Influence of salidroside, a neuroactive compound of Rhodiola rosea L., on alcohol tolerance development in rats

• Autorzy: Szulc M. , Mularczyk P. , Kujawski R. , Gryszczynska A. , Kaminska E. , Geppert B. , Baraniak J. , Kania- Dobrowolska M. , Ozarowski M. , Krajewska-Patan A. , Mikolajczak P.L.

Warianty tytułu

PL

Wpływ salidrozydu, neuroaktywnego związku Rhodiola rosea L. na rozwój tolerancji alkoholu u szczurów

• Języki publikacji: EN

Abstrakty

EN

In recent years, the search for potential neuroprotective properties of salidroside and its ability to influence the activity of nervous system become the subject of intense studies of many research groups. None of these studies, however, include an attempt to determine the effect of salidroside on the course of alcohol tolerance in vivo. The aim of this study was to investigate the ability of salidroside to inhibit the development of alcohol tolerance in rats, determining whether the effect of its action may occur in a dose-dependent manner, reducing both metabolic and central tolerance without affecting body temperature in control rats. Male Wistar rats were injected daily with ethanol at a dose of 3 g/kg for 9 consecutive days to produce ethanol tolerance. Salidroside in two doses (4.5 mg/kg and 45 mg/kg b.w.) or vehiculum was administered orally. On the 1st, 3rd, 5th and 8th day a hypothermic effect of ethanol was measured, while the loss of righting reflex procedure was performed on the 2nd, 4th, 6th and 7th day. On the 9th day rats were treated with salidroside, sacrificed 1 h after ethanol injections and blood was collected for blood-ethanol concentration measurement. Salidroside at a dose of 45 mg/kg inhibited the development of tolerance to hypothermic and sedative effects of ethanol, whereas insignificant elevation of blood-ethanol concentration was observed. The dose of 4.5 mg/kg b.w. had minimal effect, only small inhibition of tolerance to hypothermic action was observed. Salidroside affected neither body mass growth nor body temperature in non-alcoholic (control) rats.Results of the study indicate that salidroside at a dose of 45 mg/kg inhibited the development of tolerance to the hypothermic effect of ethanol. Observed inhibition of tolerance to the sedative effect of ethanol seems to be associated with salidroside influence on the central nervous system. A comprehensive explanation of the abovementioned observations requires further pharmacological and pharmacodynamic studies.

PL

W ostatnich latach poszukiwanie potencjalnych właściwości neuroprotekcyjnych salidrozydu i jego zdolności do wpływania na aktywność układu nerwowego stało się przedmiotem intensywnych badań wielu grup naukowców. Żadne z tych badań nie obejmowało jednak próby określenia działania tego związku na przebieg tolerancji alkoholowej in vivo. Celem doświadczenia była ocena zdolności salidrozydu do hamowania rozwoju tolerancji na alkohol u szczurów oraz ustalenie czy efekt jego działania może wystąpić w sposób zależny od dawki, czy może znosić tolerancję metaboliczną i centralną bez wpływu na temperaturę ciała u zwierząt. Samcom szczurów szczepu Wistar codziennie podawano etanol w dawce 3 g/kg m.c. i.p. przez 9 kolejnych dni w celu uzyskania tolerancji jego obecności. Salidrozyd w dwóch dawkach (4,5 mg/kg i 45 mg/ kg m.c.) lub vehiculum podawano i.g. W kolejnych dniach mierzono hipotermiczny efekt działania etanolu oraz utratę odruchu utrzymania postawy ciała. Ostatniego dnia po dekapitacji zwierząt pobrano krew w celu pomiaru stężenia we krwi etanolu. Salidrozyd w dawce 45 mg/kg hamował rozwój tolerancji objawiający się hipotermią i uspokajającym działaniem etanolu, podczas gdy obserwowano nieznaczne zwiększenie stężenia etanolu we krwi. Dawka 4,5 mg/kg m.c. wykazała minimalny efekt, obserwowano jedynie niewielkie zahamowanie tolerancji na działanie hipotermiczne. Salidrozyd nie wpływał ani na przyrost masy ciała ani na temperaturę ciała u szczurów nie otrzymujących alkoholu (kontrolnych). W badanym układzie doświadczalnym z wykorzystaniem eksperymentalnego modelu tolerancji potwierdzono hamujący wpływ salidrozydu w dawce 45 mg/kg m.c. na rozwój tego procesu. Hamowanie tolerancji działania sedacyjnego etanolu wydaje się być związane z wpływem salidrozydu na ośrodkowy układ nerwowy. Kompleksowe wyjaśnienie powyższych obserwacji wymaga dalszych badań farmakologicznych i farmakodynamicznych.

Słowa kluczowe

EN

salidroside; neuroactive substance; Rhodiola rosea; alcohol tolerance; development; rat

• Wydawca: -
• Czasopismo: Herba Polonica
• Rocznik: 2018
• Tom: 64
• Numer: 1
• Opis fizyczny: p.22-35,fig.,ref.

Twórcy

autor

Szulc M.

• Department of Pharmacology, Poznan University of Medical Sciences, Rokietnicka 5a, 60-806 Poznan, Poland

autor

Mularczyk P.

• Department of Pharmacology, Poznań University of Medical Sciences, Rokietnicka 5a, 60-806 Poznan, Poland

autor

Kujawski R.

• Department of Pharmacology, Poznań University of Medical Sciences, Rokietnicka 5a, 60-806 Poznan, Poland

autor

Gryszczynska A.

• Department of Pharmacology and Phytochemistry, Institute of Natural Fibres and Medicinal Plants, Kolejowa 2a 62-064 Plewiska, Poland

autor

Kaminska E.

• Department of Pharmacology, Poznan University of Medical Sciences, Rokietnicka 5a, 60-806 Poznan, Poland

autor

Geppert B.

• Department of Pharmacology and Phytochemistry, Institute of Natural Fibres and Medicinal Plants, Kolejowa 2a, 62-064 Plewiska, Poland

autor

Baraniak J.

• Department of Pharmacology and Phytochemistry, Institute of Natural Fibres and Medicinal Plants, Kolejowa 2a, 62-064 Plewiska, Poland

autor

Kania- Dobrowolska M.

• Department of Pharmacology and Phytochemistry, Institute of Natural Fibres and Medicinal Plants, Kolejowa 2a, 62-064 Plewiska, Poland

autor

Ozarowski M.

• Department of Pharmacology and Phytochemistry, Institute of Natural Fibres and Medicinal Plants, Kolejowa 2a, 62-064 Plewiska, Poland
• Department of Pharmaceutical Botany and Plant Biotechnology, Poznan University of Medical Sciences, Sw. Marii Magdaleny 14, 61-861 Poznań, Poland

autor

Krajewska-Patan A.

• Department of Botany, Breeding and Agricultural Technology of Medicinal Plants, Institute of Natural Fibres and Medicinal Plants, Kolejowa 2a, 62-064 Plewiska, Poland

autor

Mikolajczak P.L.

• Department of Pharmacology, Poznan University of Medical Sciences, Rokietnicka 5a, 60-806 Poznan, Poland
• Department of Pharmacology and Phytochemistry, Institute of Natural Fibres and Medicinal Plants, Kolejowa 2a, 62-064 Plewiska, Poland

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Identyfikatory

DOI

10.2478/hepo-2018-0002