The utility of Caco-2 cells in isolation of enteroviruses from environmental and clinical material

• Autorzy: Wieczorek M. , Ciacka A. , Witek A. , Litwinska B.
• Języki publikacji: EN

Abstrakty

EN

The work presented here demonstrates the utility of Caco-2 cells in the isolation of enteroviruses (EVs) from environmental and clinical materials. Thirty-two samples of cerebrospinal fluid positive in Pan-entero RT-PCR were taken for EV strain isolation in cell culture. Out of the 32 samples analysed, 22 (68.75%) were positive for enteroviruses by isolation in Caco-2 cells, and 10 (31.25%) were positive by isolation in RD cells. High viral titre in clinical specimens resulted in rate increase for isolation in Caco-2 cells and RD cells (87.5% and 50%, respectively). Also, the probability of isolation of enteroviruses from sewage in Caco-2 cells was 20 times higher that in RD cells. We proved that Caco-2 cells were more effective than RD cells in enterovirus isolation, irrespective of the material used in the inoculation process.

Słowa kluczowe

EN

utility; Caco-2 cell; isolation; enterovirus; environmental material; clinical material; sewage; cerebrospinal fluid

• Wydawca: -
• Czasopismo: Polish Journal of Microbiology
• Rocznik: 2014
• Tom: 63
• Numer: 1
• Opis fizyczny: p.69-73,ref.

Twórcy

autor

Wieczorek M.

• Department of Virology, National Institute of Public Health - National Institute of Hygiene, Chocimska 24 Str., 00-791 Warsaw, Poland

autor

Ciacka A.

• Department of Virology, National Institute of Public Health - National Institute of Hygiene, Chocimska 24 Str., 00-791 Warsaw, Poland

autor

Witek A.

• Department of Virology, National Institute of Public Health - National Institute of Hygiene, Chocimska 24 Str., 00-791 Warsaw, Poland

autor

Litwinska B.

• Department of Virology, National Institute of Public Health - National Institute of Hygiene, Chocimska 24 Str., 00-791 Warsaw, Poland

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