Biosynthesis of myo-inositol in jycopods: characteristics of the pteridophytic L-Myo-inositol-1-phosphate synthase and myo-inositol-1-phosphate phosphatase from the strobili of Lycopodium clavatum and Selaginella monospora
Two key enzymes of myo-inositol metabolism [L-myo-inositol-1-phosphate synthase (MIPS) and myoinositol-1-phosphate-phosphatase (MIPP)] were identified for the first time in Pteridophyta in Lycopodium clavatum and Selaginella monospora. Both enzymes from both plants were partly purified and the degree of purity obtained from 47 to 75 folds. MIPS preparations specifically utilized D-glucose-6-phosphate and NAD⁺ as its substrate and coenzyme with the Km values for G-6-P and NAD⁺ were 1.74 and 0.34 mM in L. clavatum; 2.32 and 0.37 mM in S. monospora. MIPP preparations used D/Lmyo-inositol-1-phosphate as its principal substrate with the Km values for MIP was 0.068 mM in L. clavatum and 0.076 mM in S. monospora. The pH reliance of all the preparations was around 7.0–7.5 and different cations had variable role.
Biochemistry Laboratory, Department of Botany, Presidency University, 86/1 College Street, 700 073 Kolkata, India
Bibliografia
Adhikari J, Majumder AL (1983) Differences in thermal stability of the fetal and adult brain myo-inositol-1-phosphate synthase. FEBS Lett 163:46–49
Adhikari J, Majumder AL, Bhaduri TJ, Dasgupta S, Majumder AL (1987) Chloroplast as a locale of L–myo-inositol–1-phosphate synthase. Plant Physiol 85:611–614
Attwood PV, Ducep JB, Chanal MC (1988) Purification and properties of myo-inositol-1-phosphatase from bovine brain. Biochem J 253:387–394
Banerjee R, Chhetri DR, Adhikari J (2007) Occurrence of myoinositol-1-phosphate phosphatase in pteridophytes: characteristics of the enzyme from the reproductive pinnules of Dryopteris filix-mas (L.) Schott. Braz. J Plant Physiol 19(2):109–117
Barnett JEG, Brice RE, Corina DL (1970) A colorimetric determination of inositol monophosphates as an assay for D-Glucose–6-phosphate–1L-myo-Inositol-1-phosphate cyclase. Biochem J 119:183–186
Benaroya RO, Zamski E, Tel-Or E (2004) L-myo-inositol-1- phosphate synthase in the aquatic fern Azolla filiculoides. Plant Physiol Biochem 42:97–102
Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of proteins utilizing principle of protein–dye binding. Anal Biochem 72:248–254
Chen H, Xiong L (2010) myo-Inositol-1-phosphate synthase is required for polar auxin transport and organ development. J Biol Chem 285(31):24238–24247
Chen RS Jr, Toribara TY, Warner H (1956) Microdetermination of phosphorous. Anal Chem 28:1756–1758
Chen L, Zhou C, Yang H, Roberts MF (2000) Inositol-1-phosphate synthase from Archaeoglobus fulgidus is a class II aldolase. Biochemistry 39:12415–12423
Chettri DR, Mukherjee AK, Adhikari J (2006) Partial purification and characterization of L-myo-inositol-1-phosphate synthase of pteridophytic origin. Acta Physiol Plant 28:101–107
Dasgupta S, Adhikari J, Majumder AL (1984) Myo-inositol-1-phosphate synthase from lower plant groups: purification and properties of the enzyme from Euglena gracilis. Physiol Plant 61:412–416
Donahue TF, Henry SA (1981) Myo-inositol-1-phosphate synthase: characteristics of the enzyme and identification of its structural gene in yeast. J Biol Chem 256:7077–7085
Eisenberg F Jr (1967) D-myo-inositol-1-phosphate as product of cyclization glucose-6-phosphate and substrate for a specific phosphatase in rat testis. J Biol Chem 242:1375–1382
Escamilla JE, Contreas M, Martinez A, Pina MZ (1982) L-myoinositol-1-phosphate synthase from Neurospora crassa: purification to homogeneity and partial characterization. Arch Biochem Biophys 218:275–285
Fujimoto S, Okano I, Tanaka Y, Sumida Y, Tsuda J, Kawakami N, Shimohama S (1996) Zinc-ion-dependent acid phosphatase exhibits magnesium-ion-dependent myo-inositol-1-phosphatase activity. Biol Pharma Bull 19:882–885
Gee NS, Ragan CI, Watling KJ, Aspley S, Jackson RG, Reid GG, Gani D, Shute JK (1988) The purification and properties of myoinositol monophosphatase from bovine brain. Biochem J 249:883–889
Gumber SC, Loewus MW, Loewus FA (1984) Myo-inositol-1-phosphate synthase from pine pollen: sulfhydryl involvement at the active site. Arch Biochem Biophys 231:372–377
Johnson MD, Sussex IM (1995) 1L–myo-inositol-1-phosphate synthase from Arabidopsis thaliana. Plant Physiol 107:613–619
Johnson MD, Wang X (1996) Differentially expressed forms of 1-Lmyo-Inositol-1-phosphate synthase (EC. 5.5.1.4) in Phaseolus vulgaris. J Biol Chem 271:17215–17218
Kwok F, Lo SC (1994) Development of a continuous coupled enzymatic assay for myo-inositol monophosphatase. J Biochem Biophys Meth 29:173–178
Leech AP, Baker GR, Shute JK, Cohen MA, Gani D (1993) Chemical and kinetic mechanism of the inositol monophosphatase reaction and its inhibition by Li+. Eur J Biochem 212:693–704
Loewus FA, Loewus MW (1980) Myo-inositol: biosynthesis and metabolism. In: Preiss J (ed) The biochemistry of plants: carbohydrate structure and function, vol 3. Academic Press, New York, pp 43–76
Loewus FA, Loewus MW (1983) Myo-inositol: its biosynthesis and metabolism. Ann Rev Plant Physiol 34:137–161
Lohia A, Hait NC, Majumder AL (1999) L-myo-inositol-1-phosphate synthase from Entamoeba histolytica. Mol Biochem Parasitol 98:67–79
Maeda T, Eisenberg F Jr (1980) Purification, structure and catalytic properties of the 1 L-myo-inositol-1-phosphate synthase from rat testis. J Biol Chem 255:8458–8464
Majumder AL, Biswas BB (2006) Biology of inositols and phosphoinositides. (Subcellular biochemistry). Springer, USA
Majumder AL, Chatterjee A, Dastidar KG, Majee M (2003) Diversification and evolution of L–myo-inositol-1-phosphate synthase. FEBS Lett 553:3–10
Ogunyemi EO, Pittner F, Hoffmann-Ostenhof O (1978) Studies on the biosynthesis of cyclitols. XXXVI. Purification of myo-inositol-1-phosphate synthase of the duckweed Lemna gibba to homogeneity by affinity chromatography on NAD sepharose. Molecular and catalytic properties of the enzyme. Hoppe Seyler’s Z Physiol Chem 359:613–616