Delayed activation of metalloproteinase 9 after photothrombotic stroke in aged mice

• Autorzy: Aleksy M. , Kossut M. , Skangiel-Kramska J.
• Języki publikacji: EN

Abstrakty

EN

Age-related brain injuries, including ischemia, can lead to neurological disabilities, functional decline of the brain and poor recovery. Metalloproteinase-2 (MMP-2) and metalloproteinase-9 (MMP-9) belonging to a large family of zinc-dependent proteinases, play an important role in development and neuronal plasticity, cell migration, angiogenesis and wound healing. After stroke, activation of MMPs is strongly up-regulated. The aim of this study was to compare the time course of activation of MMP- 9 and MMP-2 in the cerebral cortex of young (3ñ4 months old) and aged (12ñ13 months old) mice following focal photothrombotic stroke. To reveal metalloproteinases MMP-9 and MMP-2 activity in situ zymography and SDS-PAGE zymography were used. In young mice, pronounced induction of MMP-9 activity was found one day after stroke and remained at high level 7 and 14 days after infarct. In aged mice, however, MMP-9 activity could be seen not earlier than 14 days post-stroke. Photothrombotic stroke did not affect MMP-2 activity, which remained at stable, constitutive level during whole examined post stroke time period. In both young and aged mice, MMP-2 activity could be detected in sham operated animals, while constitutive MMP-9 activity was not observed. We speculate that the delayed activation of MMP-9 in aged mice may be one of the reasons of lesser ability to recover by old patients. Supported by Polish-German grant nr P-N/026/2006

• Wydawca: -
• Czasopismo: Acta Neurobiologiae Experimentalis
• Rocznik: 2009
• Tom: 69
• Numer: 1
• Opis fizyczny: p.95

Twórcy

autor

Aleksy M.

• Nencki Institute of Experimental Biology, Polish Academy of Sciences, Warsaw, Poland

autor

Kossut M.

• Nencki Institute of Experimental Biology, Polish Academy of Sciences, Warsaw, Poland

autor

Skangiel-Kramska J.

• Nencki Institute of Experimental Biology, Polish Academy of Sciences, Warsaw, Poland