Gene expression and morphological characterization of cell suspensions of Coffea arabica L. cv. Catiguá MG2 in different cultivation stages

• Autorzy: Torres L.F. , Diniz L.E.C. , Do Livramento K.G. , Freire L.L. , Paiva L.V.
• Języki publikacji: EN

Abstrakty

EN

The present study aimed to evaluate the quality of cell suspension of Coffea arabica cv. Catiguá at four different ages by morphological and gene expression analysis. For this purpose, cell suspension samples were collected at 30, 45, 60 and 75 days. Morphological analysis revealed the presence of embryogenic regions in all samples, and after 60 days, non-embryogenic regions were also identified. The genic analyses revealed that as the cells were transferred to a new medium a change in both their physical and chemical conditions was noticed that caused stress. The decrease in SERK and BBM genes expression after 75th day may also be due to the non-embryogenic regions, characterized by large, elongated and vacuolated cells that were observed in the periphery of embryogenic regions, starting from 60 days of culture.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2015
• Tom: 37
• Numer: 09
• Opis fizyczny: Article: 175 [7 p.], fig.,ref.

Twórcy

autor

Torres L.F.

• Departamento de Quimica/Laboratorio Central de Biologia Molecular, Universidade Federal de Lavras, Campus Universitario, Lavras, MG, CEP 37200-000, Brazil

autor

Diniz L.E.C.

• Embrapa Tabuleiros Costeiros/Universidade Tiradentes, Avenida Beira Mar 3250, Bairro Jardins, Aracaju, SE, CEP 49025-040, Brazil

autor

Do Livramento K.G.

• Departamento de Quimica/Laboratorio Central de Biologia Molecular, Universidade Federal de Lavras, Campus Universitario, Lavras, MG, CEP 37200-000, Brazil

autor

Freire L.L.

• Departamento de Quimica/Laboratorio Central de Biologia Molecular, Universidade Federal de Lavras, Campus Universitario, Lavras, MG, CEP 37200-000, Brazil

autor

Paiva L.V.

• Departamento de Quimica/Laboratorio Central de Biologia Molecular, Universidade Federal de Lavras, Campus Universitario, Lavras, MG, CEP 37200-000, Brazil

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Identyfikatory

DOI

10.1007/s11738-015-1924-6