Application of a real-time PCR method for Salmonella spp., Escherichia coli, Staphylococcus aureus and Clostridium perfringens detection in water samples

• Autorzy: Rozwadowska B. , Albertynska M. , Hudzik G. , Okla H. , Jasik K. P. , Slodki J.
• Języki publikacji: EN

Abstrakty

EN

The diagnostic assessment of water sanitary state is based mainly on the cultivation of bacteria retained on membrane filters. However classical microbiology methods have a lot of disadvantages. More and more frequently, rapid detection and identification of pathogens present in water is based on molecular biology techniques. The aim of this study was to determine the effectiveness and usefulness of a real-time PCR method, when compared to the recommended bacteria culture method, in diagnostics of pathogens in water samples. The research concerned the detection and identification of main sanitary indicators of water such as: Salmonella spp., Escherichia coli, Staphylococcus aureus and Clostridium perfringens. The analyses were conducted in water samples contaminated with the reference material (the aforementioned bacteria) and real environmental samples, which were examined for the presence of nucleic acid of: Salmonella spp., E. coli, S. aureus and C. perfringens using a real-time PCR method.

Słowa kluczowe

EN

application; real-time polymerase chain reaction; Salmonella; Escherichia coli; Staphylococcus aureus; Clostridium perfringens; detection; water sample

• Wydawca: -
• Czasopismo: Polish Journal of Microbiology
• Rocznik: 2013
• Tom: 62
• Numer: 4
• Opis fizyczny: p.439-443,ref.

Twórcy

autor

Rozwadowska B.

• Provincial Sanitary and Epidemiological Station in Katowice, Raciborska 39, 40-957 Katowice, Poland

autor

Albertynska M.

• Provincial Sanitary and Epidemiological Station in Katowice, Raciborska 39, 40-957 Katowice, Poland

autor

Hudzik G.

• Provincial Sanitary and Epidemiological Station in Katowice, Raciborska 39, 40-957 Katowice, Poland

autor

Okla H.

• Medical University of Silesia in Katowice, Division of Laboratory Medicine in Sosnowiec, Sosnowiec, Poland

autor

Jasik K. P.

• Medical University of Silesia in Katowice, Division of Laboratory Medicine in Sosnowiec, Sosnowiec, Poland

autor

Slodki J.

• Medical University of Silesia in Katowice, Division of Laboratory Medicine in Sosnowiec, Sosnowiec, Poland

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