Stawicki S. 2011. Clinical uses of the medicinal leech: a practical review. Journal of Postgraduate Medicine 57: 65-71.
• [9] Zaidi S.M., Jameel S.S., Zaman F., Jilani S., Sultana A., Khan S.A. 2011. A systematic overview of the medicinal importance of sanguivorous leeches. Alternative Medicine Review 16: 59-65.
• [10] Okka B. 2013. Hirudotherapy from past to present. European Journal of Basic Medical Sciences 3: 61- 65.
• [11] Singh A.P. 2010. Medicinal leech therapy (hirudotherapy): a brief overview. Complementary Therapies in Clinical Practice 16: 213-215.
• [12] Frolov V.M., Garnik T.P., Peresadin N.A., Visotskiy А.А. 2008. Dynamics of the indexes of the macrophages phagocyte system of patients with a syndrome of psychoemotional burning out down at hirudotherapy. Ukrainian Medical Almanac (Lugansk) 11: 175-179 (In Russian).
• [13] Frolov A.K., Litvinenko R.A. 2013. Peripheral blood’s immune parameters of patients with chronic disease during hirudotherapy. Fundamental Research (Moscow) 10: 589-593 (In Russian). Available on-line at: http://www.rae.ru/fs/pdf/2013/10-3/32325.pdf
• [14] Frolov A.K., Priluckiy A.S., Kopeyka V.V., Fedotov E.R., Litvinenko R.A. 2010. To the mechanism of immunotropic action of hirudotherapy. Immunology and Allergology: Science and Practice (Kiev) 3-4: 32- 34 (In Russian).
• [15] Thomson A.W., Lotze M.T. (Eds.) 2003. The cytokine handbook. 4th ed., 2 vols. Academic Press, Amsterdam.
• [16] Merry H.E., Phelan P., Doaks M., Zhao M., Mulligan M.S. 2015. Functional roles of tumor necrosis factor-alpha and interleukin 1-beta in hypoxia and reoxygenation. The Annals of Thoracic Surgery 99: 1200-1205.
• [17] Bigildeev A.E., Zezina E.A., Shipounova I.N., Drize N.J. 2015. Interleukin-1 beta enhances human multipotent mesenchymal stromal cell proliferative potential and their ability to maintain hematopoietic precursor cells. Cytokine 71: 246-254.
• [18] Shahzad A., Knapp M., Lang I., Köhler G. 2010. Interleukin 8 (IL-8) – a universal biomarker? International Archives of Medicine 3: 11.
• [19] Andrade P., Visser-Vandewalle V., Hoffmann C., Steinbusch H.W.M., Daemen M.A., Hoogland G. 2011. Role of TNF-alpha during central sensitization in preclinical studies. Neurological Sciences 32: 757-771.
• [20] Rowland-Jones S.L., McMichael A.J. 2000. Lymphocytes: A Practical Approach. 2nd ed. Oxford University Press. Inc., New York.
• [21] Patent UA 80665/ 10.06.2013. Bulletin №11. Frolov O.K., Lytvynenko R.O., Kopiika V.V., Fedotov Ye.R. Method for producing antigens from medicinal leeches. (In Ukrainian). Available on-line at: http://uapatents.com/5-80665-sposib-otrimannyaantigeniv- iz-medichno-pyavki.html
• [22] Friemel H. (Hrsg.) 1984. Immunologische Arbeitsmethoden. VEB Gustav Fischer Verlag, Jena.
• [23] Theml H., Diem H., Haferlach T. 2004. Color atlas of hematology. Practical microscopic and clinical diagnosis. 2nd ed. Thieme Stuttgart, New York.
• [24] Dunn O.J., Clark V.A. 2009. Basic statistics: a primer for the biomedical sciences. 4th ed. John Wiley & Sons, New York.
• [25] Nazarenko H.I., Kyshkun A.A. 2000. Klinicheskaja ocenka rezul’tatov laboratornyh issledovanij [Clinical evaluation of laboratory results]. Medicine, Moscow (In Russian).
• [26] Sher A., Muller-Berat C.N., Ahmad S. 1981. Transformation of hemopoietic stem cells by phytohemagglutinin (PHA) II. Mechanism of action. Experimental Pathology 19: 257-262.
• [27] Dwyer J.M., Johnson C. 1981. The use of concanavalin A to study the immunoregulation of human T cells. Clinical and Experimental Immunology 46: 237-249.
• [28] Akira S. 2011. Innate immunity and adjuvants. Philosophical Transactions of the Royal Society B 366: 2748-2755.
• [29] Baskova I.P., Kostrjukova E.S., Vlasova M.A., Kharitonova O.V., Levitskiy S.A., Zavalova L.L., Moshkovskii S.A., Lazarev V.N. 2008. Proteins and peptides of the salivary gland secretion of medicinal leeches Hirudo verbana, H. medicinalis, and H. orientalis. Biochemistry (Moscow) 73: 315-320.
• [30] Antonjuk V.O. 2005. Lektyny ta i’h syrovynni dzherela [Lectins and their sources of raw materials]. Danylo Halytsky Lviv. Nat. Med. Univ., Lviv (In Ukrainian).