Quantification of bovine mammary stem/progenitor cells by laser scanning and flow cytometry

• Autorzy: Osinska E. , Gajewska M. , Majewska A. , Motyl T.
• Języki publikacji: EN

Abstrakty

EN

Due to the presence and activity of mammary stem cells (MaSC), growth and remodelling of mammary gland during puberty and lactation cycles is possible. In this study the number of putative mammary stem/progenitor cells was examined in 20 months old non-pregnant Holstein-Friesian heifers. Cells were double-stained with fluorescent dye-conjugated antibodies against stem cell antigen-1 (Sca-1) and fibronectin type III domain containing 3B (FNDC3B), and were analysed Rusing scanning cytometry and flow cytometry. Nuclei were counterstained with Hoechst 33342. Scanning and flow cytometry revealed 2.43±0.32% and 1±0.37% of MaSC in total cell number, respectively.Sca-1posFNDC3Bpos cells did not express estrogen receptor (ERα), confirming their undifferentiated phenotype. In conclusion, scanning cytometry is a preferable method for evaluation of the number and localization of MaSC in situ, whereas, flow cytometry with cell sorting enables further genomic and biochemical analyses of isolated cells.

Słowa kluczowe

EN

quantification; mammary gland; progenitor cell; laser scanning; flow cytometry; stem

• Wydawca: -
• Czasopismo: Animal Science Papers and Reports
• Rocznik: 2015
• Tom: 33
• Numer: 1
• Opis fizyczny: p.5-11,fig.,ref.

Twórcy

autor

Osinska E.

• Department of Physiological Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences - SGGW, Nowoursynowska 159, 02-776 Warsaw, Poland

autor

Gajewska M.

• Department of Physiological Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences - SGGW, Nowoursynowska 159, 02-776 Warsaw, Poland

autor

Majewska A.

• Department of Physiological Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences - SGGW, Nowoursynowska 159, 02-776 Warsaw, Poland

autor

Motyl T.

• Department of Physiological Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences - SGGW, Nowoursynowska 159, 02-776 Warsaw, Poland

Bibliografia

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