Cadium and zinc-mediated oxidative brust in tobacco BY-2 cell suspension cultures

• Autorzy: Zrobek-Sokolnik A. , Asard H. , Gorska-Koplinska K. , Gorecki R. J.
• Języki publikacji: EN

Abstrakty

EN

Generation of reactive oxygen species (ROS) constitutes an important first reaction under many stress conditions in plants. We demonstrate that Nicotiana tabacum L. cv. Bright Yellow 2 (TBY-2) cells in suspension cultures, generate superoxide radical and hydrogen peroxide upon treatment with cadmium and zinc. Addition of catalase and N,N-diethyldithiocarbamate (DDC) decreased the level of H₂O₂, whereas superoxide dismutase (SOD) induced a slight increase of the H₂O₂ production. The effects of catalase, DDC and SOD on the heavy metal-induced ROS production indicate that it occurs outside of the cells, and that at least part of the hydrogen peroxide is produced by dismutation of the superoxide radical (O₂⁻). The effect of pretreatment of the cell cultures with commonly used mammalian NADPH oxidase inhibitors was also tested. Strong inhibitions of cadmium and zinc-mediated ROS production were obtained with the flavoprotein inhibitors—diphenylene iodonium (DPI) and quinacrine and with an inhibitor of b-type cytochromes—imidazol. Membrane permeable-N-ethyl maleimide (NEM) and iodoacetate, and membrane non-permeable thiol reagents—para-chloromercuribenzoic acid (pCMBS) also inhibited the ROS production. These results suggested that the enzyme responsible for cadmium and zinc-induced ROS production in tobacco cells contains a flavocytochrome. They also show the importance of intra- and extracellular thiol groups in the observed stress reaction. The induction of ROS production with heavy metals showed properties comparable to the elicitor-induced oxidative burst in other plant cells.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2009
• Tom: 31
• Numer: 1
• Opis fizyczny: p.43-49,fig.,ref.

Twórcy

autor

Zrobek-Sokolnik A.

• Chair of Botany and Nature Protection, University of Warmia and Mazury in Olsztyn, Plac Lodzki 1, Olsztyn, Poland

autor

Asard H.

• Department of Biology, Laboratory for Plant Physiology, University of Antwerp, Groenenborgerlaan 171, 2020 Antwerp, Belgium

autor

Gorska-Koplinska K.

• Chair of Plant Physiology and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego 1A, Olsztyn, Poland

autor

Gorecki R. J.

• Chair of Plant Physiology and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego 1A, Olsztyn, Poland

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