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2014 | 58 | 4 |

Tytuł artykułu

Genotypic virulence markers of Yersinia enterocolitica O:9 isolated from pigs and wild boars serologically positive and negative for brucellosis

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
The paper concerns molecular study on pathogenicity markers of fourteen Y. enterocolitica 0:9 isolated from pigs in which initially positive serological reactions for brucellosis were observed (n = 41), health) pigs, which were brucellosis-negative (n = 258), and wild boars serologically negative for brucellosis (n = 209). PCR identification proved that all isolates were ail, ystA- and myfA-positive. The plasmid encoding yadA marker was detected in nine isolates that originated from pigs serologically, positive or negative for brucellosis, and from one isolate of wild boar origin. Furthermore, none of the examined isolates was ystB-positive. Results of the investigations indicate that the Y enterocolitica 0:9 isolates from pigs or wild boars, regardless of whether they were serologically positive or negative for brucellosis, may also be potentially pathogenic for humans, due to the presence of chromosomal and plasmid encoded molecular markers.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

58

Numer

4

Opis fizyczny

p.541-545,ref.

Twórcy

autor
  • Department of Microbiology, National Veterinary Research Institute, 24-100 Pulawy, Poland
autor
  • Department of Microbiology, National Veterinary Research Institute, 24-100 Pulawy, Poland
autor
  • Department of Microbiology, National Veterinary Research Institute, 24-100 Pulawy, Poland
autor
  • Department of Microbiology, National Veterinary Research Institute, 24-100 Pulawy, Poland
autor
  • Department of Microbiology, National Veterinary Research Institute, 24-100 Pulawy, Poland
autor
  • Department of Microbiology, National Veterinary Research Institute, 24-100 Pulawy, Poland

Bibliografia

  • 1. Cvetnic Z., Mitak M., Ocepek O., Lojkic M., Terzic S., Jemersic L., Humski A., Habrun B., Sostaric B., Brstilo M., Krt B., Garin-Bastuji B.: Wild boars (Sus scrofa) as reservoirs of Brucella suis biovar 2 in Croatia. Acta Vet Hung 2003, 51, 465-473.
  • 2. European Economic Community. Council Directive 90/429/EEC of 26 June 1990 laving down the animal health requirements applicable to intra- Community trade in and imports of semen of domestic animals of the porcine species.
  • 3. Gierczyński R., Jagielski M., Rastawicki W.: Evaluation of usefulness for selected virulence markers for identifying pathogenic Yersinia enterocolitis strains. IV. Genes myfA and myfC and ureC. Med Dosw Mikrobiol 2002, 54, 347-355.
  • 4. Gyuranecz M., Erdelyi K., Makrai L., Fodor L., Szepe B., Raczne Meszaros A., Dan A., Dencso L., Fassang E., Szeredi L.: Brucellosis of the European brown hare (Lepus europaeus). J Comp Path 2011, 145, 1-5.
  • 5. Harnett N., Lin Y.P., Krishahn C.: Detection of pathogenic Yersinia enterocolitis using the multiplex polymerase chain reaction. Epidemiol Infect 1996, 117, 59-67.
  • 6. Hilbink F., Fenwick S.G., Thompson E.J., Kittelberger R., Penrose M., Ross G.P.: Non-specific seroreactions against Brucella abortus in ruminants in New Zealand and the presence of Yersinia enterocolitica 0:9. N Z Vet J 1995, 43, 175-178.
  • 7. Instruction No. 401/BB-16/2010 of the Chief Veterinary Officer, Warsaw, 2010.
  • 8. Iriarte M., Cornells G.R.: MyfF, an element of the network regulating the synthesis of fibriallae in Yersinia enterocolitica. J Bacteriol 1995, 177, 738-744.
  • 9. Jungersen G., Sorensen V., Glese S.B., Stack A., Riber U.: Differentiation between serological responses to Brucella suis and Yersinia enterocolitica serotype O:9 after natural or experimental infection in pigs. Epidemiol Infect 2006, 134, 347-357.
  • 10. Kot B., Piechota M., Jakubczak A.: Analysis of occurrence of virulence genes among Yersinia enterocolitica isolates belonging to different biotypes and serotypes. Pol J Vet Sci 2010, 13, 13-19.
  • 11. Kot B.: Chromosomally and plasmid-encoded virulence determinants of Y. enterocolitica. Med Weter 2010, 66, 294-298.
  • 12. Lubeck P.S., Skurnik M., Akrens., Hoorfar J.: A multiplex PCR- detection assay for Yersinia enterocolitica serotype 0:9 and Brucella spp. based on perosamine syntethase gene. Adv Exp Med Biol 2003, 529, 451-455.
  • 13. Manual of Diagnostic Tests and Vaccines for Terrestrial Animals, OIE, Paris, 2010 http://www.oie.int/international- standard-setting/terrestrial-manual/access-online/
  • 14. Nielsen K., Smith P., Widdison J., Gall D., Kelly L., Kelly W., Nicoletti P.: Serological relationship between cattle exposed to Brucella abortus, Yersinia enterocolitica O:9 and Escherichia coli O:157:H7. Vet Microbiol 2004, 100, 25-30.
  • 15. Platt-Samoraj A., Ugorski M., Szweda W., Szczerba-Turek A., Wojciech K., Procajło Z.: Analysis of the presence of ail, ystA and ystB genes in Yersinia enterocolitica strains isolated from aborting sows and aborted fetuses. J Vet Med B 2006, 53, 341-346.
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  • 19. Szulowski K., Iwaniak W., Weiner M., Złotnicka J., Szymajda M., Zaręba Z., Czępińska H.: Diagnosis and epidemiological condition of bovine brucellosis in Poland. Med Weter 2012, 68, 110-113.
  • 20. Thoerner P., Bin Kingombe C.I., Bogli-Stuber K., Bissig-Choisant B., Wassenaar T.M., Frey J., Jemmi T.: PCR detection for virulence genes in Yersinia enlerocolitica and Yersinia pseudotuberculosis and investigation of virulence gene distribution. Appl Environ Microbiol 2003, 69, 1810-1816.
  • 21. Wannet W.J., Reessink M., Brunings H.A., Maas H.M.: Detection of pathogenic Yersinia enlerocolitica by a rapid and sensitive duplex PCR. J Clin Microbiol 2001, 39, 4483-4486.
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  • 23. Weiner M., Złotnicka J., Iwaniak W., Szulowski K.: Development of a multiplex PCR for identification of Brucella sp. and cross-reacting Yersinia enlerocolitica O:9. Bull Vet Inst Pulawy 2011, 55, 603-607.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

bwmeta1.element.agro-8b222199-6b2e-42ef-9e01-9ff9b3c01eef
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