Photothrombotic stroke-induced changes in expression level of genes coding for enzymes associted with hyaluronic acid metabolism in the mouse brain

• Autorzy: Greda A.K. , Nowicka D.
• Języki publikacji: EN

Abstrakty

EN

BACKGROUND AND AIMS: Perineuronal nets (PNNs) are complex structures of the nervous system extracellular matrix, composed of hyaluronic acid and chondroitin sulfate proteoglycans. PNNs surround mostly the subset of parvalbumin-expressing interneurons by enwrapping their cell body and proximal dendrites. Their presence stabilizes existing synapses and limits neuronal plasticity. Mechanisms that regulate PNNs expression remain unclear. Changes in PNNs expression are observed in perilesional area after photothrombosis and can be considered an attempt to create conditions favorable for synaptic remodeling. We hypothesize that they may result from the activity of endogenous glycolytic enzymes. METHODS: In the present study, employing quantitative RT-qPCR, we analyzed the expression of genes coding for hyaluronic acid synthesizing and degrading enzymes in cortical region of the perilesional area at 1 h, 24 h and 7 days after photothrombotic stroke. RESULTS: Analysis revealed substantial increase in hyaluronidase 1 (HYAL1) expression level in comparison to homotopic contralateral cortical region and control animals. The rise was recognized at 24 h after stroke and maintained up to 7 days after stroke. No change in expression level of HYAL2 was detected. While 24 h after photothrombosis the elevation in hyaluronic acid synthase 2 (HAS2) expression level was observed, the decrease in HAS3 expression level occurred. The expression level of HAS1 maintained unchanged after stroke. CONCLUSIONS: The obtained data indicate photothrombotic stroke-evoked modification of hyaluronic acid component of PNNs in perilesional area. The results show parallel processes of degradation and synthesis at 24 h, which may exert different effects on neuronal plasticity.  This work was supported by NCN grant 2012/05/B/NZ3/00851.

• Wydawca: -
• Czasopismo: Acta Neurobiologiae Experimentalis
• Rocznik: 2015
• Tom: 75
• Numer: Supl.
• Opis fizyczny: p.S62-S63

Twórcy

autor

Greda A.K.

• Nencki Institute of Experimental Biology, Polish Academy of Sciences, Warsaw, Poland

autor

Nowicka D.

• Nencki Institute of Experimental Biology, Polish Academy of Sciences, Warsaw, Poland