Patterned growth and differentiation of human cord blood-derived neural stem cells on bio-functionalized surfaces

• Autorzy: Buzanska L. , Ruiz A. , Zychowicz M. , Rauscher H. , Ceriotti L. , Rossi F. , Colpo P. , Domanska-Janik K. , Coecke S.
• Języki publikacji: EN

Abstrakty

EN

Bio-functionalized surfaces were prepared to study the adherence and differentiation capacity of neural stem cells derived from human umbilical cord blood (HUCB-NSC). Cell growth platforms containing arranged arrays of adhesive molecules were created by microcontact printing on a biologically inert surface. Biomolecules used to prepare microarray platforms included the extracellular matrix protein fibronectin and the polyaminoacid poly-L-lysine. HUCB-NSC plated on microplatforms at various serum conditions showed serum and molecule type dependent capacity for adhesion and differentiation. Poly-L-lysine allowed the maintenance of stem-like non differentiated cells attached to the surface, whereas fibronectin promoted spreading and neural commitment. Serum deprivation did not influence the attachment of HUCB-NSC to fibronectin, but significantly enhanced the attachment to poly-L-lysine and promoted dBcAMP induced neuronal differentiation. A bio-pattern of squares with interconnecting lines was used to guide neuronal differentiation by directing cell protrusion outgrowth. Tailoring the geometry of the bio-pattern enabled directing and monitoring of the neural stem cells development in the large scale multiparameter biotests.

• Wydawca: -
• Czasopismo: Acta Neurobiologiae Experimentalis
• Rocznik: 2009
• Tom: 69
• Numer: 1
• Opis fizyczny: p.24-36,fig.,ref.

Twórcy

autor

Buzanska L.

• In Vitro Toxicology/ECVAM, Institute for Health and Consumer Protection, European Commission, Joint Research Centre, Ispra, Italy
• NeuroRepair Department, Mossakowski Medical Research Centre, Polish Academy of Sciences, Warsaw, Poland

autor

Ruiz A.

• Nanotechnology and Molecular Imaging Unit, Institute for Health and Consumer Protection, Joint Research Centre, European Commission, Ispra, Italy

autor

Zychowicz M.

• NeuroRepair Department, Mossakowski Medical Research Centre, Polish Academy of Sciences, Warsaw, Poland
• Nanotechnology and Molecular Imaging Unit, Institute for Health and Consumer Protection, Joint Research Centre, European Commission, Ispra, Italy

autor

Rauscher H.

• Nanotechnology and Molecular Imaging Unit, Institute for Health and Consumer Protection, Joint Research Centre, European Commission, Ispra, Italy

autor

Ceriotti L.

• Nanotechnology and Molecular Imaging Unit, Institute for Health and Consumer Protection, Joint Research Centre, European Commission, Ispra, Italy

autor

Rossi F.

• Nanotechnology and Molecular Imaging Unit, Institute for Health and Consumer Protection, Joint Research Centre, European Commission, Ispra, Italy

autor

Colpo P.

• Nanotechnology and Molecular Imaging Unit, Institute for Health and Consumer Protection, Joint Research Centre, European Commission, Ispra, Italy

autor

Domanska-Janik K.

• NeuroRepair Department, Mossakowski Medical Research Centre, Polish Academy of Sciences, Warsaw, Poland

autor

Coecke S.

• In Vitro Toxicology/ECVAM, Institute for Health and Consumer Protection, European Commission, Joint Research Centre, Ispra, Italy

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