Analysis of salinity effects on basil leaf surface area, photosynthetic activity, and growth

• Autorzy: Attia H. , Ouhibi C. , Ellili A. , Msilini N. , Bouzaien G. , Karray N. , Lachaal M.
• Języki publikacji: EN

Abstrakty

EN

Basil (Ocimum basilicum L.) seedlings were cultured on liquid medium in controlled conditions. Two varieties differing in leaf size were compared. When plants were 30 days old, the medium was supplemented with 50 mM NaCl. After 15 days of treatment, root, stemand leaf biomass, leaf number, and leaf surface area were measured. Ion accumulation was determined in roots, stems, and leaves. Photosynthetic parameters (CO₂ fixation rate, internal CO₂ concentration, stomatal conductance) as well as transpiration rate were determined on separate leaves. Electrolyte leakage and malondialdehyde content were used to estimate damage to membranes and lipid peroxidation, respectively. Several antioxidant enzymatic activities were used as proxies of oxidative stress. High Na⁺ concentration was reached in leaf tissues. Salt restricted whole plant biomass deposition rate by diminishing leaf number and leaf expansion, as well as photosynthetic activity were estimated from whole plant biomass production per unit leaf surface area. Diminished stomatal conductance restricted CO₂ fixation rate, and decrease in chlorophyll content presumably limited photosynthetic activity. Lipid peroxidation revealed damages to membranes. The magnitude of these responses differed between the two varieties, indicating that an intraspecific variability in salt response exists in basil.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2011
• Tom: 33
• Numer: 3
• Opis fizyczny: p.823-833,fig.,ref.

Twórcy

autor

Attia H.

• Physiologie et Biochimie de la Tolerance au des Plantes, Faculte des Sciences de Tunis, Campus Universitaire, 2092 Tunis El Manar, Tunisia

autor

Ouhibi C.

• Physiologie et Biochimie de la Tolerance au des Plantes, Faculte des Sciences de Tunis, Campus Universitaire, 2092 Tunis El Manar, Tunisia

autor

Ellili A.

• Physiologie et Biochimie de la Tolerance au des Plantes, Faculte des Sciences de Tunis, Campus Universitaire, 2092 Tunis El Manar, Tunisia

autor

Msilini N.

• Physiologie et Biochimie de la Tolerance au des Plantes, Faculte des Sciences de Tunis, Campus Universitaire, 2092 Tunis El Manar, Tunisia

autor

Bouzaien G.

• Unite d'Agrosylvopastoralisme, Institut National de Recherche en Genie Rural, Eaux et Forets, 2080 Ariana, Tunisia

autor

Karray N.

• Physiologie et Biochimie de la Tolerance au des Plantes, Faculte des Sciences de Tunis, Campus Universitaire, 2092 Tunis El Manar, Tunisia

autor

Lachaal M.

• Physiologie et Biochimie de la Tolerance au des Plantes, Faculte des Sciences de Tunis, Campus Universitaire, 2092 Tunis El Manar, Tunisia

Bibliografia

• Able AJ, Sutherland MW, Guest DI (2003) Production of reactive oxygen species during non-specific elicitation non-host resistance and field resistance expression in cultures of tobacco cells. Funct Plant Biol 30:91–99
• Apel K, Hirt H (2004) Reactive oxygen species: metabolism, oxidative stress, and signal transduction. Annu Rev Plant Biol 55:373–399
• Aphalo PJ (2010) On how to disentangle the contribution of different organs and processes to the growth of whole plants. J Exp Bot 61:626–628
• Ashraf M (2009) Biotechnological approach of improving plant salt tolerance using antioxidants as markers. Biotech Adv 27:84–93
• Attia H, Arnaud N, Karray N, Lachaâl M (2008a) Long-term effects of mild salt stress on growth, ion accumulation and superoxide dismutase expression of Arabidopsis rosette leaves. Physiol Plant 132:293–305
• Attia H, Karray N, Rabhi M, Lachaâl M (2008b) Salt-imposed restrictions on the uptake of macroelements by roots of Arabidopsis thaliana. Acta Physiol Plant 30:23–727
• Attia H, Karray N, Ellili A, Msilini N, Lachaâl M (2009a) Sodium transport in basil. Acta Physiol Plant 31:1045–1051
• Attia H, Karray N, Lachaâl M (2009b) Light interacts with salt stress in regulating superoxide dismutase gene expression in Arabidopsis. Plant Sci 177:161–167
• Bañuls J, Primo-Millo E (1995) Effects of salinity on some Citrus scion-root stock combinations. Ann Bot 76:97–102
• Beauchamp C, Fridovich I (1971) Superoxide dismutase improved assays and applicable to acrylamide gels. Anal Biochem 44:276–287
• Chaparzadeh N, Amico ML, Nejad RK, Izzo R, Izzo FN (2004) Antioxidative responses of Calendula officinalis under salinity conditions. Plant Physiol Biochem 42:695–701
• Chaves MM, Flexas J, Pinheiro C (2009) Photosynthesis under drought and salt stress: regulation mechanisms from whole plant to cell. Ann Bot 103:551–560
• De Pascale S, Maggio A, Fogliano V, Ambrosino P, Ritieni A (2001) Irrigation with saline water improves carotenoids content and antioxidant activity of tomato. J Hort Sci Biotechnol 76:447–453
• Deshpande RS, Tipnis HP (1997) Insecticidal activity of Ocimum basilicum L. Pesticides 11:1–12
• Dionisio-Sese ML, Tobita S (1998) Antioxidant responses of rice seedlings to salinity stress. Plant Sci 135:1–9
• Feierabend J, Dehne S (1996) Fate of the porphyrin cofactors during the light-dependent turnover of catalase and of the photosystem II reaction-center protein D1 in mature rye leaves. Planta 198:413–422
• Flowers TJ, Troke PF, Yeo AR (1977) The mechanism of salt tolerance in halophytes. Annu Rev Plant Physiol 13:75–91
• Flowers TJ, Hajibagheri MA, Yea AR (1991) Ion accumulation in the cell walls of rice plants growing under saline conditions: evidence for the Oertli hypothesis. Pant Cell Environ 14:319–325
• Franck T, Kevers C, Gaspar T (1995) Protective enzymatic systems against activated oxygen species compared in normal and hyperhydric shoots of Prunus avium L. raised in vitro. Plant Growth Regul 16:253–256
• Gossett DR, Millhollon EP, Cran Lucas M (1994) Antioxidant response to NaCl stress in salt-tolerant and salt-sensitive cultivars of cotton. Crop Sci 34:706–714
• Gupta SD, Datta S (2003) Antioxidant enzyme activities during in vitro morphogenesis of gladiolus and the effect of application of antioxidants on plant regeneration. Biol Plant 47:179–183
• Hajibagheri MA, Harvey DMR, Flowers TJ (1987) Quantitative ion distribution within root cells of salt-sensitive and salt-tolerance maize varieties. New Phytol 105:367–379
• Hasegawa PM, Bressan RA, Zhu JK, Bohnert HJ (2000) Plant cellular and molecular responses to high salinity. Annu Rev Plant Physiol Plant Mol Biol 51:463–499
• Heath RL, Packer L (1968) Photoperoxidation in isolated chloroplast. I. Kinetics and stoichiometry of fatty acid peroxidation. Arch Biochem Biophys 125:189–198
• Hernandez JA, Del Rio LA, Sevilla F (1994) Salt stress-induced changes in superoxide dismutase isozymes in leaves and mesophyll protoplasts from Vigna unguiculata (L.) Walp. New Phytol 126:37–44
• Hoagland DR, Arnon DI (1950) The water culture method for growing plants without soil (Circ 347). California Agricultural Experiment Station, Berkley, p 32
• Hunt R (1990) Basic growth analysis. Unwin Hyman, London, p 112
• Javanmardi J, Khalighi A, Kashi A, Bais HP, Vivanco JM (2002) Chemical characterization of basil (Ocimum basilicum L.) found in local accessions and used in traditional medicines in Iran. J Agric Food Chem 50:5878–5883
• Jones HG (1998) Stomatal control of photosynthesis and transpiration. J Exp Bot 49:387–398
• Kaya C, Ak BE, Higgs D, Murillo-Amador B (2002) Influence of foliar applied calcium nitrate on strawberry plants grown under salt stress conditions. Aust J Exp Agric 42:631–636
• Läuchli A, Epstein E (1990) Plant responses to saline and sodic conditions. In: Tanji KK (ed) Agricultural salinity assessment and management. American Society of Civil Engineering, New York, pp 113–137
• Lee DH, Kim YS, Lee CB (2001) The inductive responses of the antioxydant enzymes by salt stress in the rice (Orysa sativa L.). J Plant Physiol 158:737–745
• Leopold AC, Willing RP (1984) Evidence for toxicity effects of salt on membranes. In: Staples RC (ed) Salinity tolerance in plants: strategies for crop improvement. Wiley, New York, pp 67–76
• Levitt J (1972) Salt and ion stresses. In: Physiological ecology: a series of monographs texts and treatises. Academic press, London, pp 489–530
• Lichtenthaler HK (1988) Chlorophylls and carotenoids: pigments of photosynthetic biomembranes. Methods Enzymol 148:350–383
• Lutts S, Kinet JM, Bouharmont J (1996) Effects of salt stress on growth, mineral nutrition and proline accumulation in relation to osmotic adjustment in rice (Oryza sativa L.) cultivars differing in salinity tolerance. Plant Growth Regul 19:207–218
• Majumdar S, Ghosh S, Glick BR, Dumbroff EB (1991) Activities of chlorophyllase, phosphoenolpyruvate carboxylase and ribulose 1, 5 bisphosphate carboxylase in the primary leaves of soybean during senescence and drought. Physiol Plant 81:473–480
• Marschner H (1995) Mineral nutrition of higher plants, 2nd edn. Academic Press, London, p 889
• Matsumoto K, Ohta T, Tanaka T (2005) Dependence of stomatal conductance on leaf chlorophyll concentration and meteorological variables. Agric For Meteorol 132:44–57
• Mittler R (2002) Oxidative stress, antioxidants and stress tolerance. Trends Plant Sci 7:405–410
• Montero E, Cabot C, Barcelo J, Poschenrieder C (1997) Endogenous abscissic acid levels are linked to decrease growth of bush bean plants treated with NaCl. Physiol Plant 101:17–22
• Munns R (2002) Comparative physiology of salt and water stress. Plant Cell Environ 25:239–250
• Munns R, Tester M (2008) Mechanisms of salinity tolerance. Annu Rev Plant Biol 59:651–681
• Nakano Y, Asada K (1981) Hydrogen peroxide in scavenged by ascorbate-specific peroxidise in spinach chloroplast. Plant Cell Physiol 22:867–880
• Nishiyama Y, Yamamoto H, Allakhverdiev SI, Inaba M, Yokota A, Murata N (2001) Oxidative stress inhibits the repair of photodamage to the photosynthetic machinery. EMBO J 20: 5587–5594
• Niyogi KK (1999) Photoprtection revisited. Annu Rev Plant Physiol Plant Mol Biol 50:333–359
• Oertli JJ (1968) Extracellular salt accumulation, a possible mechanism of salt injury in plants. Agrochimica 12:461–469
• Paranychianakis NV, Chartzoulakis KS (2005) Irrigation of Mediterranean crops with saline water: from physiology to management practices. Agric Eco Environ 106:171–187
• Paranychianakis NV, Chartzoulakis KS, Angelakis AN (2004) Influence of rootstock, irrigation level and recycled water on water relations and gas exchange of Soultanina grapevines. Environ Exp Bot 52:185–198
• Polle A (1997) Defense against photooxidative damage in plants. In: Scandalios JG (ed) Oxidative stress and molecular biology of antioxidants defences. Cold Spring Harbor Laboratories, New York, pp 623–666
• Prior LD, Grieve AM, Slavish PG, Gullis PR (1992) Sodium chloride and soil texture interactions in irrigated field grown Sultana grapevines. II. Plant mineral content, growth and physiology. Aust J Agric Res 43:1067–1084
• Putievsky E, Galambosi B (1999) Production systems of sweet basil. In: Hiltunen R, Holm Y (eds) Basil. The genus Ocimum. Harwood Academic Publishers, Amsterdam, pp 39–65
• Reuveni R, Fleischer A, Putievsky E (1984) Fungistatic activity of essential oils from Ocimum basilicum chemotypes. Phytopath Z 110:20–22
• Sen Gupta A, Webb RP, Holladay AS, Allen RD (1993) Overexpression of superoxide dismutase protects plants from oxidative stress. Induction of ascorbate peroxidase in superoxide dismutase overexpressing plants. Plant Physiol 103:1067–1073
• Senatore F (1996) Influence of harvesting time on yield and composition of the essential oil of a thyme (Thymus pulegioides L.) growing wild in Campania (southern Italy). J Agric Food Chem 44:1327–1332
• Shalata A, Neumann PM (2001) Exogenous ascorbic acid (vitamin C) increases resistance to salt stress and reduces lipid peroxidation. J Exp Bot 52:2207–2211
• Shigeoka S, Ishikawa T, Tamoi M, Miyagawa Y, Takeda T, Yabuta Y, Yoshimura K (2002) Regulation and function of ascorbate peroxidase isoenzymes. J Exp Bot 53:1305–1319
• Sibole V, Montero E, Cabot C, Poschenrieder C, Barcelo J (1998) Role of sodium in ABA-mediated long-term growth response of nean to salt stress. Physiol Plant 104:299–305
• Simon JE, Morales MR, Phippen WB, Vieira RF, Hao Z (1999) Basil: a source of aroma compounds and a popular culinary and ornamental herb. In: Janick J (ed) In Perspectives on new crops and new uses. ASHS Press, Alexandria, pp 499–505
• Singh AK, Dubey RS (1995) Changes in chlorophyll a and b contents and activities of photosystems I and II in rice seedlings induced by NaCl. Photosynthetica 31:489–499
• Streb P, Michael-Knauf A, Feierabend J (1993) Preferential photoinactivation of catalase and photoinhibition of photosystem II are common early symptoms under various osmotic and chemical stress conditions. Physiol Plant 88:590–598
• Suarez N (2010) Leaf lifetime photosynthetic rat e and leaf demography in whole plants of Ipomoea pes-caprae growing with a low supply of calcium, a ‘non-mobile’ nutrient. J Exp Bot 61:843–855
• Wahid A (2004) Analysis of toxic and osmotic effects of sodium chloride on leaf growth and economic yield of sugarcane. Bot Bull Acad Sin 45:133–141
• Zhu J (2001) Plant salt tolerance. Plant Sci 6:66–71

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