EN
Oxtetracycline (Terramycin) medicated sirup, extender patties or dust have been shown to be effective in controlling of American foulbrood in hee colonies in Finland and in Poland. No residues of oxytetracycline were found in surplus honey from 103 randomely chosen colonies treated with the antibiotic at doses from 150 to 600 mg/colony. The therapy should start at least 30 days before honey, extraction to ovoid contamination of this food product with oxytetracycline. A high performance liquid chromatography (HPLC) was used to detect oxytetracyline in honey samples. The separation was performed on a steel column packed with Li Chromosorb RP-10 (10 µm) with methanol-acetonitrile-0.01 N aqueous hydrochloric acid (1:1:4) as the mobile phase at a flow rate of 0.7 ml/min at room temperature. 0.1 g sample of honey was dissolved in 20 ml of 0.1 M Na₂EDTA- Mc Illvain buffer (pH 4.0) and cleaned to remove many peaks of interferring substances appearing in honey. The detection limits is 0.02 ppm for oxytetracycline in honey.