Isolation of Yersinia enterocolitica O:9 from cows positive in serological examination of bovine brucellosis

• Autorzy: Weiner M. , Szulowski K. , Iwaniak W.
• Języki publikacji: EN

Abstrakty

EN

The paper presents the results of bacteriological and molecular investigations on the presence of Y. enterocolitica O:9 in the head, mammary and genital lymph nodes, spleen, liver, and uterus samples originating from 58 cows slaughtered due to the positive results of serological examinations for brucellosis. All samples were cultured for Brucella and Y. enterocolitica and examined in multiplex PCR assay (mPCR), in order to identify the universal 16S rRNA Brucella sp. marker and amplify the perosamine synthetase (per) gene, specific for Y. enterocolitica O:9 only. Out of 58 examined animals, in 23 cases the presence of Y. enterocolitica was demonstrated. Typical Yersinia-suspected colonies were seen after 24-48 h incubation on Cefsulodin-Irgasan- Novobiocin (CIN) Yersinia selective solid medium. The mPCR analysis confirmed the presence of predicted amplicon of 312 bp, typical for Y. enterocolitica O:9 in 20 out of 58 lymph node samples tested and similarly, as in bacteriological examination, other samples were negative. The presence of the 16S rRNA gene of Brucella, generating the amplicon of 905 bp, was not observed in any of the samples tested.

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2012
• Tom: 56
• Numer: 4
• Opis fizyczny: p.473-477,ref.

Twórcy

autor

Weiner M.

• Department of Microbiology, National Veterinary Research Institute, 24-100 Pulawy, Poland

autor

Szulowski K.

• Department of Microbiology, National Veterinary Research Institute, 24-100 Pulawy, Poland

autor

Iwaniak W.

• Department of Microbiology, National Veterinary Research Institute, 24-100 Pulawy, Poland

Bibliografia

• 1. Commission Regulation (EC) No 535/2002 of 21 March 2002 amending Annex C to Council Directive 64/432/EEC and amending Decision 2000/330/EC.
• 2. Corbel M.J., Gill K.P.W., Thomas E.L.: Methods for the identification of Brucella. Central Veterinary Laboratory, New Haw, Weybridge, UK, 1983, pp. 1-63.
• 3. Council Directive 64/432/EEC of 26 June 1964 on animal health problems affecting intra-Community trade in bovine animals and swine. OJ L 121, 29.7.1964, p. 1977.
• 4. Foster G., MacMillan A.P., Godfroid J., Howie F., Ross H.M., Cloeckaert A., Reid R.J., Brew S., Patterson I.A.P.: A review of Brucella sp. infection of sea mammals with particular emphasis on isolates from Scotland, Vet Microbiol 2002, 36, 563-580.
• 5. Gall D., Nielsen K., Forbes L., Davis D., Elzer P., Olsen S., Balsevicius S., Kelly L., Smith P., Tan S., Joly D.: Validation of the fluorescence polarization assay and comparison to other serological assays for the detection of serum antibodies to Brucella abortus in bison. J Wildl Dis 2000, 36, 469-476.
• 6. Garin-Bastuji B., Hummel N., Gerbier G., Cau C., Pouillot R., Da Costa M., Fontaine J.J.: Non specific serological reactions in the diagnosis of bovine brucellosis: experimental oral infection of cattle with repeated doses of Yersinia enterocolilica O:9. Vet Microbiol 1999, 66, 223-233.
• 7. Gerbier G., Garin-Bastuji B., Pouillot R., Véry P., Cau C., Berr V., Dufour B., Moutou F.: False positive serological reactions in bovine brucellosis: evidence of the role of Yersinia enterocolilica serotype O:9 in a field trial. Vet Res 1997, 28, 375-383.
• 8. Grattarola C., Gennero M.S., Zoppi S., Bergagna S., Chiavacci L., Dondo A.: Yersinia enterocolilica and its role in bovine brucellosis monitoring: a statistical analysis. Epidémiol Santé Anim 2006, 50, 153-155.
• 9. Instruction No 26/2003 of the Chief Veterinary Officer, Warsaw, 2003.
• 10. Instruction No 27/2003 of the Chief Veterinary Officer, Warsaw, 2003.
• 11. Instruction No 28/2003 of the Chief Veterinary Officer, Warsaw, 2003.
• 12. Jungersen G., Sørensen V., Giese S.B., Stack J.A., Riber U.: Differentiation between serological responses to Brucella suis and Yersinia enterocolitica serotype O:9 after natural or experimental infection in pigs. Epidemiol Infect 2006, 134, 347-357.
• 13. Konstantinidis A., Minas A., Pournaras S., Kansouzidou A., Papastergiou P., Maniatis A., Stathakis N., Hadjichristodoulou C.: Evaluation and comparison of fluorescence polarization assay with three of the currently used serological tests in diagnosis of human brucellosis. Europ J Clin Microbiol Infect Dis 2007, 26, 715-721.
• 14. Królak M., Stryszak M.: Standard technique of 2- mercaptoethanol test in diagnosis of animal brucellosis. NVRI Pulawy, Poland, 1979.
• 15. Lubeck P.S., Skurnik M., Akrens., Hoorfar J.: A Multiplex PCR-detection assay for Yersinia enterocolilica serotype O:9 and Brucella spp. based on perosamine synthetase gene. Adv Exp Med Biol 2003, 529, 451-455.
• 16. Manual of Diagnostic Tests and Vaccines for Terrestrial Animals. OIE, Paris, 2012. http://www.oie.int/intemational-standard- setting/terrestrial-manual/access-online/
• 17. Nielsen K., Gall D., Bermudez R., Renteria T., Moreno F., Corral A., Monroy O., Monqe F., Smith P., Widdison J., Mardrueno M., Calderon N., Guerrero N., Tinoco R., Osuna J., Kelly W.: Field trial of the brucellosis fluorescence polarization assay. J Immunoassay Immunochem 2002, 23, 307-316.
• 18. Nielsen K., Smith P., Widdison J.. Gall D., Kelly L., Kelly W., Nicoletti P.: Serological relationship between cattle exposed to Brucella abortus, Yersinia enterocolitica O:9 and Escherichia coli O:157:H7. Vet Microbiol 2004, 100, 25-30.
• 19. Pilaszek J., Szulowski K., Iwaniak W.: Epidemiological condition of animal brucellosis in Poland. Medycyna Wet 2000, 56, 363-366.
• 20. Riber U., Jungersen G.: Cell-mediated immune responses differentiate infections with Brucella suis from Yersinia enterocolitica serotype O:9 in pigs. Vet Immunol Immunopathol 2007, 116, 13-25.
• 21. Romero C., Gamazo C., Pardo M., Lopez-Goni I.: Specific detection of Brucella DNA by PCR. J Clin Microbiol 1995, 33, 615-617.
• 22. Scholz H.C., Nöckler K., Göllner C., Bahn P., Vergnaud G., Tomaso H., Al Dahouk S., Kämpfer P., Cloeckaert A., Maquart M., Zygmunt M.S., Whatmore A.M., Pfeffer M., Huber B., Busse H.J., De B.K.: Brucella inopinata sp. nov., isolated from a breast implant infection. Int J Syst Evol Microbiol 2010, 60, 801-808.
• 23. Szulowski K., Iwaniak W., Pilaszek J.: Porcine brucellosis in Poland: problems accompanying serological surveys conducted in 1995-2000. Bull Vet Inst Pulawy 2001, 45, 153-161.
• 24. Szulowski K., Iwaniak W., Weiner M., Złotnicka J., Szymajda M., Zaręba Z., Czępińska H.: Diagnosis and epidemiological condition of bovine brucellosis in Poland. Medycyna Wet 2012, 68, 110-113.
• 25. Szulowski K., Iwaniak W., Złotnicka J., Weiner M., Zaręba Z., Czępińska H.: International trade - a potential source of brucellosis in pigs. Medycyna Wet 2011, 67, 64-66.
• 26. Weiner M., Złotnicka J., Iwaniak W., Szulowski K.: Development of a multiplex PCR for identification of Brucella sp. and cross-reacting Yersinia enterocolitis O:9. Bull Vet Inst Pulawy 2011, 55, 603-607.
• 27. Weynants V., Tibor A., Denoel P.A., Saegerman C., Godfroid J., Thiange P., Letesson J.J.: Infection of cattle with Yersinia enterocolitica O:9 a cause of the false positive serological reactions in bovine brucellosis diagnostic tests. Vet Microbiol 1996, 48, 101-112.
• 28. Wiśniowski J., Królak M., Drożdżyńska M.: Standard technique of anti-globulin test in diagnosis of bovine brucellosis. NVRI Pulawy, Poland. 1978.